Isolation and Identification of Cellulose Degrading Bacteria from Different Sources at Assiut Governorate (Upper Egypt)

Rawway, Mohammed; Ali, Salah G.; Badawy, Ahmed S.

doi:10.18576/jehe/060103

Cited by 36 publications

(25 citation statements)

References 29 publications

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“…The isolates then cultivate in 20 mL of 1% CMC-broth medium as much as 20 μL with equal cell density was 10 7 cells.mL -1 was taken and inoculated on blank disc paper and placed onto 1% CMC-agar medium and incubated at 30 °C for 72 hours. After incubation was completed, then flooded with 1% Congo Red solution for 15 minutes and it washed with 1M NaCl solution [12]. The cellulolytic index (CI) was determined with the formula 1.…”

Section: Isolation and Screening Of Cellulolytic Bacteriamentioning

confidence: 99%

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Faizah

Ardyati

Suharjono

2020

JELS

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Palopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulolytic bacteria from sago pith waste as a biodegradation agent. Bacteria were isolated from sago pith waste and grown on a 1% Carboxyl methyl cellulose (CMC) agar medium. The cellulolytic activity was analyzed semiquantitatively using 1% Congo red and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH variations of 4, 5, and 6. The potential isolate was identified based on 16S rDNA sequence similarity. This study obtained 21 bacterial isolates where six isolates were A1D, A1E, A1I, A1K, A2A, and B1A had the highest cellulolytic index at 0.82-1.13. Among those six isolates, the A1E isolate had the highest cellulolytic activity, 0.54 U.mL-1 at pH 6. The isolate A1E was identified as Burkholderia cepacia JCM 2799 with 99.73% similarity of 16S rDNA sequence.

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Section: Isolation and Screening Of Cellulolytic Bacteriamentioning

confidence: 99%

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Faizah

Ardyati

Suharjono

2020

JELS

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“…These classes of enzymes are endoglucanases, exoglucanases, and β-glucosidases. In nature, cellulose degrading bacteria (e.g., Clostridium, Cellulomonas, Thermonospora, Bacillus, Bacteriodes, Ruminococcus, Erwinia, Acetovibrio, Microbispora, Streptomyces) have been isolated from different sources such as gut of termites, snail, bookworm, caterpillar (Gupta et al, 2011(Gupta et al, , 2012Gupta and Verma, 2015), ruminants (bovine rum), soil samples (including deep subsurface soil), and human excreta (Robert and Bernalier-Donadille, 2003;Rastogi et al, 2009;Rawway et al, 2018). Lots of works in the past, since 1950s (Hungate, 1950), have been done to convert lignocellulosic biomass into bioethanol efficiently, but with little success (Gupta et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues

et al. 2019

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In the present study, 11 cellulose degrading bacterial strains were isolated from water and soil samples of hot springs in the Chumathang village, Leh and Ladakh region, India. The isolated strains were identified as Bacillus subtilis, Bacillus aryabhattai, Bacillus stratosphericus, Bacillus altitudinis, and Brevibacterium frigoritolerans by biochemical and molecular approaches. All the strains were evaluated for the total cellulase, endoglucanase, exoglucanase, and β-glucosidase enzyme activities. On the basis of overall individual cellulose degrading enzyme activities, three strains were selected to develop consortium to enhance their cellulase enzyme activities. The potent cellulose degraders B. stratosphericus BHUJPV-H5, B. subtilis BHUJPV-H19, and B. subtilis BHUJPV-H12 were selected for the consortium development. The effect of cellulase activities of bacterial strains were evaluated ranged up to 6.06 and 0.72 mg ml −1 glucose by agro-residues of sugarcane bagasse and wheat straw, respectively, after 1 h of incubation. Total cellulase enzyme activity of consortia was recorded two times higher than the individual organisms. These strains can be used for enhancing bioethanol production from lignocellulosic biomass, which can consequently boost biofuel production industry. HIGHLIGHTS-Bacillus strains were isolated from hot spring's water and soil samples.-Total cellulase enzyme activity was two times higher in the form of consortium.β-glucosidase activity was found to be high, with maximum 5.65 Uml −1 .-Use of wheat straw and sugarcane bagasse showed good amounts of glucose release on hydrolysis.

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“…The plates were soaked with Gram's iodine following incubation for 48 h at room temperature (28 ±2 ˚C). Finally, bacterial isolates with the highest mean diameter of clear zones were selected for further studies (Rawway, Ali & Badawy, 2018).…”

Section: Primary Screening Of Cellulase Producing Bacteriamentioning

confidence: 99%

Isolation and Identification of Cellulase Producing and Sugar Fermenting Bacteria for Second-Generation Bioethanol Production

Weerasinghe

Madusanka

Manage

2021

Int. J. Renew. Energy Dev.

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Over the last decades, the negative impacts of fossil fuel on the environment and increasing demand for energy due to the unavoidable depletion of fossil fuels, has transformed the world’s interests towards alternative fuels. In particular, bioethanol production from cellulosic biomass for the transportation sector has been incrementing since the last decade. The bacterial pathway for bioethanol production is a relatively novel concept and the present study focused on the isolation of potential “cellulase-producing” bacteria from cow dung, compost soil, and termite gut and isolating sugar fermenting bacteria from palm wine. To select potential candidates for cellulase enzyme production, primary and secondary assays were conducted using the Gram’s iodine stain in Carboxy Methyl Cellulose (CMC) medium and the Dinitrosalicylic acid (DNS) assays, respectively. Durham tube assay and Solid-Phase Micro-Extraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS) was used to evaluate the sugar fermenting efficiency of the isolated bacteria. Out of 48 bacterial isolates, 27 showed cellulase activity where Nocardiopsis sp. (S-6) demonstrated the highest extracellular crude enzyme activity of endoglucanase (1.56±0.021 U) and total cellulase activity (0.93±0.012 U). The second-highest extracellular crude enzyme activity of endoglucanase (0.21±0.021 U) and total cellulase activity (0.35±0.021 U) was recorded by Bacillus sp. (T-4). Out of a total of 8 bacterial isolates, Achromobacter sp. (PW-7) was positive for sugar fermentation resulting in 3.07% of ethanol in broth medium at 48 h incubation. The results of the study revealed that Nocardiopsis sp. (S-6) had the highest cellulase enzyme activity. However, the highest ethanol percentage was achieved with by having both Bacillus sp. (T-4) and Achromobacter sp. (PW-7) for the simultaneous saccharification and fermentation (SSF) method, as compared to separate hydrolysis and fermentation (SHF) methodologies.

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Isolation and Identification of Cellulose Degrading Bacteria from Different Sources at Assiut Governorate (Upper Egypt)

Cited by 36 publications

References 29 publications

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues

Isolation and Identification of Cellulase Producing and Sugar Fermenting Bacteria for Second-Generation Bioethanol Production

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