Isolation and Identification of Novel Microcystin-Degrading Bacteria

Manage, P.M.; Edwards, Christine; Singh, Brajesh K.; Lawton, Linda A.

doi:10.1128/aem.01928-09

Cited by 155 publications

(137 citation statements)

References 20 publications

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“…They observed a decrease in mlrA gene concentration during the degradation experiments, from 3.2 × 10 6 copies/L initially to 3.9 × 10 5 copies/L at day 7, which is similar to our observation of a decrease in mlrA gene concentrations in the degradation experiments ( Figure 7). The decrease in mlrA gene concentrations observed by Li et al [66] and in the current study might be due to the presence of bacteria with other degradation genes involved in the MCs' degradation process [33,38]. In addition, there was no statistically significant difference (p = 0.290) in mlrA gene evolution with or without the cyanobacteria enrichment, with a normal distribution for p = 0.49 and p = 0.21, respectively with and without the enrichment in M. aeruginosa.…”

Section: Biomolecular Aspect Of Mc-lr Degradation In Hlpl Watersupporting

confidence: 51%

Microcystin-LR Biodegradation by Bacillus sp.: Reaction Rates and Possible Genes Involved in the Degradation

Kansole

Lin

2016

Water

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Abstract:Harmful cyanobacteria blooms may deteriorate freshwater environments, leading to bad water quality that can adversely affect the health of humans, animals, and aquatic life. Many cyanobacteria can produce toxic metabolites, with Microcystin-LR (MC-LR) being the most commonly detected cyanotoxin in fresh water bodies. In this study, a MC-LR degrading Bacillus sp. strain was isolated from Hulupi Lake (HLPL), Taiwan and tested for its degradability of the cyanotoxin. The results showed that the degradation of Microcystin-LR by the isolated Bacillus sp. was temperature-dependent with an optimum MC-LR removal at 37 • C and a first order degradation constant rate for 0.22 day −1 . The degradation rate was also found to increase with decreasing MC-LR concentrations and increasing Bacillus sp. concentrations. Biomolecular monitoring of three types of genes (mlrA, CAAX, and GST) involved in the degradation indicated that mlrA, and CAAX genes were present in the indigenous bacteria in HLPL water samples. However, for the isolated Bacillus sp. strain, only CAAX genes were detected. The absence of the mlrA gene in the isolated Bacillus sp. strain shows that the degradation of MC-LR does not necessarily follow the pathways with mlrA, and can also follow the pathways involved with CAAX type II amino-terminal protease.

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Section: Biomolecular Aspect Of Mc-lr Degradation In Hlpl Watersupporting

confidence: 51%

Microcystin-LR Biodegradation by Bacillus sp.: Reaction Rates and Possible Genes Involved in the Degradation

Kansole

Lin

2016

Water

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“…In addition, a number of research groups had performed microorganism isolation with MCs served as sole carbon and nitrogen source (Valeria et al, 2006;Zhang et al, 2010) and they believed other substrates may be preferentially degraded, leading to depression of the target substrate (Markiewicz et al, 2011), while some other reports found that utilization of the target substrate were not prevented by additional carbon source. This was supported by Edwards et al (2008) who demonstrated the utilization of MCs by bacteria in river and subsequently enriched them by direct addition of MCs in the river water (Manage et al, 2009). In our studies, the TOC and TN concentrations of the media ranged from 1.08 to 5.82 mg L −1 , which were significantly higher than some isolation process (Zhang et al, 2010), resulted in delay but not inhibition of bacteria growth.…”

Section: Influence Of Nutrient Condition On Bacteria Isolationmentioning

confidence: 86%

Enhanced and continued degradation of microcystins using microorganisms obtained through natural media

