Isolation and identification of low density polythene-degrading bacteria from soil of North West of Algeria

Abstract

Plas c bags (Low Density Polyethylene (LDPE) belong to the polymers, which plays a very important role in our daily lives by their diversi ed applica on. However, the accumula on of the plas c bags in the environment cons - tutes a serious problem and a real source for visual nuisance, pollu on of soil and marine environments. Furthermore, their biodegradation was the safest method of breakdown that possibly leaves behind less toxic residues and showed poten al of bio-geo chemical cycling of the substrate. The aim of the present work was the characterization of the isolated bacterial strains from a municipal land ll area of Tlemcen, North West Algeria, which were implicated by the biodegrada on ability of the Low Density Polyethylene. The degradation of the Low Density Polyethylene was inves gated by studying the bacterial growth of the isolated, inoculated on a solid culture medium, which was composed of LDPE as the sole carbon source with and with- out a nitrogen source and the selec on was based by the determination of the produced diameter of hydrolysis clear zone on the surface. Furthermore, the isolated, selected degrading Low Density Polyethylene bacterial ML002 has been iden ed by the study of their morphological, biochemical charac- teris cs and the ampli ca on of the fragment, coding the region of ARN 16S. The use of the API system indicated their belonging to the genus Bacillus Cereus, which has reduced the weight of LDPE by 0.26, 1.28, 1.53% a er 30, 90, 120 days respec vely. Furthermore, the amplified of the fragment, coding the region of ARN 16S by the isolated, selected bacterial ML002 indicated a similarity of 99.394% with Bacillus wiedmannii and Bacillus proteolyticus and 99.293% homology with Bacillus toyonensis, Bacillus cereus and Bacillus thuringiensis.

Exploring biodegradative efficiency: a systematic review on the main microplastic-degrading bacteria

“…Thus, it can be observed that the respective studies addressed have shown a more significant effect on the degradation of modified plastics such as PS, PE, and PLA, which can be explained by these Satlewal et al, 2008;Sudhakar et al, 2008;Auta et al, 2017Auta et al, , 2018Muhonja et al, 2018;Zerhouni et al, 2018;Maroof et Rajandas et al, 2012;Shimpi et al, 2012;Yoon et al, 2012;Tribedi and Sil, 2013;Taghavi et al, 2021;Tamnou et plastic materials presenting better biodegradability (Chandra and Singh, 2020;Yuan et al, 2020). Initially, the biodegradation of MPs by bacteria occurs from the degradation of larger polymer structures to smaller particles, consequently, degradation into oligomers, dimers, and monomers, finally leading to mineralization through microbial biomass.…”

Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential

“…16S rRNA sequence analysis (1398 bp) indicated 100% similarity to the Bacillus murallis. This strain was identified as an LDPEdegrading bacteria and PE waste-colonizing bacteria in the literature [57,58].…”