Isolation and Identification of Phenol and Monochlorophenols-Degrading Bacteria: <i>Pseudomonas</i> and <i>Aeromonas</i> Species

Abstract

The phenol and monochlorophenols-degrading bacteria were isolated from the soil samples adjacent to textile, pharmaceuticals, industries and automobile workshops. Twenty seven isolates were recovered including twelve phenol, four 2-chlorophenol (2-CP) and eleven 4-chlorophenol (4-CP) degrading organisms. Among them five isolates were chosen as potential monochlorophenol-degrader; four of them were identified as species of Pseudomonas and one as Aeromonas. The biodegradability of phenol and monochlorophenol followed the order: phenol &gt; 4-chlorophenol &gt; 2-chlorophenol. Pseudomonas sp. AP2, Pseudomonas sp. AP3, Pseudomonas sp. AP4 and Aeromonas sp. PP3 degraded 400 and 600 ppm phenol within 48 and 72 h, and Pseudomonas sp. A4-CP2 degraded 600 and 800 ppm phenol in 48 and 72 h respectively. Pseudomonas sp. A4-CP2 also degraded 200 ppm 2-chlorophenol within 96 h and 200 and 350 ppm 4-chlorophenol within 48 and 75 h respectively. Bacterial isolates grew well in liquid culture containing 600 ppm phenol, while the growth was scanty in the medium containing 400 or 800 ppm phenol, except for Pseudomonas sp. A4CP2. The isolate Pseudomonas sp. A4CP2 also grew on 2-CP and 4-CP at concentrations of 200 and 350 ppm respectively. The correlation coefficient (at 0.01 level of significance) between bacterial growth and degradation capability of the isolates AP2, AP3, AP4, A4CP2 and PP3 were -0.835**, -0.847**, -0.700**, -0.946** and -0.401 respectively.Keywords: Biodegradation; Phenol; 2-Chlorophenol; 4-Chlorophenol; Pseudomonas; AeromonasDOI: http://dx.doi.org/10.3329/bjm.v25i1.4854 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 41-44

Degradation of Phenol by Mixed Culture of Locally Isolated Pseudomonas Species

“…Patterns of growth were similar for all of the isolates. Pseudomonas FA also grew in the presence of 2-and 4-chlorophenol [8].…”

“…The remaining four locally isolated bacterial were Pseudomonas SA, TK, KA and FA. Identification of the local isolates was also described [8].…”

Removal of Phenol in Batch Culture by Pseudomonas putida AP11, AP9, AP6 and AP7 Isolated from the Aromatic Hydrocarbon Contaminated Soils

“…Comparing the cultural, morphological and biochemical characteristics of isolates with the properties listed in the Bargey,s Manual for systematic Bacteriology [13], it was found that all of the isolates belong to Pseudomonas putida. Pseudomonas putida are regarded as one of the most common species of phenol degrading bacteria isolated from contaminated sites [16][17][18][19]. The ability of this species particularly to utilize aromatic hydrocarbons has been widely documented [20][21].…”

“…The Pseudomonas sp. A4CP2, exhibited the highest growth (OD = 0.55) growing on 800 ppm phenol [19]. A number of bacteria have been evaluated for their usefulness in controlling phenol, a hazardous pollutant, which is produced in oil refineries, petrochemical plants, pharmaceutical industries etc.…”

Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp.

“…In addition, persistent organic pollutants (POPs), including polychlorinated biphenyls (PCBs), are associated with human activities and are subject to long-range atmospheric transport [11]. The direct discharge of phenol into the environment causes severe damage and threats to terrestrial and aquatic ecosystems and, even at low concentrations, it is extremely toxic to aquatic life [10,[12][13][14].…”