Isolation and identification of low-density polyethylene degrading novel bacterial strains

Nadeem, Habibullah; Alia, Khush Bakhat; Muneer, Faizan; Rasul, Ijaz; Siddique, Muhammad Hussnain; Azeem, Farrukh; Zubaır, Muhammad

doi:10.1007/s00203-021-02521-1

Cited by 39 publications

(11 citation statements)

References 34 publications

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“…Assessment of plastic polymer degradation by the PDBF01 isolate was performed by previous methods, with modi cation [57]. The isolated strain was cultured in PDA medium for 3d before transferring the mycelium from the young margin of the colony to PDB liquid medium for 7d.…”

Section: Screening Of Degradable Plasticsmentioning

confidence: 99%

Discovery of a polyvinyl alcohol-degrading strain of the ascomycete Fusarium oxysporum and optimizing of its degradation performance of PVA

Zhang,

Song,

Liu

et al. 2024

Preprint

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Massive accumulation of plastics into environment has caused enormous pressure on the ecosystem. Efficient and environmentally friendly plastics degradation technologies have evolved into a global ecological challenge. Microbial degradation, as an eco-friendly plastic treatment technology, is confronted with a problem of low efficiency in its current application. Hence, it is crucial to discovery plastic biodegradable microorganisms and find the optimal conditions for their action. The aim of our study is to isolate plastic-biodegrading fungi and explore optimum conditions for their action. A strain isolate of Fusarium oxysporum was obtained from a degraded plastic handle through screening, separation, and purification and designated PDBF01 (CGMCC No.40272). In a screening assay of plastic polymers, PDBF01 only exhibited the degradability to polyvinyl alcohol (PVA), with no activity toward polyvinyl chloride, polypropylene, or polylactic acid. PVA degradation efficiency of PDBF01 was significantly affected by inoculum concentration, temperature, and degradation time. PDBF01 produced significant degradation of PVA under 28°C and 25% inoculum concentration. Moreover, the highest degradation rate reached 51.26% after 21 days. PVA degradation rate of PDBF01 was further increased to 58.83% by the addition of electrolytes (K+, Mg2+, Fe2+, and Ca2+). Our results suggested PDBF01 can be used as a potential and efficient PVA-degrading strain in practical applications.

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Section: Screening Of Degradable Plasticsmentioning

confidence: 99%

Discovery of a polyvinyl alcohol-degrading strain of the ascomycete Fusarium oxysporum and optimizing of its degradation performance of PVA

Zhang,

Song,

Liu

et al. 2024

Preprint

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“…The by products of plastic degradation by physical and chemical methods are toxic irritants and harmful gaseous foams that not only damage the ozone layer in the upper atmosphere but also destroy the underground water resources, cause infertility of soil, prevent degradation of normal substances and are proved dangerous to human, animals and the ecosystem (Nadeem et al, 2021). Microbes are naturally performing degradation on complex organic matters, converting them into simpler organic substances and gaining nutrients during this process.…”

Section: S370mentioning

confidence: 99%

Isolation of High Density Polyethelene Degradating Bacteria from Soil

Sandip Patil,

Sandip Patil

et al. 2024

EEC

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Plastics are the most commonly used polymers for routine applications. These plastics are less biodegradable in the environment. So the plastics accumulated drastically in the environment and had bad effects on it. High-density polyethylene (HDPE) is a major cause of persistent and long-term environmental pollution. The main objective of the present study is the isolation and screening of bacterial species with the capability to degrade high-density polyethylene (HDPE). The two bacterial isolates were identified from soil collected from the dumping yard of village Atake (Tal-Karad, Dist-Satara). These two bacterial isolates were obtained on minimal agar medium incorporated with HDPE. These isolates are further tested for degradation of the plastic. The isolate 1 (Bacillus cereus) could degrade the plastic to the extent of 16% in terms of % weight loss, while the isolate 2 (Pseudomonas halobacterium) could degrade the plastic to the extent of 10% in terms of % weight loss, thus the isolate 1, which was identified as Bacillus cereus was found to be the best among the two isolates.

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“…Only module-72 appeared in the gut microbiome of antibiotic-fed larvae, in which Acinetobacter had been reported to be able to degrade PS and PU mediated by alkane-1 monooxygenase and polyurethanase, respectively, 62 which also explained why MPs can continue to depolymerize after antibiotic addition. The genera Citrobacter 63 and Serratia, 64 belonging to the specific module (Module-43) of MP groups, have been proven to be capable of degrading plastics, which explains the inhibition of MP degradation by antibiotics (Figure 5a,b).…”

Section: Mps and Antibioticsmentioning

confidence: 99%

Understanding the Ecological Robustness and Adaptability of the Gut Microbiome in Plastic-Degrading Superworms (Zophobas atratus) in Response to Microplastics and Antibiotics

Lu,

Lou,

Wang

et al. 2024

Environ. Sci. Technol.

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Recent discoveries indicate that several insect larvae are capable of ingesting and biodegrading plastics rapidly and symbiotically, but the ecological adaptability of the larval gut microbiome to microplastics (MPs) remains unclear. Here, we described the gut microbiome assemblage and MP biodegradation of superworms (Zophobas atratus larvae) fed MPs of five major petroleum-based polymers (polyethylene, polypropylene, polystyrene, polyvinyl chloride, and polyethylene terephthalate) and antibiotics. The shift of molecular weight distribution, characteristic peaks of C�O, and metabolic intermediates of residual polymers in egested frass proved depolymerization and biodegradation of all MPs tested in the larval intestines, even under antibiotic suppression. Superworms showed a wide adaptation to the digestion of the five polymer MPs. Antibiotic suppression negatively influenced the survival rate and plastic depolymerization patterns. The larval gut microbiomes differed from those fed MPs and antibiotics, indicating that antibiotic supplementation substantially shaped the gut microbiome composition. The larval gut microbiomes fed MPs had higher network complexity and stability than those fed MPs and antibiotics, suggesting that the ecological robustness of the gut microbiomes ensured the functional adaptability of larvae to different MPs. In addition, Mantel's test indicated that the gut microbiome assemblage was obviously related to the polymer type, the plastic degradability, antibiotic stress, and larval survival rate. This finding provided novel insights into the self-adaptation of the gut microbiome of superworms in response to different MPs.

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Isolation and identification of low-density polyethylene degrading novel bacterial strains

Cited by 39 publications

References 34 publications

Discovery of a polyvinyl alcohol-degrading strain of the ascomycete Fusarium oxysporum and optimizing of its degradation performance of PVA

Discovery of a polyvinyl alcohol-degrading strain of the ascomycete Fusarium oxysporum and optimizing of its degradation performance of PVA

Isolation of High Density Polyethelene Degradating Bacteria from Soil

Understanding the Ecological Robustness and Adaptability of the Gut Microbiome in Plastic-Degrading Superworms (Zophobas atratus) in Response to Microplastics and Antibiotics

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