Isolation and Identification of Arsenic-Resistant Extremophilic Bacteria from the Crater-Lake Volcano “El Chichon”, Mexico

Ovando-Ovando, Cesar Ivan; Feregrino-Mondragón, R. Daniela; Rincón-Rosales, Reiner; Jasso‐Chávez, Ricardo; Ruíz-Valdiviezo, Víctor Manuel

doi:10.1007/s00284-023-03327-8

Cited by 1 publication

(1 citation statement)

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“…Temperatures in the lake vary between 20 °C and 95 °C, and the pH varies between 2 and 4. The crater lake has been the source of extremophile bacteria (Ovando- Frontiers in Systems Biology frontiersin.org Chacon et al, 2020;Ortiz-Cortés et al, 2021;Ovando-Ovando et al, 2023), and recently proteobacterial methanotrophs were identified in the sediments (Rincón-Molina et al, 2019;Rincón-Molina et al, 2020). Supplementary Table S1 contains the coordinates of the different sites.…”

Section: Sample Collectionmentioning

confidence: 99%

Genome-scale flux balance analysis reveals redox trade-offs in the metabolism of the thermoacidophile Methylacidiphilum fumariolicum under auto-, hetero-and methanotrophic conditions

Saldivar,

Ruiz-Ruiz,

Revah

et al. 2024

Front. Syst. Biol.

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Members of the genus Methylacidiphilum are thermoacidophile methanotrophs with optimal growth temperatures between 50°C and 60°C, and pH between 1.0 and 3.0. These microorganisms, as well as other extremophile bacteria, offer an attractive platform for environmental and industrial biotechnology because of their robust operating conditions and capacity to grow using low-cost substrates. In this study, we isolated Methylacidiphilum fumariolicum str. Pic from a crater lake located in the state of Chiapas, Mexico. We sequenced the genome and built a genome-scale metabolic model. The manually curated model contains 667 metabolites, 729 reactions, and 473 genes. Predicted flux distributions using flux balance analysis identified changes in redox trade-offs under methanotrophic and autotrophic conditions (H2+CO2). This was also predicted under heterotrophic conditions (acetone, isopropanol, and propane). Model validation was performed by testing the capacity of the strains to grow using four substrates: CH4, acetone, isopropanol, and LP-Gas. The results suggest that the metabolism of M. fumariolicum str. Pic is limited by the regeneration of redox equivalents such as NAD(P)H and reduced cytochromes.

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