Isolation and identification of Listeria monocytogenes utilizing DC insulator-based dielectrophoresis

Crowther, Claire V.; Hilton, Shannon Huey; Kemp, LaKeta; Hayes, Mark A.

doi:10.1016/j.aca.2019.03.019

Cited by 43 publications

(49 citation statements)

References 68 publications

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“…Each strain was tested separately in various devices of the same design. Both strains produced a pattern of collected cells appearing as distinct arcs near a 27 µm gate at appropriate applied voltages (Figures 1A, 2; Jones et al, 2014Jones et al, , 2015Crowther et al, 2019). With 1600 V applied, sv.…”

Section: Resultsmentioning

confidence: 98%

“…where ε m is the permittivity of the medium, ζ p is the EK (zeta) potential of the particle, and ζ m is the EK (zeta) potential of the medium/wall system. The value of µ EK was determined for both strains by particle tracking velocimetry (Crowther et al, 2019;Hilton and Hayes, 2019) at various applied voltages based on: where v EK is the velocity of the particle in an open channel. The capture of the particles can be observed when the flux of particles ( j ) is zero in the channel with the condition:…”

Section: Theorymentioning

confidence: 99%

“…A microchannel described in a previous work (Staton et al, 2010) and used for other cellular studies (Jones et al, 2014(Jones et al, , 2015Crowther et al, 2019) was used for the biophysical behavior study of Salmonella strains. In brief, opposing pairs of triangles were designed to constitute the sawtooth shape of the channel (Figure 1A).…”

Section: Microdevice Design Simulation and Fabricationmentioning

confidence: 99%

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Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Liu

Hayes

2020

Front. Microbiol.

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Salmonella is an important pathogen and is a worldwide threat to food safety and public health. Surveillance of serotypes and fundamental biological and biochemical studies are supported by a wide variety of established and emerging bioanalytical techniques. These include classic serotyping based on the Kauffmann-White nomenclature and the emerging whole genome sequencing strategy. Another emerging strategy is native whole cell biophysical characterization which has yet to be applied to Salmonella. However, this technique has been shown to provide high resolution differentiation of serotypes with several other paired strains of other microbes and pathogens. To demonstrate that biophysical characterization might be useful for Salmonella serotyping, the closely related strains sv. Cubana and sv. Poona were chosen for study. These two serovars were subjected to biophysical measurements on a dielectrophoresisbased microfluidic device that generated full differentiation of the unlabeled and native cells. They were differentiated by the ratio of electrophoretic (EP) to dielectrophoretic (DEP) mobilities. This differentiation factor is 2.7 ± 0.3 × 10 10 V/m 2 for sv. Cubana, versus 2.2 ± 0.3 × 10 10 V/m 2 for sv. Poona. This work shows for the first time the differentiation, concentration, and characterization of the Salmonella serotypes by exploiting their biophysical properties. It may lead to a less expensive and more decentralized new tool and method for microbiologists, complimenting and working in parallel with other characterization methods.

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Section: Resultsmentioning

confidence: 98%

Section: Theorymentioning

confidence: 99%

Section: Microdevice Design Simulation and Fabricationmentioning

confidence: 99%

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Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Liu

Hayes

2020

Front. Microbiol.

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“… to fabricate 60 µm high gold electrodes in a 150 µm high channel. For these electrodes, however, the main shortcoming is that the sample and suspending medium will be contaminated by direct contacting with the conductive microelectrodes . Recently, electrodes made of doped PDMS and doped silicon are becoming popular, which can eliminate the sample contamination problem caused by carbon and metal electrodes.…”

Section: Configurations Of Electrodes and Dep Devicesmentioning

confidence: 99%

Recent advances in dielectrophoresis‐based cell viability assessment

et al. 2020

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Recent advances in dielectrophoresis-based cell viability assessmentDielectrophoresis (DEP) is a non-destructive, accurate, and label-free cell manipulating technique and DEP applications have been found in various fields. Assessment of cell viability is one of the important applications and many investigations have been reported. In this paper, cell polarization and its modeling, some key parameters employed for living/dead cell separation, as well as electrode configurations are reviewed. Focus is given to the latest development of DEP devices employed for the assessment of cell viability. Experimentally determined factors for separating living/dead cells, such as the conductivity of suspending medium and the frequency of applied electric field, are summarized. The future directions and potential challenges in this field are also outlined. Theory of cell DEPThe fundamentals of DEP were systematically reviewed in the past [35,36]. In this section, we only focus on the theory concerning cellular DEP. Based on the spherical particle model, single-shell model and multi-shell model of biological cells are extended. Color online: See the article online to view Figs. 1-7 in color. J. Zhang et al. Electrophoresis 2020, 41, 917-932

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“…However, this approach makes use of very costly image acquisition and analysis equipment [52]. There are also insulator‐based DEP approaches that make use of field gradients created around insulating objects placed within channels [53–56]. The insulator‐based DEP approaches also use microscopes and require image analysis.…”

Section: Introductionmentioning

confidence: 99%

Parallel single‐cell optical transit dielectrophoresis cytometer

et al. 2020

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In this work, we present an optical transit DEP flow cytometer for parallel single-cell analysis. Each cell's dielectric property is inferred from velocity perturbations due to DEP actuation in a microfluidic channel. Dual LED sources facilitate velocity measurement by producing two transit shadows for each cell passing through the channel. These shadows are detected using a 256-pixel linear optical array detector. Massively parallel analysis is possible as each pixel of the detector can independently analyze the passing cells. A wide channel (∼18 mm) was employed to carry many particles simultaneously, and the system was capable of detecting the velocity of over 200 cells simultaneously. We have achieved analysis rates for 10 µm diameter polystyrene spheres response exceeding 250 per second. With appropriate calibration, this DEP cytometer can quantitatively measure the dielectric response. The dielectric response (Clausius-Mossotti factor) of viable CHO cells was measured over the frequency range of 100 kHz to 6 MHz, and the obtained response matches the previously measured values by our group. The DEP cytometer uses simple modular components to achieve high throughput label-free single-cell dielectric analysis and can begin analyzing particles within 10 s after starting to pump the sample into the channel.

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Isolation and identification of Listeria monocytogenes utilizing DC insulator-based dielectrophoresis

Cited by 43 publications

References 68 publications

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Differential Biophysical Behaviors of Closely Related Strains of Salmonella

Recent advances in dielectrophoresis‐based cell viability assessment

Parallel single‐cell optical transit dielectrophoresis cytometer

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