Isolation and Identification of Pathogenic Bacteria Showing Resistance against Disinfectants

Johny, Bassam Oudh Al; Alkhuzaee, Abdulelah M.

doi:10.22207/jpam.13.4.18

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…Disrupting the number or ratio of microbes in the soil can potentially inhibit the recycling and transformation processes of elements or pollutants. Studies on biocide toxicity on soil bacteria and other microorganisms are limited and generally focused on biodegradation of these pollutants or antibacterial activity in the context of using them in the final consumer products [46][47][48] . In the present study, for the first time, the ecotoxicology potential of MIT and PCMX has been evaluated towards gram-negative P. putida, P. moorei, and S. mali and gram-positive B. subtilis bacterial strains by determining their growth after treatment with various concentrations of MIT and PCMX.…”

Section: Resultsmentioning

confidence: 99%

In vitro study of the ecotoxicological risk of methylisothiazolinone and chloroxylenol towards soil bacteria

Nowak-Lange

Niedziałkowska

Bernat

et al. 2022

Sci Rep

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Methylisothiazolinone (MIT) and chloroxylenol (PCMX) are popular disinfectants often used in personal care products (PCPs). The unregulated discharge of these micropollutants into the environment, as well as the use of sewage sludge as fertilizer and reclaimed water in agriculture, poses a serious threat to ecosystems. However, research into their ecotoxicity towards nontarget organisms is very limited. In the present study, for the first time, the ecotoxicity of biocides to Pseudomonas putida, Pseudomonas moorei, Sphingomonas mali, and Bacillus subtilis was examined. The toxicity of MIT and PCMX was evaluated using the microdilution method, and their influence on the viability of bacterial cells was investigated by the AlamarBlue® test. The ability of the tested bacteria to form biofilms was examined by a microtiter plate assay. Intracellular reactive oxygen species (ROS) production was measured with CM-H2DCFDA. The effect of MIT and PCMX on phytohormone indole-3-acetic acid (IAA) production was determined by spectrophotometry and LC‒MS/MS techniques. The permeability of bacterial cell membranes was studied using the SYTOX Green assay. Changes in the phospholipid profile were analysed using LC‒MS/MS. The minimal inhibitory concentrations (MICs) values ranged from 3.907 to 15.625 mg L−1 for MIT and 62.5 to 250 mg L−1 for PCMX, indicating that MIT was more toxic. With increasing concentrations of MIT and PCMX, the cell viability, biofilm formation ability and phytohormone synthesis were maximally inhibited. Moreover, the growth of bacterial cell membrane permeability and a significantly increased content of ROS were observed, indicating that the exposure caused serious oxidative stress and homeostasis disorders. Additionally, modifications in the phospholipid profile were observed in response to the presence of sublethal concentrations of the chemicals. These results prove that the environmental threat posed by MIT and PCMX must be carefully monitored, especially as their use in PCPs is still growing.

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Section: Resultsmentioning

confidence: 99%

In vitro study of the ecotoxicological risk of methylisothiazolinone and chloroxylenol towards soil bacteria

Nowak-Lange

Niedziałkowska

Bernat

et al. 2022

Sci Rep

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“…Но, несмотря на то, что перечень применяемых дезинфицирующих средств достаточно широк, в литературе не редко отмечаются случаи недостаточной эффективности дезинфицирующих средств в отношении различных видов микроорганизмов, что влияет на качество дезинфекционных мероприятий и способствует поддержанию инфекционной заболеваемости. В исследованиях, проведенных разными авторами, показана необходимость динамической оценки состояния чувствительности микроорганизмов к дезинфекционным средствам, т. к. способность микроорганизмов адаптироваться к воздействию неблагоприятных факторов, в том числе применяемых дезинфицирующих средств, обусловливает возможность формирования резистентных штаммов [1,2,3,4]. По мере развития адаптационной устойчивости микроорганизмов к применяемым средствам, проведение микробиологического мониторинга чувствительности различных штаммов микроорганизмов к дезинфектантам, является актуальным и необходимым для осуществления инфекционного контроля и оценки эффективности применяемых средств дезинфекции [5,6].…”

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Чувствительность Различных Видов Микроорганизмов К Дезинфектантам

Даулбаева,

Тастанбекова,

Адиетова

et al. 2024

Farmaciâ Kazahstana

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В статье представлены результаты изучения чувствительности различных видов микроорганизмов, выделенных из окружающей среды к современным, широко используемым в лечебно-профилактических учреждениях и лабораториях дезинфицирующим средствам. Метод. Определение чувствительности микроорганизмов к дезинфицирующим средствам проводили методом, рекомендованным Федеральными клиническими рекомендациями «Способ определения чувствительности бактерий к дезинфицирующим средствам при мониторинге устойчивости к антимикробным препаратам в медицинских организациях». Результаты. В ходе исследования, штаммы микроорганизмов: Escherichia сoli, Staphylococcus аureus, грибы рода Candida, грибы рода Penicillium показали чувствительность к дезинфектантам «Мультихлор», «Люир Окси» и «V-dez amino». Выявлена устойчивость некоторых штаммов Bacillus сereus к дезинфицирующему средству «Люир Окси».

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Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2021

EFS2

114

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The role of food-producing environments in the emergence and spread of antimicrobial resistance (AMR) in EU plant-based food production, terrestrial animals (poultry, cattle and pigs) and aquaculture was assessed. Among the various sources and transmission routes identified, fertilisers of faecal origin, irrigation and surface water for plant-based food and water for aquaculture were considered of major importance. For terrestrial animal production, potential sources consist of feed, humans, water, air/dust, soil, wildlife, rodents, arthropods and equipment. Among those, evidence was found for introduction with feed and humans, for the other sources, the importance could not be assessed. Several ARB of highest priority for public health, such as carbapenem or extended-spectrum cephalosporin and/or fluoroquinolone-resistant Enterobacterales (including Salmonella enterica), fluoroquinolone-resistant Campylobacter spp., methicillin-resistant Staphylococcus aureus and glycopeptide-resistant Enterococcus faecium and E. faecalis were identified. Among highest priority ARGs bla CTX-M , bla VIM , bla NDM , bla OXA-48like , bla OXA-23 , mcr, armA, vanA, cfr and optrA were reported. These highest priority bacteria and genes were identified in different sources, at primary and post-harvest level, particularly faeces/manure, soil and water. For all sectors, reducing the occurrence of faecal microbial contamination of fertilisers, water, feed and the production environment and minimising persistence/recycling of ARB within animal production facilities is a priority. Proper implementation of good hygiene practices, biosecurity and food safety management systems is very important. Potential AMR-specific interventions are in the early stages of development. Many data gaps relating to sources and relevance of transmission routes, diversity of ARB and ARGs, effectiveness of mitigation measures were identified. Representative epidemiological and attribution studies on AMR and its effective control in food production environments at EU level, linked to One Health and environmental initiatives, are urgently required.

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Isolation and Identification of Pathogenic Bacteria Showing Resistance against Disinfectants

Cited by 3 publications

References 14 publications

In vitro study of the ecotoxicological risk of methylisothiazolinone and chloroxylenol towards soil bacteria

In vitro study of the ecotoxicological risk of methylisothiazolinone and chloroxylenol towards soil bacteria

Чувствительность Различных Видов Микроорганизмов К Дезинфектантам

Role played by the environment in the emergence and spread of antimicrobial resistance (AMR) through the food chain

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