Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control

Abstract

Background and Objectives: Although the study on the bacteria residing in the mid-gut, salivary gland, and reproductive organs of insect vectors have drawn appeal to the host-pathogen interactions, we know comparatively less about microbiota that naturally exist in different mosquito organs within Iran.
 Materials and Methods: In the current investigation, PCR assay by using 16S rRNA gene amplification and DNA sequenc- ing, in addition to the traditional culture-based approach utilized for the detection of cultivable bacterial assemblages in mid-gut and reproductive tracts of Culex quinquefasciatus.
 Results: The identified bacteria isolated from different tissues of 45 individuals were consisted of Achromobacter, Aero- monas, Arthrobacter, Asaia, Enterobacter, Gluconobacter, Klebsiella, Lysinibacillus, Micrococcus, Psuedomonas and Serra- tia. The results showed that Proteobacteria was the most prevalent phylum in both genders' mid-gut and reproductive tracts, and Asaia was the most common bacteria that originated in adult females and males’ tissues.
 Conclusion: These outcomes recommend that the discovered microbiome may span through Cx. quinquefasciatus popula- tions. This data can be utilized to interfere with the transmission of pathogens and design new strategies for the control of mosquito-borne diseases.

Bacterial community associated with Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) from an urban area in the Amazon, Brazil

“…The results of the current study showed that several bacterial genera found in Cx. quinquefasciatus population from Coari, Amazon state, Brazil, have been reported in other populations of the species (Wang et al, 2021;Nourani et al, 2023). Among the bacterial core we found, it is worthy to note that some are deemed potential candidates for paratransgenesis in culicids, such as Asaia and Pseudomonas (Chavshin et al, 2012).…”

“…Despite the significance of Cx. quinquefasciatus for public health (Ministério da Saúde, 2011; Serra et al, 2016;Reis et al, 2023), there are few studies regarding the microbiota of populations of this species of different regions worldwide (Ramos-Nino et al, 2020;Wang et al, 2021;Nourani et al, 2023), including Brazil (Gonçalves et al, 2019). The resistance of Cx.…”

“…Asaia is found in several mosquito species and can colonize different tissues (Favia et al, 2007;De Freece et al, 2014;Nourani et al, 2023). In addition, this bacterium can spread vertically and horizontally across mosquito populations (Mancini et al, 2016).…”