Isolation and identification of Shigella spp from human fecal samples collected from Pantnagar, India

Abstract

Aim: To isolate and identify Shigella species from faecal samples based on cultural and biochemical tests. Material and Methods:For the isolation of Shigella spp., faecal samples from cattle, poultry and humans were collected from various locations of Pantnagar. Fecal specimens were processed according to standard protocols.Results: Out of 511 fecal samples (311 human, 100 cattle and 100 poultry faecal samples) analyzed, 8 isolates of Shigella species were confirmed on the basis of Gram stain, morphology, cultural identification and biochemical characters. All the 8 Shigella isolates were obtained from human stool samples giving a prevalence rate of 2.57%. Conclusion:Under the conditions of the current study, Shigella species were prevalent in human population although in small numbers, whereas it was not isolated from cattle and poultry samples.

Antibiogram Pattern of Shigella flexneri: Effect of Bio Field Treatment

“…In the present study, negative reactions (-) of VP and URE utilization tests were observed in control sample of S. flexneri. The findings were also reported in the literature [30].…”

Occurrence of Salmonella and Shigella in edible frogs (<i>Hoplobatrachus spp</i>) from Hanwa Frog market Zaria, Nigeria

“…Blackening of the stab line shows H 2 S. Other biochemical tests, viz; MR, VP, Indole and Citrate were also conducted by inoculating putative isolates as described by Cheesbrough (1984) and Gaurav et al (2013). Further confirmation was carried out using sugar fermentation test following the recommendation of (Cheesbrough, 1984).…”

Contamination of Currency Notes with Kanamycin ResistantShigella flexneri

“…Shigella species are common pathogenic bacteria which cause shigellosis that is characterized by acute bloody diarrhea (Sansonetti, 2001). The transmission of S. flexneri normally occur through fecal-oral route (Jennison and Verma, 2004) and its infections are limited to human only (Sansonetti, 2001;Gaurav et al, 2013). Annually, Shigella spp.…”