Isolation and identification of iturins as antifungal peptides in biological control of peach brown rot with Bacillus subtilis

Gueldner, R. C.; Reilly, Charles C.; Pusey, P.L.; Costello, Catherine E.; Arrendale, Richard F.; Cox, Richard H.; Himmelsbach, David S.; Crumley, Farrist G.; Cutler, Horace G.

doi:10.1021/jf00080a031

Cited by 108 publications

(46 citation statements)

References 13 publications

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“…The equipment was a SHIMADZU LC-10A coupled to a detector SPD-10AV. Procedures were as those previously reported (Schreiber et al 1988;Gueldner et al 1988).…”

Section: Reverse Liquid Chromatography Of the Antibiotic Metabolitementioning

confidence: 99%

Genomic variability in Vitis vinifera L. Sangiovese assessed by microsatellite and non-radioactive AFLP test

Vignani¹,

Scali²,

Masi³

et al. 2002

Electron. J. Biotechnol.

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The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. There are many examples of formulations using bacterial or fungal strains with biocontrol applications. Among them, members of the genus Bacillus are well known antibiotic producers. However, the increased capacity of antibiotic production obtained by direct mutagenesis of wild strains, has seldom been reported in the open literature. This research refers to the mutation of the A47 Bacillus strain, a plant pathogen antagonist, in order to obtain an improved strain with enhanced capacity to synthesize metabolites with antibiotic activity. The mutant strain M40 was obtained using the mutagenic agent acridine orange. The mutant strain showed a higher antagonistic activity than the wild type A47 against the plant pathogen Botritys cinerea (grey mould), Ralstonia solanacearum (bacterial wilt) and Erwinia carotovora var. carotovora (bacterial soft rot). The final objective was to isolate the antibiotic metabolite produced by the M40 strain and to determine its chemical and antibiotic properties. The results revealed the presence of an extracellular, thermostable and methanol-soluble metabolite that absorbed light at 212nm. These characteristics are similar to those described for cyclic antibiotic lipopeptides such as iturins.

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“…The equipment was a SHIMADZU LC-10A coupled to a detector SPD-10AV. Procedures were as those previously reported (Schreiber et al 1988;Gueldner et al 1988).…”

Section: Reverse Liquid Chromatography Of the Antibiotic Metabolitementioning

confidence: 99%

Genomic variability in Vitis vinifera L. Sangiovese assessed by microsatellite and non-radioactive AFLP test

Vignani¹,

Scali²,

Masi³

et al. 2002

Electron. J. Biotechnol.

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“…To concentrate and extract active compounds, 1.2 L of the crude filtrate were precipitated by adjusting to pH 2.5 with 3 M HCI (Gueldner et al 1988;Vanittanakom et al 1986). The precipitate was collected by centrifugation at 10 000 grfor 10 min and then extracted twice with 100%ethanol.Theextractwascentrifuged at 4000 g for 5 min and the precipitate was discarded.…”

Section: Douville Y and G J Boland 1992 A Note On The Antibiotmentioning

confidence: 99%

A note on the antibiotic properties of Bacillus subtilis against Colletotrichum trifolii

Douville

Boland

2005

phyto

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On a étudié l'influence et les mécanismes d'action in vitro et in vivoBacillus subtilissur le Colletotrichum trifolii, un champignon causant l'anthracnose de la luzerne (Medicago sativa). Lors d'essais en chambre de culture, l'application du filtrat du bouillon nutritif du B. subtilis a réduit significativement l'incidence et la gravité de la maladie de respectivement 56 à 16% et de 2,0 à 1,2. L'application de cellules lavées du B. subtilis n'a pas entraîné une diminution de l'incidence et de la gravité de la maladie. Sur les folioles de la luzerne, le filtrat a causé une réduction de la germination des conidies, la lyse des conidies et une formation réduite d'appressoriums. In vitro, il a réduit la germination des conidies, induit la lyse des conidies et la formation de tubes germinatifs enflés. Un antibiotique de la famille des iturines, l'iturine D, serait l'agent actif dans la suppression du C. trifolii par le B. subtilis.The influence and mechanisms of action of Bacillus subtilis on Colletotrichum trifolii, a causal agent of anthracnose of alfalfa (Medicago sativa), were studied in vivo and in vitro. In growth room conditions, a cell-free culture filtrate of B. subtilis significantly reduced disease incidence and severity on alfalfa seedlings from 56% to 16% and from 2.0 to 1.2, respectively. Treatment of seedlings with washed cell suspensions of B. subtilis had no influence on disease. Applications of crude filtrate on alfalfa leaflets inoculated with C. trifolii were associated with reduced germination of conidia, lysis of conidia, and reduced formation of appressoria. Under in vitro conditions, crude filtrate reduced germination of conidia, and induced lysis of conidia and the formation of inflated germ tubes on germinating conidia. An antibiotic of the iturin family, iturin D, was tentatively identified as the active compound responsible for the suppressive effect of B. subtilis on C. trifolii

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“…The two strains studied and the reference isolate of B. subtilis significantly and reproducibly reduced Pythium root rot in cauliflower seedlings. In previous studies, the application of B. subtilis to control seedling diseases has been carried out on a number of crops against a number of root and seedling pathogens (Pusey & Wilson 1984;McKeen et al 1986;Gueldner et al 1988;Klich et al 1991 in many countries (Ryder et al 1999). Paulitz & Bélanger (2001) pointed out that the most widely researched and commercialised biocontrol bacteria are species in the endospore-forming genus B. subtilis.…”

Section: Pathogenicity Testsmentioning

confidence: 99%

Biological control of root rot of cauliflower (caused byPythium ultimumvar. ultimum) using selected antagonistic rhizospheric strains ofBacillus subtilis

Abdelzaher¹

2003

New Zealand Journal of Crop and Horticultural Science

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Isolation and identification of iturins as antifungal peptides in biological control of peach brown rot with Bacillus subtilis

Cited by 108 publications

References 13 publications

Genomic variability in Vitis vinifera L. Sangiovese assessed by microsatellite and non-radioactive AFLP test

Genomic variability in Vitis vinifera L. Sangiovese assessed by microsatellite and non-radioactive AFLP test

A note on the antibiotic properties of Bacillus subtilis against Colletotrichum trifolii

Biological control of root rot of cauliflower (caused byPythium ultimumvar. ultimum) using selected antagonistic rhizospheric strains ofBacillus subtilis

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