Isolation and Identification of Helicase from Escherichia coli for Biotechnology Processes

Sasidharan, Sreenivasan; Yamuna, S.; Eugene, O.; Venugopal, Balaji; Asma, I.

doi:10.1016/j.apcbee.2012.06.030

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References 9 publications

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“…These results indicate that there is a chemical variability in the accessions essential oil studied, highlighting the landraces compared to cultivated accessions. HE et al, 1998;RAI et al, 2006;YUSUFOGLU; ALQASOUMI, 2010) e do óleo essencial, cuja composição é analisada, sobretudo, por cromatografia gasosa acoplada a espectrômetro de massas (CG-SM) (ONYENEKWE;HASHIMOTO, 1999;WOHLMUTH et al, 2006;WANG et al, 2006;ARAGAW et al, 2011;HASSANPOURAGHDAM et al, 2011;PADALIA et al, 2011;SASIDHARAN;VENUGOPAL;MENON, 2012;DING et al, 2012;PANDOTRA, P et al, 2013;SINGH et al, 2013). Em uma pesquisa na Índia conduzida por Kizhakkayil e Sasikumar (2009), a caracterização de 46 acessos de gengibre revelou que os materiais primitivos (cultivares locais) "Sabarimala", "Kozhikkalan" e "Kakakalan" foram mais ricos em oleoresina, teor de óleo essencial e fibra crua, quando comparados com as cultivares melhoradas.…”

Section: Introductionunclassified

Diversidade genética e química em germoplasma de gengibre (Zingiber officinale)

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Chemical and genetic diversity of ginger germplasm (Zingiber officinale) Plant genetic resources exhibit a true and potential value for agriculture due to their importance in breeding and conservation programs. This study aimed to characterize the genetic and chemistry diversity of ginger germplasm of Genetics Department of the ESALQ/USP, through 18 phenotypic descriptors, 13 AFLP markers combinations and chemical composition analysis of essential oil. The germplasm was combined by accessions coming from diferent Brazilian states, along with some accessions introduced from Colombia. During the collection, 34 farmers were interviewed in three states: São Paulo (SP), Espírito Santo (ES) and Paraná (PR). Ginger is mainly cultivated by small farmers whose main income source is agriculture. In the chemical composition analysis, a total of 61 compounds were identified. The accessions presented chemical variability according to the analysis of variance. The essential oil was rich in monoterpenes (82.35%), being the geranial (20.41%) and the neral (13.36%), both referred to as citral, the most abundant compounds. The α-zingiberene, 1,8-cineole, linalool and β-phellandrene compounds were relevant in the chemical diversity analysis of the accessions, while the dendrogram based on the complete linkage method (LC-VD) classified the germplasm into two groups: landraces and cultivars accessions. In the agromorphologic characterization, qualitative traits showed little variation, while moderate to low variability was observed for quantitative traits, although some divergent accessions were identified. The principal component analysis explained 82% of the total variation in the first three components, wherein the accessions distribution on the scatter plot was consistent with the groups formed by the Tocher method, with the Gen-18, Gen-24, Gen-65 and Gen-42 accessions as the most divergent ones from the phenotypically germplasm. In the molecular characterization, 13 AFLP markers combinations generated an average of 113.5 polymorphic loci, with a ratio of 96.85% of polymorphism in the overall collection. Estimates of Nei genetic diversity (H j), Shannon index (I) and alleles effective number (n e) were higher in Colombian accessions (0.501; 0.396 and 1.508, respectively). The AMOVA showed that most of the variation (63%) occurs between the two countries and the F ST =0.153 index suportted this result, indicating high genetic differentiation between Brazilian and Colombian accessions. The groupings provided by Structure and the dendrogram based on Jaccard coefficient complement were consistent with each other and showed that Brazilian accessions are genetically similar. In general, there is no influence of the accesses geographic origin in the chemical, phenotypic and molecular grouping pattern. The introduction of new genetic materials in Brazil, will certainly contribute to a broader genetic basis of this species.

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Section: Introductionunclassified

Diversidade genética e química em germoplasma de gengibre (Zingiber officinale)

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Isolation and Identification of Helicase from Escherichia coli for Biotechnology Processes

Cited by 1 publication

References 9 publications

Diversidade genética e química em germoplasma de gengibre (Zingiber officinale)

Diversidade genética e química em germoplasma de gengibre (Zingiber officinale)

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