Isolation and identification of bacteria with capability of starch, cellulose and protein decomposition from Frass of Black Soldier Fly (Hermetia Illucens) Larvae

Abstract

This research isolated and identified bacteria with capability of starch, cellulose and protein decomposition from frass of black soldier fly (Hermetia Illucens) larvae (BFL). Dilute suspension of BFL frass was spread on petri dishes with solid Nutrient Agar for bacteria isolation. Selection and evaluation of bacteria with capability of starch, cellulose and protein decomposition was based on their ability of creating halo zone on starch agar, CMC and skim milk media. Identification of selected bacterial isolates was based on 16S rDNA sequences. There were 44 bacterial strains isolated from BSFL frass. Bacterial strains with the best capability of starch, cellulose and protein decomposition were CCRL21 and CCRL16 with halo zone radii of 1.5 cm and 0.8 cm; CCRL16, CCRL20 with halo zone radii with the similar size of 0.83 cm; and CCRL44 has the best ability of protein decomposition with an average halo zone radius about 2.47 cm, respectively. Six bacterial strains with good ability of degrading cellulose, starch and protein were identified as Bacillus paramycoides CCRL1; Bacillus altitudinis CCRL16, Bacillus spizizenii CCRL20, Bacillus altitudinis CCRL21, Lysinibacillus pakistanensis CCRL32 and Paenibacillus silvae CCRL43.