Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR

Ali, Shahzad; Ali, Qurban; Melzer, Falk; Khan, Iahtasham; Akhter, Shamim; Neubauer, Heinrich; Jamal, Syed Muhammad

doi:10.1007/s11250-013-0448-6

Cited by 40 publications

(40 citation statements)

References 12 publications

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“…Recently, Ali et al (2014) described the isolation of 30 B. abortus strains in aborted foeti, vaginal swabs and milk samples collected from bovids that had either aborted or produced antibodies to Brucella in milk. Strains were confirmed as B. abortus by cultural and molecular methods (Bricker and Halling, 1994).…”

Section: Comparison Of Culture Serological and Molecular Diagnostic mentioning

confidence: 99%

Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella melitensis Strains Isolated from Pakistan

Mahmood¹,

Waheed²,

Ali³

et al. 2016

IJAB

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This study aimed at molecular detection and characterization of Brucella spp from Pakistan. For this purpose, whole blood samples (n=167) were collected from different species of livestock and analysed at Animal and Plant Health Agency (APHA), United Kingdom. Samples were analysed employing Rose Bengal Test (RBT), Competitive Enzyme-linked immunosorbent assay (cELISA), PCR IS711 and culture examination. We found 1% (2/167) samples positive for infection by culture, 4% (7/167) by RBT, 6% (10/167) by cELISA and 21% (35/167) by PCR IS711. Results were found statistically significant using chi-square test (p-value <0.05). The blood culture positive bacterial isolations were further subjected to classical biotyping and molecular techniques for characterization and found as non-vaccine strains of Brucella melitensis. Molecular characterization using Multilocus Sequence Analysis (MLSA) and Variable Number of Tandem Repeat (VNTR) analysis demonstrated that although there was some similarity in the patterns generated, the isolations were distinct from each other. These isolates were not the part of geographically confined group but were representative of other B. melitensis strains found in the region stretching from southern Europe into South Asia. To our knowledge, this is the first report of molecular characterization of B. melitensis isolates from Pakistan. The molecular methods described in the present study will help to understand the disease dynamics and future brucellosis control in Pakistan.

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Section: Comparison Of Culture Serological and Molecular Diagnostic mentioning

confidence: 99%

Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella melitensis Strains Isolated from Pakistan

Mahmood¹,

Waheed²,

Ali³

et al. 2016

IJAB

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“…Brucellosis is an important zoonotic disease that affects a wide range of animal species as well as humans [1,2]. The causative agent of brucellosis in goats and sheep is Brucella melitensis, while B. abortus, B. ovis, and B. suis may cause infections only under certain condition [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…Among the different livestock species in Pakistan are 28.1 million sheep and 61.5 million goats [8]. B. abortus biotype 1 is known to be the causative agent of brucellosis in cattle and buffaloes [2]. Quantitative real-time polymerase chain reaction (qRT-PCR) assays have confirmed B. abortus as the causative agent of human brucellosis in high-risk occupations in Pakistan [1].…”

Section: Introductionmentioning

confidence: 99%

Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan

Ali

Akhter

Neubauer

et al. 2015

J Infect Dev Ctries

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Introduction: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan. Methodology: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT). Seropositive samples were subjected to bacterial isolation and PCR analysis using Brucella genus-specific (bcsp31) and Brucella species-specific (IS711 for Brucella abortus and Brucella melitensis) quantitative real-time polymerase chain reactions (qRT-PCR). Results: Twenty-four (8.6%) serum samples were positive by RBPT. Twenty (9.4%) animals were positive for Brucella antibodies using MRT. No Brucella isolates were obtained from the examined blood and milk samples. Of the 24 seropositive serum samples, 18 (75%) were positive in the Brucella genus-specific (bcsp31) and Brucella abortus-specific (IS711) qRT-PCR, respectively. Conclusions: Brucella abortus was identified as causative agent of ovine and caprine brucellosis in Pakistan. Results of this study can be used for the development of an effective control and eradication strategy for brucellosis in livestock, especially small ruminants.

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“…They are providers of nourishment for billions of rural and urban households as well as income and employment for producers and others working in simple and sometimes complex value chains. Large ruminants, especially domestic water buffalo (Bubalus bubalis) are an important livestock species worldwide as it is a source of high quality milk and meat, leather and traction power, in addition to being able to survive on marginal land and subsist on low-quality forages, a good alternative for the breeding livestock especially in tropical countries [1][2][3]. Water buffalo is known for its rusticity and great adaptability to different topographies, soils and climatic factors.…”

Section: Introductionmentioning

confidence: 99%

Emerging Infectious Diseases in Water Buffalo: An Economic and Public Health Concern

Villanueva¹,

Mingala²,

Tubalinal³

et al. 2018

Emerging Infectious Diseases in Water Buffalo - An Economic and Public Health Concern

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Water buffalo is an indispensable livestock in Asia and other countries due to its high meat and milk quality, aside from draft power source. It adapts well to tropical climate and has significant contribution to the livestock industry, provided with improved breeding and good animal husbandry practices. Infectious diseases are hindrance to good reproductive performance of livestock, resulting in huge economic loss. In addition, most of these diseases are zoonotic, posing serious threats on human health. However, its degree of severity varies in each region and is often overlooked. This chapter reviews the common and current updates on emerging bacterial, viral, protozoal, fungal and endoparasitic pathogens that infect water buffaloes worldwide. All of the diseases directly affect the animals' health condition except for schistosomiasis where water buffalo played an important role as shedder of infection to humans. Leptospirosis, brucellosis, Bovine Tb, BVDV and fasciolosis have projected economic impact to water buffalo industry as well as its effect as zoonoses. However, the data seem underquantified since most are neglected diseases and are highly prevalent in developing countries. Further studies are needed particularly in countries where water buffalo is the major livestock than cattle to fully utilize the potential of the animal.

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Isolation and identification of bovine Brucella isolates from Pakistan by biochemical tests and PCR

Cited by 40 publications

References 12 publications

Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella melitensis Strains Isolated from Pakistan

Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella melitensis Strains Isolated from Pakistan

Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan

Emerging Infectious Diseases in Water Buffalo: An Economic and Public Health Concern

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