Isolation and identification of Polyhydroxybutyrate (PHB)-producing microorganisms from various sources in North Sinai

Shokr, Ahmed; Abd-Elsalam, omnia; Hamedo, Hend; Elshafey, Naglaa

doi:10.21608/cat.2023.295260

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…These positive isolates were characterized by their dark bluish-black appearance when subjected to the Sudan black B staining technique. The study conducted by Shokr et al, 28 states that, among a total of 58 fungal isolates, five isolates (specifically, B1, B11, E1, D6, and N8) demonstrated the capability to accumulate PHB when observed through the application of Sudan Black B staining technique. Additionally, among 50 bacterial isolates examined in the same study,…”

Section: Sem Analysismentioning

confidence: 99%

Sustainable Synthesis of Biopolymer Polyhydroxybutyrate (PHB) from Agro-residue by Brevibacterium casei with Emphasis on Degradation Analysis

Rathna,

Kulandhaivel

2024

J. Pure Appl. Microbiol.

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The Polyhydroxybutyrate (PHB) polymer is a biodegradable microbial polyester that is intracellularly accruing due to the depletion of nitrogen and phosphorous resources and an increase in carbon supply. As part of this research investigation, Sudan Black B staining, fermentation, chloroform-sodium hypochlorite solvent-based extraction, and characterization of extracted PHB were used to isolate and identify organisms capable of producing PHB. Brevibacterium casei (OQ519751) was used to synthesize PHB biopolymer from agro-residues (orange peel, mangosteen peel, sugarcane bagasse, water hyacinth, and jackfruit peel). Using the Response Surface Methodology (RSM) and the Central Composite Design (CCD) has proven to be highly effective for optimizing PHB synthesis. The optimal conditions determined through RSM allowed Brevibacterium casei to produce significant amounts of PHB when compared to an unoptimized medium. The model demonstrated statistical significance, as indicated by the F-value of 19.96 with an associated p-value of <0.0001. Furthermore, with an optimized pH level of 7, temperature of 37°C, and yeast extract as the nitrogen source, the carbon source water hyacinth was found to synthesize an enhanced quantity of a PHB yield of 1.29 g/L from 2.2 g/L of dry biomass (58.63%). PHB characterization was done with the aid of FTIR (Fourier-transform infrared spectroscopy), XRD (X-ray diffraction), and TGA (Thermogravimetric analysis) analysis. The degradation study of PHB films was performed by soil burial method and morphological changes were scrutinized by SEM analysis. The results reveal that utilizing water hyacinth as a feedstock employs an enhanced production of PHB. This is the first report to synthesize maximum yield of PHB from Brevibacterium casei using water hyacinth as a substrate for production.

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Section: Sem Analysismentioning

confidence: 99%

Sustainable Synthesis of Biopolymer Polyhydroxybutyrate (PHB) from Agro-residue by Brevibacterium casei with Emphasis on Degradation Analysis

Rathna,

Kulandhaivel

2024

J. Pure Appl. Microbiol.

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“…PCR cycling conditions were as follows: one cycle of initial denaturation stage at 95 ºC for 3 min, followed by 35 amplification cycles [Denaturation at 95ºC for 30 s, annealing at 50 ºC for 30 s, extension at 72 ºC for 90 s], then a final extension step at 72 ºC for 5 min. PCR-DNA products (5µl) were visualized on ethidium bromide-stained 1% agarose gel (w/v) in Tris-acetate EDTA (TAE) buffer by a gel electrophoresis system (Shokr et al, 2023). Electrophoresis was performed at a constant 80V for 30min.…”

Section: Molecular Identification Of the Isolated Bacterial Strainsmentioning

confidence: 99%

Antibacterial potential of essential oils extracted from three medicinal plants against some foodborne bacteria

