Isolation and Identification of Dominant N2 Fixing Cyanobacterial Strains from Different Locations

Abstract

Isolation of cyanobacteria dominated in the soil samples collected from different sites in Kafr El-Sheikh, and El-Dkahlia governorates isolates were successfully obtained as bacterial free cyanobacteria. They were identified cyanobacteria was carried out using the following: thallus color, thallus morphology and size of heterocyst, vegetative and reproductive cells. Heterocyst-forming cyanobacteria were also cultured as Anabaena sp., Nostoc sp., Oscillatoria sp., and Chroococcus sp. Nostoc sp . were isolated at high frequency; Nostoc sp. and Anabaena sp. isolates representing in kafr El-Sheikh and El-Dakahlia Governorates. Less frequency of cyanobacteria belonging to different genera namely Oscillatoria sp. and Chroococcus sp. Nostoc spp.were recorded highest amount of fixed nitrogen and mass production with in increasing incubation period of interacellular and /or extracellular nitrogenfixation. While,the most active strains for nitrogenase activity were Nostoc mucorum K and Anabaena oryzae D.

Inoculation with Single, Dual or Consortia of Rhizobium leguminosarum bv. trifolli , Pseudomonas stutzeri and Anabaena sp. and their effect on Yield Components of Rice Plant

“…Previous field inoculation studies on rice have used N 2 -fixing cyanobacteria, Azospirillum, Azotobacter and other candidates as biofertilizer inoculants ( Omar et al 1993, Shahaby et al 1993and Yanni and Abd El-Fattah 1999& Afify et al, 2018.They concluded that inoculation with cyanobacteria positively contributed to rice plant growth, paddy yield and grain quality, with positive turnover effects on soil fertility and productivity of next crop(s). However, this is also confirmed in this study which clearly documenting similar positive effects of inoculation with Nfixing cyanobacteria ( Abou -Elatta , 2018 ) .…”

“…Several cyanobacterial strains belonging to Nostoc and Anabaena of the rhizosphere isolates of cyanobacteria, which were found efficient in nitrogen fixation (Prasanna et al, 2009 andAfify et al, 2018).…”

Factors Affecting Biodegradation of the Insecticides by Cyanobacterial Strains, Isolated from Soil Contaminated with Pesticides

“…Some chemical characteristics of chlorpyrifos according to Venkta Mohan et al, (2004). Also, chemical characteristics of carbofuran according to Chowdhury et al (2014) are presented in Abou Elatta et al, (2023) and Afify et al, (2023b). These insecticides were obtained from Sigma -Co., USA.…”

Use of Cyanobacteria for Controlling Flax Seedling Blight

“…The five cyanobacterial strains " Nostoc muscorum k , Anabaena oryzae D, Anabaena oryzae k , Nostoc pruniforme D , and Oscillatoria brevis D " were isolated from rhizosphere of rice (Oryzae sativum L. ) plant , cyanobacterial strains were identified morphologically , biochemically , and by cultural characters as described previously (Afify et al , 2018) .These five cyanobacterial strains are chosen from fourteen strains because they were the most antagonistic ones against the flax pathogenic fungi F.oxysporum and R. solani . All cyanobacterial strains were isolated from rhizosphere of rice plant in the governorates of El-Dakahliya (D) and kafr El -Sheikh (k).…”