Isolation and Identification of Natural Endophytic Rhizobia from Rice (Oryza sativa L.) Through rDNA PCR-RFLP and Sequence Analysis

Singh, R. K.; Mishra, R. B.; Jaiswal, H. K.; Kumar, Vinod; Pandey, Sarvesh; Rao, Sasi B.; Annapurna, K.

doi:10.1007/s00284-005-0138-3

Cited by 57 publications

(24 citation statements)

References 19 publications

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“…These and other independent confirmations of worldwide, natural endophytic rhizobia-cereal associations and their benefit to the plant's growth physiology (Chaintreuil et al 2000;Dazzo et al 2000;Gutierrez-Zamora and Martinez-Romero 2001;Hilali et al 2001;Jha et al 2009;Lupway et al 2004;Mano and Morisaki 2008;Matiru and Dakora 2004;Mishra et al 2008;Peng et al 2008;Singh et al 2006;Singh et al 2009;Sun et al 2008;Tan et al 2001) have not lead to a technology that could be utilized directly by farmers, as positive plant-growth benefits obtained in lab, greenhouse and small experimental field plots still need meaningful "translational" research to prove the efficacy of biofertilizer inoculants under a variety of agricultural conditions, and successfully transfer those technologies to everyday use. The objective of this study was to conduct the translational research needed to assess the merit of applying this agricultural biotechnology in a large-scale rhizobial biofertilization program for rice under actual farming conditions, and by so doing, help the recent Emergency Rice Initiative achieve its important hunger-alleviating mission.…”

Section: Introductionmentioning

confidence: 75%

Enhancement of rice production using endophytic strains of Rhizobium leguminosarum bv. trifolii in extensive field inoculation trials within the Egypt Nile delta

Yanni

Dazzo

2010

Plant Soil

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This study assessed the ability of biofertilizer inoculants containing Rhizobium leguminosarum bv. trifolii to enhance production of rice (Oryza sativa L.) under actual agricultural conditions in the Nile delta. Large-scale field experiments evaluated 5 rice varieties inoculated with 7 endophytic rhizobial strains during 5 growing seasons, including at sites ranked as the world's highest in rice production. Inoculation with single strains or multi-strain consortia significantly increased grain yield in 19 of the 24 trials. By combining superior rhizobial inoculants with agricultural extension training, grain yield increased up to 47% in farmers' fields, with an average increase of 19.5%. Data on rice straw production, harvest index and the agronomic fertilizer N-use efficiency also indicated positive agronomic benefits of rhizobial inoculation. These results establish the merit of deploying our biofertilization strategy using selected rhizobial strains to promote rice production capacity while reducing the need for additional chemical Nfertilizer inputs to maintain agricultural sustainability and acceptable production economy. Technology transfer of this important translational research can significantly help to alleviate hunger and meet the nutritional needs of many people in developing countries.

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Section: Introductionmentioning

confidence: 75%

Enhancement of rice production using endophytic strains of Rhizobium leguminosarum bv. trifolii in extensive field inoculation trials within the Egypt Nile delta

Yanni

Dazzo

2010

Plant Soil

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“…Burkholderia cepacia showed the production of some pectolytic and cellulolytic enzymes (Singh et al 2006) that could possibly help them to get penetration in the intercellular spaces. The strains RRE-3 and RRE-5, used in the present study also produces a phytohormone IAA that plays an important role in colonization of bacteria by loosening of cell walls (Singh et al 2006). Fluorescent Burkholderia cepacia cells were observed on the surface of young root zones such as root hairs ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…There is tremendous pressure on agriculturists worldwide to increase the Oryza sativa yield to meet growing consumption to feed a world population increasing day by day. However, unlike symbiotic plant-microbe interactions commonly observed in legumes, in several cereal crop plants including Oryza sativa, endophytic associations have been observed (Yanni et al 1997(Yanni et al , 2001Biswas et al 2000a, b;Chaintreuil et al 2000;Chi et al 2005;Singh et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…This bacterial genus is also found to be associated with Oryza sativa and can be an important source of nitrogen in this important cereal crop (Singh et al 2006). In our recent study, we reported the isolation of two novel Burkholderia cepacia strains (RRE-3 and RRE-5) from naturally growing surface sterilized Oryza sativa roots (Singh et al 2006). To further extend our research work, we studied the colonization pattern of these strains in the seedlings of Oryza sativa variety NDR97.…”

