Isolation and identification of low-density polyethylene (LDPE) biodegrading bacteria from waste landfill in Yazd

Nourollahi, Asieh; Sedighi-Khavidak, Samaneh; Mokhtari, Mehdi; Eslami, Gilda; Shiranian, Mahbubeh

doi:10.1080/00207233.2018.1551986

Cited by 20 publications

(5 citation statements)

References 21 publications

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“…Furthermore, it was established that the peak at 717 cm-1 was caused by the CH2 rock vibrational mode and that the peak at 1464 cm-1 represented CH2 bent bonds. Both of these outcomes are generally in line with the findings of Nourollahi et al (2019).…”

Section: Discussionsupporting

confidence: 86%

Screening of Fungal Isolates for Biodegradation Potentials of Low-Density Polyethylene from Selected Dumpsites

Imam,

Musa,

Idris

2023

SAJOLS

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Polyethylene is the most commonly used synthetic plastic and poorly degraded in natural environments. This study was designed to evaluate biodegradation potentials of low-density polyethylene by fugal isolates from Station Market, Kaduna State, Nigeria. Soil samples were collected at a depth of 20cm using a sterilized hand towel and taken to microbiology laboratory, Kaduna State University. Fungi were isolated and each colony was repeatedly sub-cultured on freshly prepared SDA to obtain pure isolates. Pure separated single colonies were maintained on sterile slants at 4˚C for further investigation. Low-density polyethylene (LDPE) sheets were cut into bits and immersed in xylene and boiled for 15 minutes to dissolve and crushed. The LDPE powder was added to a mineral salt medium (MSM) containing salts in 1liter of distilled water after which the fungal isolates were introduced. Scanning electron microscopy and Fourier- Transform Infrared Spectroscopy (FTIR) analysis were used to determine the changes in the polymer bond of LDPE sheet after the biodegradation. The findings shows that Aspergillus niger, Aspergillus flavus, Brown rot and White rot fungi showed 21.1%, 16.1%, 17.6%, and 18.3% reductions in the polyethylene discs after incubation respective. Therefore, the study concludes that fungal species play a significant role in the degradation of LDPE and can be used in future studies for the degradation of complex plastic materials. Finally, the study recommends that Aspergillus niger and Aspergillus falvus be considered as potential candidates to degrade LDPE.

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Section: Discussionsupporting

confidence: 86%

Screening of Fungal Isolates for Biodegradation Potentials of Low-Density Polyethylene from Selected Dumpsites

Imam,

Musa,

Idris

2023

SAJOLS

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“…Bacterial cultures Bacillus amyloliquefaciens strain BSM-1 and strain BSM-2 have shown LDPE weight loss of 11.0 % and 16.0 %, respectively, within 60 days of incubation (Das and Kumar, 2015, Harshvardhan and Jha, 2013, Bhatia et al, 2014). Pseudomonas aeruginosa strain SKN1 and strain SKN2 also showed LDPE weight loss of 10.32 % within 60 days of incubation (Nourollahi et al, 2019). In contrast to these studies, the bacterial isolate SARR1 was the most efficient strain that showed the biodegradation activity of 28.12 ± 1.09 % within 30 days of incubation only.…”

Section: Resultsmentioning

confidence: 99%

Isolation, characterization and optimization of bacterial isolate SARR1 for biodegradation of pretreated low density polyethylene

Rani

Jitender

Singh

et al. 2021

JANS

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Accumulation of low-density polyethylene (LDPE) has caused a threat to the environment because of its stable and inert nature as it cannot be degraded easily by microorganisms. Its lightweight, low cost, strength, durability, and its various other applications, have led to the wide usage of the polymer, which is exerting a negative effect on both marine and land biota. The development of an eco-friendly or a promising strategy is needed to reduce LDPE waste from both land and water. In the present study, observations have been made to isolate highly efficient LDPE degrading bacteria. The response surface methodology (RSM) was used to predict the best optimization of media for the degradation of LDPE by isolate SARR1. The isolate SARR1 was selected through primary screening by weight loss method and secondary screening using CO2 evolution test, TTC and MATH Test. The isolate SARR1 showed 6.30 ± 0.25 g/L CO2 evolution. The microbial adhesion hydrophobicity (MATH) was observed during log phase (100 to 56.89 ± 0.97 %) and stationary phase (100 to 82.92 ± 1.24 %). An isolate SARR1 converted the TTC into red coloured insoluble triphenyl formazan (TPF) after incubation of 7 days. The isolated bacteria SARR1 showed 38.3 ± 1.27 % biodegradation efficiency in the pretreated LDPE strips at 37 °C and pH 7.0 under optimized conditions within 30 days of incubation. This bioremediation and biodegradation approach is eco-friendly and safe for the environment. The results of treatment with isolate SARR1 had a potential hope to degrade LDPE at higher rate than natural degradation.

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“…Polyethylene degrading bacteria, Klebsiella pneumoniae Mk-1 was isolated from soil sample (41). In addition, Pseudomonas aeruginosa strain SKN1 and SKN2 were isolated from waste landfill which have ability to degrade low density polyethylene bacteria (42). Bacillus weihenstephanensis were isolated from garbage soil have ability to degrade LDPE and HDPE (43).…”

Section: Bacteriamentioning

confidence: 99%

Unveiling the Power Microbial Degradation by Plastic-Degrading Microorganisms

Doshi,

Chhaya

2024

IJRP

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Isolation and identification of low-density polyethylene (LDPE) biodegrading bacteria from waste landfill in Yazd

Cited by 20 publications

References 21 publications

Screening of Fungal Isolates for Biodegradation Potentials of Low-Density Polyethylene from Selected Dumpsites

Screening of Fungal Isolates for Biodegradation Potentials of Low-Density Polyethylene from Selected Dumpsites

Isolation, characterization and optimization of bacterial isolate SARR1 for biodegradation of pretreated low density polyethylene

Unveiling the Power Microbial Degradation by Plastic-Degrading Microorganisms

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