Isolation and identification of pollen allergens of

Jaggi, Kuldeep S.; Gangal, S. V.

doi:10.1016/0091-6749(87)90008-x

Cited by 19 publications

(7 citation statements)

References 26 publications

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“…Nilsen and Smestad Paulsen [16] have found seven components acting as allergens in mugwort pollen, three of them being resistant to heat treatment. In an other species, .Artemisia scoparia, Jaggi and Gangal [17] have described a heat stable and acidic allergenic component of !4 3 kD molecular weight. The relation with the heat stable 15 kD allergen we found in .Artemisia vulgaris pollen has to be investigated further.…”

Section: Discussionmentioning

confidence: 99%

A study of allergens in celery with cross‐sensitivity to mugwort and birch pollens

Vallier

Dechamp

Vial

et al. 1988

Clin Experimental Allergy

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Summary Sixty‐one sera with positive RAST to mugwort pollen (Artemisiae vulgaris) were submitted to RASTs for birch pollen (Betula verrucosa) and celery (Apium graveolens). In 36 cases RAST results were positive for celery. In addition, 23 sera presented specific IgE to birch pollen. The binding of specific IgE to individual allergens in celery, mugwort pollen and birch pollen was studied by the immunoblotting technique. This involved electrophoretic separation of allergenic extracts, electrotransfer of proteins onto nitrocellulose sheets and sensitive immunoenzymatic detection. Eighteen sera had specific IgE binding to two celery components of molecular weight around 15 kD. All these sera also detected a 15 kD allergen in mugwort and two allergens in birch of 14 kD and 16 kD molecular weight. The sera that did not detect the 15 kD bands in celery failed to react with both the 15 kD mugwort component and the 14 and 16 kD birch components. Specific cross‐inhibitions of the detection of these allergens on immunoblots were obtained by pre‐incubation of the sera with crude extract of the three species. These results strongly suggest that such allergens display some structural identity and that they could be at the origin of some cases of crossed hypersensitivity to celery, mugwort pollen and birch pollen.

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Section: Discussionmentioning

confidence: 99%

A study of allergens in celery with cross‐sensitivity to mugwort and birch pollens

Vallier

Dechamp

Vial

et al. 1988

Clin Experimental Allergy

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“…45-, 65-, and 70-kD pro tein components were found to be common in all the three extracts of F solani. Six protein components (15,17,19,24,25, and 64 kD) were unique in CF antigen, while three (28,42, and 68 kD) and one (40 kD) were unique in MY and SP antigens, respectively (table 2). Out of the 15 RAST-positivc patient sera collected, IgEspccific immunoblot analysis with five patient sera (4.…”

Section: Immunoblotmentioning

confidence: 99%

“…Percent IgE binding for individual se rum samples was determined employing a standard RAST procedure [28] using crude F. solani C'F antigen coupled toCNBr-activated paper discs [21)]. The RAST results were expressed in terms of PRU (Phadebas RAST units) by comparing the test results with RAST results o f reactions of reference birch pollen allergen discs with standard sera A -D containing specific IgE at concentrations of 17.5.…”

Section: Allergens Of Fusarium Solanimentioning

confidence: 99%

Fusarium solani: Immunochemical Characterization of Allergens

Verma

Gangal²

1994

Int Arch Allergy Immunol

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Allergenic components of the fungus Fusarium solani were isolated using (NH₄)₂SO₄ precipitation and ion-exchange column chromatography. The allergenicity of fractions was studied by enzyme-linked immunosorbent assay and radioallergosorbent test inhibition techniques. Proteins of culture filtrate (CF), mycelium (MY), and spore (SP) extracts of F. solani were characterized by isoelectrofocusing, sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and IgE-specific immunoblotting. CF antigen of F. solani contained more allergenic proteins than MY and SP, visible on immunoblot analysis using allergenic serum pool. A 65-kD protein component of CF was found to be a major allergen, as it was strongly visible on immunoblots of all 15 patient sera tested. Crossed immunoelectrophoresis and enzyme-linked immunosorbent assay inhibition using rabbit antibodies raised against F solani CF demonstrated shared antigenicity between CF, MY, and SP extracts. It was observed that F. solani is a significant allergen, and most of the allergens of MY and SP extracts were found in CF extract. Therefore, CF alone can be used in the preparation of a standard extract. However, few unique allergenic proteins were observed in MY as well as in SP extracts of F. solani. Hence, the use of combined CF, MY, and SP extracts of F. solani is recommended for diagnosis and immunotherapy.

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“…In case of weeds viz. Ageratum conyzoides, Artemisia scoparia and Ricinus communis pollen, glycoproteins of MWs 10-70 kD were found to be important IgE-binding components [15,26,27], Various workers have demonstrated the cross-reactivity among different pollen species [28,29], In the present study, shared allergenicity between the number of compo nents of PR and RC extracts was studied by IgE specific immunoblot. The protein components of MWs 92, 80, 66, 50.43 and 14 kD were observed in both the extracts by PRsensitive pooled patients sera indicating the shared aller gens among them.…”

Section: Putranjiva Roxburghii Pollen Allergensmentioning

confidence: 99%

Immunobiochemical Characterization of Putranjiva roxburghii Pollen Extract and Cross-Reactivity with Ricinus communis

Singh

Verma²,

Sridhara³

et al. 1997

Int Arch Allergy Immunol

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Putranjiva roxburghii (PR) pollen has been found to be an important aeroallergen for type I hypersensitivity. In the present study, the IgE binding proteins of PR pollen have been characterized and compared with pollen allergens of Ricinus communis (RC) belonging to family Euphorbiaceae. On isoelectric focusing, PR pollen extract resolved into 35 bands (pI 3–9), whereas SDS-PAGE separated it into 18 protein components (MW 14–100 kD). Pooled patient’s sera (ID +ve to PR) recognized 12 allergenic proteins in Putranjiva and five of them (MWs 92, 80, 55,43 and 30 kD) showed immunologic reactivity to most of the sera samples tested individually by immunoblot. A number of shared allergenic proteins (MWs 92, 80, 66, 50, 43 and 14 kD) were observed between PR and RC pollen extracts on immunoblot using Putranjiva allergic serum pool. Inhibition in the binding for most of PR pollen allergenic proteins was obtained with higher concentration of RC extract than PR itself, depicting the presence of cross-reacting allergens in both. Putranjiva pollen extract was fractionated by a combination of DEAE Sephadex-A 50 and Sephadex-G 200 column chromatography. Periodate deglycosylation of western blotted PR extract and Put I fraction indicated the involvement of carbohydrate moieties in the allergenic activity. Of the two fractions from Put I (la and lb), Put lb was found to be the most allergenic protein by ELISA inhibition. Dot blot analysis with individual patients sera identified it as a major allergen of PR.

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Isolation and identification of pollen allergens of

Cited by 19 publications

References 26 publications

A study of allergens in celery with cross‐sensitivity to mugwort and birch pollens

A study of allergens in celery with cross‐sensitivity to mugwort and birch pollens

<i>Fusarium solani</i>: Immunochemical Characterization of Allergens

Immunobiochemical Characterization of <i>Putranjiva roxburghii</i> Pollen Extract and Cross-Reactivity with <i>Ricinus communis</i>

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