Isolation and identification of hydrocarbons degrading bacteria in soil around Shiraz Refinery

Abstract

Petroleum hydrocarbons are species of dangerous contaminants in nature. Scientists are looking for many years to find solution for removing contaminants from the soil and water environments. Today, the use of microorganism, for removing crude oil pollution from contaminated sites (bioremediation) is considered by scientists. The purpose of this research is to isolate and identify hydrocarbons degrading bacteria from Shiraz Refinery soil. In this study, nutrient agar (NA) and mineral salt agar (MM2) were used as basic cultures. Using the Techniques Replica plating, we transferred colonies on NA surface to the MM2 agar. For spraying hydrocarbons on the MM2 agar surface, we used spray plating technique. Finally we have identified different bacterial genera by using biochemical tests and morphological study. Upon examination on morphological studies and biochemical tests, it is determined that these strains belong to bacterial genera as follows: Bacillus, Corynebacterium,

Prenatal exposure to polycyclic aromatic hydrocarbons and effects on neonatal anthropometric indices and thyroid-stimulating hormone in a Middle Eastern population

“…In Shiraz, about 90 % of trips are made by car, which can be considered one reason for increasing this pollutant's concentration (Kamrannia, 2016). The results of the conducted studies in Shiraz illustrated the high concentrations of PAHs in the air (Shahsavani et al, 2017(Shahsavani et al, , 2019, road dust (heavy traffic) (Soltani et al, 2015), soil (industrial activities) (Kafilzadeh et al, 2011a) and water and sediments (Kafilzadeh, 2015;Kafilzadeh et al, 2011b).…”

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

“…The genus Acinetobacter sp. has been reported in literature as a PAHs degrader (Yu et al, 2005; Janbandhu and Fulekar, 2011; Kafilzadeh et al, 2011). Kostka et al (2011) isolated 24 bacterial strains belonging to 14 different genera, including Acinetobacter, from beach sand affected by oil discharge.…”

Comparing the Biodegradability of Petroleum-based Plastic with a Novel, Sustainable Bio-plastic Alternative

“…The product's ability to biodegrade is mainly due to the utilization of organic constituents in the formula (20). The unnatural bonding in polyethylene is responsible for its indestructible nature, as few microorganisms are equipped with the metabolic pathways needed to weaken the long chains of such interactions (21). Evidence of this is seen in the FTIR spectra between 1400 and 1600 cm -1 (region III), where a peak indicating carbon-carbon double bonding is visible for polyethylene (22).…”