Serag,

Daadeer,

Elfayoumy

2023

Catrina: The International Journal of Environmental Sciences

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This study aims to evaluate the antibacterial activity of essential oils extracted from the green leaves of three medicinal plants, namely Cupressus macrocarpa, Schinus terebinthifolius, and Eucalyptus citriodora, against selected foodborne bacteria. Gas chromatography/mass spectrometry (GC/MS) analysis was employed to identify the chemical composition of the extracted essential oils. The two main chemical components of C. macrocarpa essential oils (EO) were terpinene-4-ol (32.37%) and citronellol (29.29%). The primary components of S. terebinthifolius EO were α-phellandrene (44.35%) and o-cymene (10.42%). Meanwhile, αphellandrene (13.5%) and sabinene (24.24%) constitute the majority of E. citriodora EO. The antibacterial activity of the essential oils was assessed against Gram-positive bacteria including Bacillus cereus, Staphylococcus saprophyticus, Lysinibacillus fusiformis, and Kocuria rhizophila, as well as the Gram-negative bacterium Serratia liquefaciens. Standard antibiotics like Penicillin, Amoxicillin, and Ampicillin were used for comparison. The results revealed varying degrees of antibacterial activity against each pathogenic isolate used. C. macrocarpa essential oil exhibited the strongest antibacterial action, with a 55.7 mm inhibition zone diameter against Bacillus cereus, which displayed resistance to the tested standard antibiotics. Meanwhile, essential oils of the choosed plants also inhibited the growth of S. liquefaciens despite its tolerance to the tested antibiotics. The minimum inhibitory concentrations (MIC) of C. macrocarpa, S. terebinthifolius, and E. citriodora essential oils ranged from 0.06 to 1.5 mg/ml, 0.68 to 2.0 mg/ml, and 0.2 to 1.77 mg/ml, respectively. These findings highlight the potential of the tested essential oils as antibacterial agents for preserving food materials in a safe, sustainable, cost-effective, and ecofriendly manner.

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Evaluation of Blended Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) Properties Containing Various 3HHx Monomers

Shin,

Kim,

et al. 2024

Polymers

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Polyhydroxyalkanoate (PHA), specifically poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HHx), PHBHHx) with physical properties governed by the 3-hydroxyhexanoate (3HHx) mole fraction, is a promising bioplastic. Although engineered strains used to produce P(3HB-co-3HHx) with various 3HHx mole contents and fermentation techniques have been studied, mass production with specific 3HHx fractions and monomers depends on the batch, supply of substrates, and strains, resulting in the time-consuming development of strains and complex culture conditions for P(3HB-co-3HHx). To overcome these limitations, we blended poly(3-hydroxybutyrate) [(P(3HB), produced from C. necator H16] and P(3HB-co-20 mol%3HHx) [from C. necator 2668/pCB81] to prepare films with various 3HHx contents. We evaluated the molecular weight and physical, thermal, and mechanical properties of these films and confirmed the influence of the 3HHx monomer content on the mechanical and thermal properties as well as degradability of the blended P(3HB-co-3HHx) films containing various 3HHx mole fractions, similar to that of original microbial-based P(3HB-co-3HHx). Moreover, the degradation rate analyzed via Microbulbifer sp. was >76% at all blending ratios within 2 days, whereas a weaker effect of the 3HHx mole fraction of the blended polymer on degradation was observed. P(3HB-co-3HHx) could be produced via simple blending using abundantly produced P(3HB) and P(3HB-co-20mol%HHx), and the resulting copolymer is applicable as a biodegradable plastic.

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Isolation and identification of Polyhydroxybutyrate (PHB)-producing microorganisms from various sources in North Sinai

Cited by 3 publications

References 15 publications

Sustainable Synthesis of Biopolymer Polyhydroxybutyrate (PHB) from Agro-residue by Brevibacterium casei with Emphasis on Degradation Analysis

Sustainable Synthesis of Biopolymer Polyhydroxybutyrate (PHB) from Agro-residue by Brevibacterium casei with Emphasis on Degradation Analysis

Antibacterial potential of essential oils extracted from three medicinal plants against some foodborne bacteria

Evaluation of Blended Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) Properties Containing Various 3HHx Monomers

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