Isolation and Identification of Encapsulated Strains of Bacteroides fragilis

Kasper, Dennis L.; Hayes, Marie E.; Reinap, Barbara; Craft, Floyd O.; Onderdonk, Andrew B.; Polk, B. Frank

doi:10.1093/infdis/136.1.75

Cited by 95 publications

(35 citation statements)

References 16 publications

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“…An EDL of approximately 35 nm has been observed outside the OM in clinical isolates of B. fragilis and some strains of Bacteroides thetaiotaomicron and Bacteroides vulgatus by EM after RR staining (Kasper et al, , 1979. Similar structures have been reported by Lindberg et al (1979) andBrook et al (1984), but none of these authors reported the presence of fibrous material, Babb & Cummins (1 977) observed capsules one to four times the diameter of the cell by negative stain and LM.…”

Section: S P a T R I C K J H R E I D A N D A C O F F E Y Disupporting

confidence: 60%

“…Similar structures have been reported by Lindberg et al (1979) andBrook et al (1984), but none of these authors reported the presence of fibrous material, Babb & Cummins (1 977) observed capsules one to four times the diameter of the cell by negative stain and LM. These authors considered it likely that this structure differed from the RR stained layer described by Kasper et al (1977). These anomalies could be due to differences in the composition of the culture media (Patrick & Reid, 1983).…”

Section: S P a T R I C K J H R E I D A N D A C O F F E Y Dimentioning

confidence: 98%

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Capsulation of in vitro and in vivo Grown Bacteroides Species

1986

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Section: S P a T R I C K J H R E I D A N D A C O F F E Y Disupporting

confidence: 60%

Section: S P a T R I C K J H R E I D A N D A C O F F E Y Dimentioning

confidence: 98%

Capsulation of in vitro and in vivo Grown Bacteroides Species

1986

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“…Romond et al (1972) showed that 74 % of 58 presumably pathogenic strains of B. fragilis were agglutinated by two of their six specific 0-antisera (E1, E2 or El E2) raised against two strains of B.fragilisfragilis. Kasper et al (1977), on the other hand demonstrated that most strains ofB.fragilis fragilis were encapsulated and that the capsular polysaccharide derived from these strains constituted the virulence factor . Furthermore, strains lacking this capsule are less capable of promoting infection .…”

Section: Discussionmentioning

confidence: 99%

“…This virulence factor may be related to its encapsulation (Polk & ; and more specifically to its capsular polysaccharide . Antisera raised against this capsular polysaccharide reacted with almost all clinical strains of B. fragilis fragilis but not with other subspecies (Polk & Kasper, 1977).…”

Section: Introductionmentioning

confidence: 93%

The distribution ofBacteroides fragilisserotypes amongst clinical strains

Elhag

Tabaqchali

1978

J. Hyg.

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Three hundred and twenty-two strains ofBacteroidesfragilis isolatedfrom infected patients at three different hospitals were tested against 20 type specific B. fragilis antisera using the tube agglutination technique. Of these strains 41'3 % were assigned to a single 0-serotype, a further 20-5 % were agglutinated by several antisera and could not be classified and the remainder showed no reactions. Three different serotypes were prevalent in the three hospitals and minor geographical variation was observed.No correlation was found between serotypes and the origin of infection, but those from the blood were the most readily typable strains. No correlation was found between serotypes and biotypes of B. fragilis.

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“…In a rat model simulating human intraabdominal sepsis, B. fragilis, but not other anaerobic bacterial species, has the distinct ability to induce abscesses when implanted into the peritoneal cavities of animals (11)(12)(13)(14). Anaerobic species other than B. fragilis require co-administration with a facultative organism to induce abscesses.…”

mentioning

confidence: 99%

T Cells Activated by Zwitterionic Molecules Prevent Abscesses Induced by Pathogenic Bacteria

Tzianabos¹,

Finberg²,

Wang³

et al. 2000

Journal of Biological Chemistry

102

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Immunologic paradigms classify bacterial polysaccharides as T cell-independent antigens. However, these models fail to explain how zwitterionic polysaccharides (Zps) confer protection against intraabdominal abscess formation in a T cell-dependent manner. Here, we demonstrate that Zps elicit a potent CD4؉ T cell response in vitro that requires available major histocompatibility complex class II molecules on antigen-presenting cells. Specific chemical modifications to Zps show that: 1) the activity is specific for carbohydrate structure, and 2) the proliferative response depends upon free amino and carboxyl groups on the repeating units of these polysaccharides. Peptides synthesized to mimic the zwitterionic charge motif associated with Zps also exhibited these biologic properties. Lysine-aspartic acid (KD) peptides with more than 15 repeating units stimulated CD4؉ T cells in vitro and conferred protection against abscesses induced by bacteria such as Bacteroides fragilis and Staphylococcus aureus. Evidence for the biologic importance of T cell activation by these zwitterionic polymers was provided when human CD4؉ T cells stimulated with these molecules in vitro and adoptively transferred to rats in vivo conferred protection against intraabdominal abscesses induced by viable bacterial challenge. These studies demonstrate that bacterial polysaccharides with a distinct charge motif activate T cells and that this activity confers immunity to a distinct pathologic response to bacterial infection.The study of T cell recognition of foreign antigens has been directed primarily toward an understanding of the host's immune system response to proteins or peptides. The recent demonstration that non-peptide-containing mycobacterial lipid and glycolipid antigens activate T cells in conjunction with CD1 molecules on the surface of antigen-presenting cells (APCs)

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Isolation and Identification of Encapsulated Strains of Bacteroides fragilis

Cited by 95 publications

References 16 publications

Capsulation of in vitro and in vivo Grown Bacteroides Species

Capsulation of in vitro and in vivo Grown Bacteroides Species

The distribution ofBacteroides fragilisserotypes amongst clinical strains

T Cells Activated by Zwitterionic Molecules Prevent Abscesses Induced by Pathogenic Bacteria

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