Hong

Pan

2014

Journal of Microbiological Methods

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Microorganisms isolated through artificial media are often unsustainable in biodegrading microcystins (MCs) in natural water. Here we studied alternative approaches to isolate MCs-degrading bacteria using natural media. In comparison to two species (MS-1 and MS-2) isolated from artificial media and the failure of bacterial colonies formation using water extracts of sediment (10%, w/v), five colony species (WC-1 to WC-5) appeared using concentrated water extracts of sediment that is 10-fold enhancement of nutrient level. In the simulated biodegradation test in Lake Taihu water with continuous supply of MCs, a lag phase of 6 days was required for MS-1 and M-2 to degrade 13% and 15% of the added MC-RR and MC-LR, respectively, whereas the lag phase was only 3 days with approximately 44% and 31% removal of the added MC-RR and MC-LR by WC-1 to WC-5. During the continuous supply experiment, degradation of MCs by MS-1 and MS-2 stopped after 3 days, while degradation of MCs by WC-1 to WC-5 lasted continuously throughout the 18 day test period with 2 to 6-fold enhancement of removal rate. 16S rRNA gene sequences and phylogenetic analysis indicated the potential to amplify species of MCsdegrading bacteria when natural media were used. The results suggested that the increased adaptability of bacteria obtained through concentrated natural media was responsible for the enhanced and continued biodegradation under simulated natural water conditions.

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“…Microcystins possess stable chemical structure in water and thus different water treatment approaches including filtration, coagulation and flocculation are found inefficient in decreasing the concentration of these toxins in the fresh water reservoirs (Manage et al, 2009). Thus, the risk associated with their toxicity remains very high.…”

Section: Introductionmentioning

confidence: 99%

“…Presence of toxic cyanobacterial blooms in numerous natural freshwater bodies and possible use of such water in human consumption initiated work on biodegradation of cyanotoxins (Lam et al, 1995). Diversity of microbial species with ability of microcystin degradation and nodularin with tentative recognizance of novel degradation intermediates were reported (Manage et al, 2009). It was shown that MCYST can undergo biodegradation by certain aquatic bacteria like strains of the genus Sphingomonas (Saitou et al, 2003;Valeria et al, 2006) owing to the existance of an enzymatic degradation mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…Certain other bacteria like Paucibacter toxinivorans, Sphingosinicella microcystinivorans in Japan and Burkholderia (Lemes et al, 2008) were also reported as biodegraders of these cyanotoxins. The biodegradation mechanism is reported to involve a gene cluster mlrA, mlrB, mlrC and mlrD (Bourne et al, 2001;Saitou et al, 2003;Imanishi et al, 2005;Manage et al, 2009) via which particular variants, e.g., [D-Leu 1 ]MC-LR are recognized as biodegraded and/or biotransformed through aquatic microbes (Matthiensen et al, 2000). Burkholderia sp.…”

Section: Introductionmentioning

confidence: 99%

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Structure Prediction and Binding Site Analysis of Hepatotoxic Microcystin-LR Degrading MlrC-Like Protein from <i>Burkholderia</i> sp. using Computational Approaches

Singh¹,

Prabha²,

Keshri³

et al. 2016

American Journal of Bioinformatics

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Microcystin-LR (MCYST-LR) is a well characterized hepatotoxic heptapeptide produced by various species of cyanobacteria including Microcystis aeruginosa. Burkholderia is a genus of bacteria with cyanobacterial toxins degrading property. This study predicts the structure of microcystin degrading MlrC-like protein from Burkholderia sp. strain CCGE1002 that has microcystin degradation capability. Binding interaction of MlrC-like protein with MCYST-LR was studied. Threedimensional model of MlrC-like protein was generated using composite modeling based I-TASSER server. The model was further assessed through different computational approaches. The generated model was found comparable to experimental structures. MCYST-LR was used for docking with predicted model to investigate ligand-protein interaction. The study provides the structural insight into the binding mode of MlrC-like protein of Burkholderia sp. with MCYST-LR and could be further helpful in designing modeling inhibitors for MCYST-LR.

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Isolation and Identification of Novel Microcystin-Degrading Bacteria

Cited by 155 publications

References 20 publications

Microcystin-LR Biodegradation by Bacillus sp.: Reaction Rates and Possible Genes Involved in the Degradation

Microcystin-LR Biodegradation by Bacillus sp.: Reaction Rates and Possible Genes Involved in the Degradation

Enhanced and continued degradation of microcystins using microorganisms obtained through natural media

Structure Prediction and Binding Site Analysis of Hepatotoxic Microcystin-LR Degrading MlrC-Like Protein from <i>Burkholderia</i> sp. using Computational Approaches

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