Isolation and Identification of
            <i>Bifidobacteriaceae</i>
            from Human Saliva

Beighton, David; Gilbert, Steven C.; Clark, Douglas; Mantzourani, Maria; Al-Haboubi, Mustafa; Ali, Farida; Ransome, Elizabeth; Hodson, Nicholas Anthony; Fenlon, Michael R.; Zoitopoulos, L; Gallagher, Jennifer E.

doi:10.1128/aem.00895-08

Cited by 51 publications

(68 citation statements)

References 35 publications

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“…The current study corroborates the finding of the study of Becker et al, which further clarifies its association with dentinal caries, although S. wiggsiae was also detected in early, white spot lesions (3). Bifidobacteriaceae are generally acidogenic and acid tolerant and have been significantly associated with caries in adults by culture (5,34,53) and in children (1,30) and in our studies with severe ECC using PCR (36). In the current study, the combination of S. mutans and S. wiggsiae was highly associated with severe ECC.…”

Section: Vol 49 2011 Culture Of Early Childhood Caries 1471supporting

confidence: 92%

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Cultivable Anaerobic Microbiota of Severe Early Childhood Caries

Mathney²,

et al. 2011

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Severe early childhood caries (ECC), while strongly associated with Streptococcus mutans using selective detection (culture, PCR), has also been associated with a widely diverse microbiota using molecular cloning approaches. The aim of this study was to evaluate the microbiota of severe ECC using anaerobic culture. The microbial composition of dental plaque from 42 severe ECC children was compared with that of 40 caries-free children. Bacterial samples were cultured anaerobically on blood and acid (pH 5) agars. Isolates were purified, and partial sequences for the 16S rRNA gene were obtained from 5,608 isolates. Sequence-based analysis of the 16S rRNA isolate libraries from blood and acid agars of severe ECC and caries-free children had >90% population coverage, with greater diversity occurring in the blood isolate library. Isolate sequences were compared with taxon sequences in the Human Oral Microbiome Database (HOMD), and 198 HOMD taxa were identified, including 45 previously uncultivated taxa, 29 extended HOMD taxa, and 45 potential novel groups. The major species associated with severe ECC included Streptococcus mutans, Scardovia wiggsiae, Veillonella parvula, Streptococcus cristatus, and Actinomyces gerensceriae. S. wiggsiae was significantly associated with severe ECC children in the presence and absence of S. mutans detection. We conclude that anaerobic culture detected as wide a diversity of species in ECC as that observed using cloning approaches. Culture coupled with 16S rRNA identification identified over 74 isolates for human oral taxa without previously cultivated representatives. The major caries-associated species were S. mutans and S. wiggsiae, the latter of which is a candidate as a newly recognized caries pathogen.

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Section: Vol 49 2011 Culture Of Early Childhood Caries 1471supporting

confidence: 92%

“…B. dentium was the major species detected from childhood caries (30) using a selective medium for intestinal bifidobacteria modified for oral samples (5). Another unnamed Bifidobacterium species, HOT 198, was detected in one caries-free child (Fig.…”

Section: Bifidobacteriaceae Lactobacillus and Actinomyces Speciesmentioning

confidence: 99%

Cultivable Anaerobic Microbiota of Severe Early Childhood Caries

Mathney²,

et al. 2011

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“…The available significant rRNA gene sequencing studies pertaining to the oral microbiome is shown in Quantity of these organisms are highly associated with higher number of dental caries associated bacteria (Beighton et al, 2008) (…”

Section: Metagenomic Detection Of the Oral Microbiomementioning

confidence: 99%

Exploring the oral bacterial flora: current status and future directions

et al. 2010

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Oral Diseases (2010) 16, 136–145 Objective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches.

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“…(6,7) Again, although encouraging results have been observed, most studies have been fairly short. Furthermore, in some studies the observed differences were quite small, though statistically significant.…”

mentioning

confidence: 99%