ISOLATION AND IDENTIFICATION OF BACTERIA FROM CELL PHONES, LAPTOP AND BIOMETRIC MACHINE OF GOVERNMENT MEDICAL COLLEGE JAMMU, (J & K), INDIA

Abstract

Background: Globally, the COVID-19 coronavirus pandemic epidemic spread quickly. The assessment of illness severity may be made easier by taking a variety of laboratory markers and comorbidities into account. For effective patient triaging, it is crucial to identify disease progression early on in severe COVID19 cases. In the course and severity of COVID-19 patients, our study examined the features, function, and significance of laboratory findings and comorbidities. Methods: From early june to midaugust 2021, the study was carried out. At District hospital kulgam, jammu and kashmir, 31 patients were given blood samples and clinical information. In this investigation, biological indicators such as total blood count were used. Blood sugar, serum ferritin, erythrocyte sedimentation rate (ESR), (CBC), D-dimer, and C-reactive protein(CRP) protein and SpO2. The present study showed that all mobile phones, laptops and biometric machines in office and college were infected by several microbes, most of which belonged to the natural flora of the human body. This means that it is necessary to sterilize hands after contact with a phone, laptop and most important biometric machines since it is a main source of disease transmission Total 110 device was used for this study including cell phone and laptop of Government Medical College (G.M.C) workers with his permission and biometric machines with the permission of Head of G.M.C Jammu. In this study the results show that isolated bacterial genus are gram positive Bacillus ,Staphylococcus, Streptococcus and gram negative E.coli and Klebsiella isolated from the cell phones ,laptop and biometric machines. Bacillus and E coli Records the highest percentages (9%) among other Gram-positive and Gram negative isolates on the laptop keyboards, cell phone and biometric machine respectively, followed by Staphylococcus aureus (7.2 %), Streptococcus spp. (6%).The bacterium Escherichia coli hold the first rank among other Gram-negative bacteria; it had been recorded (9 %), while the bacterium Klebsiella pneumoniae recorded (4.8 %).After isolation of bacteria in selective agar media it was purified in blood agar and MacConkey agar. Gram staining of each plate was identified bacteria morphologically 15 was gram positive bacillus, 10 was Streptococcus, 12 was Staphylococcus and in gram negative 15 was E coli and 8 Klebsiella. For further confirmation of bacterial genus biochemical analysis was done for 60 bacterial isolates, all isolates gives negative result for oxidase. Except Streptococcai all isolates show positive result for catalase.. For clarification between the of E coli and Klebsiella,two test was performed ,one is motility and second is indole. E coli always are motile and indole positive but Klebsiella gives negative result for both. Sixty bacterial isolates were subjected to susceptibility testing. Overall, isolates showed high level of resistance to ampicillin (91.7%) and tetracycline (72.8%), a moderate resistance to chloramphenicol (42.5%), and a lower resistance to cefotaxime (...