Section: Introductionmentioning

confidence: 99%

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Colonization behavior of bacterium Burkholderia cepacia inside the Oryza sativa roots visualized using green fluorescent protein reporter

Sharma

Sharma²,

Singh

et al. 2007

World J Microbiol Biotechnol

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Colonization behavior of endophytic bacteriaBurkholderia cepacia strains RRE-3 and RRE-5 was studied in the seedlings of rice variety NDR97 using confocal laser scanning microscopy under controlled laboratory and greenhouse conditions. For studying colonization pattern, bacterial strains were tagged with pHRGFPGUS plasmid. The role of bacterial strains (both gfp/gus-tagged and untagged) in growth promotion was also studied. After coming into contact with the host root system the bacteria showed an irregular spreading. Dense colonization was observed on the primary and secondary roots and also on the junction of emergence of the lateral roots. Results showed that the colonization pattern of Burkholderia cepacia strains was similar to that of other endophytic bacteria isolated from non-legumes. Burkholderia cepacia got entry inside the root at the sites of emergence of lateral roots, without formation of infection threads as in the case of symbiotic rhizobacteria. Observations suggested that the endophytic bacterial strains RRE-3 and RRE-5 entered inside the rice roots in a progressive manner. Bacteria were found to line up along the intercellular spaces of adjoining epidermal cells adjacent to the lateral root junction, indicating endophytic colonization pattern of Burkholderia cepacia strains. Experiments with the rice seedlings inoculated with RRE-3 and RRE-5 strains revealed that both strains enhanced plant growth considerably when observed under laboratory and greenhouse conditions and produced significantly higher plant biomass. No considerable difference was observed between the gfp/gus-tagged and non-gfp/gus-tagged strains in the plant growth experiments both in the laboratory and greenhouse conditions.

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“…Inoculation of forage with rhizobial strains efficient in biological N fixation (BNF) can assist successive rice crops, either through plant residue decomposition or through an increase in N soil content. Furthermore, several studies have demonstrated that rhizobia and other symbiotic bacterial genera establish endophytic associations and promote rice growth (Yanni et al, 2001;Singh et al, 2006;Yanni and Dazzo, 2010).…”

Section: Introductionmentioning

confidence: 99%

Rhizobial Inoculation, Alone or Coinoculated with Azospirillum brasilense, Promotes Growth of Wetland Rice

Hahn

Sá

Osório

et al. 2016

Rev. Bras. Ciênc. Solo

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Rhizobia and associative bacteria promote growth in rice plants (Oryza sativa L.) through a series of mechanisms, but most studies on inoculation have been performed based on inoculation with these bacteria in a separate or singular manner. The objective of this study was to assess the efficiency of single/isolated inoculation and inoculation combined with symbiotic rhizobia from forage legume and with Azospirillum brasilense on promoting growth and the root colonization process in wetland rice. Two rhizobia among four isolates from a greenhouse and a laboratory experiment were selected that efficiently promoted seed germination and rice plant growth in a sterilized substrate and in soil. The two most efficient isolates (UFRGS Vp16 and UFRGS Lc348) were inoculated alone or in combination with a commercial product containing A. brasilense in two field experiments using two wetland rice cultivars over two growing seasons. In the field experiments, these isolates coinoculated with A. brasilense promoted larger increases in the agronomic variables of wetland rice compared to the control without inoculation. Confocal laser microscopy confirmed the presence of inoculated bacteria tagged with gfp (UFRGS Vp16, UFRGS Lc348, and A. brasilense) colonizing the root surface of the rice seedlings, mainly in the root hairs and lateral roots.

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Isolation and Identification of Natural Endophytic Rhizobia from Rice (Oryza sativa L.) Through rDNA PCR-RFLP and Sequence Analysis

Cited by 57 publications

References 19 publications

Enhancement of rice production using endophytic strains of Rhizobium leguminosarum bv. trifolii in extensive field inoculation trials within the Egypt Nile delta

Enhancement of rice production using endophytic strains of Rhizobium leguminosarum bv. trifolii in extensive field inoculation trials within the Egypt Nile delta

Colonization behavior of bacterium Burkholderia cepacia inside the Oryza sativa roots visualized using green fluorescent protein reporter

Rhizobial Inoculation, Alone or Coinoculated with Azospirillum brasilense, Promotes Growth of Wetland Rice

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