Isolation and identification of some bacteria causing subclinical mastitis in cows
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Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis
Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis, Veterinary World, 7(11): 991-998. AbstractAim: Bovine mastitis is the most economically important disease affecting dairy cattle worldwide from an economic, diagnostic and public-health point of view. The present study aimed to isolate and identify of bacteria causes mastitis in dairy cows and to evaluate the antibacterial activities of some selected medicinal plants extracts comparing antibiotics used in the treatment of mastitis in Egypt. Materials and Methods:A total of 203 milk samples of dairy cows were collected during the period from February to June 2013 at different Governorates in Egypt. The use clinical inspection and California mastitis test examination were provided efficient diagnostic tool for detection of clinical, subclinical mastitis and apparently normal health cattle. The collected milk samples were cultured on Nutrient, Blood agar, Mannitol salt, Edward's and MacConkey agar plates supporting the growth of various types of bacteria for their biochemical studies and isolation. The antimicrobial activity of plants extracts (Jasonia montana and Artemisia herb alba)with different solvent (ethanol, petroleum ether, chloroform and acetone)were studied in vitro against isolated bacteria from mastitis by paper desk diffusion and minimum inhibitory concentration method (MIC). Results:The prevalence of clinical, subclinical mastitis and normal healthy animals were 34.50%, 24.7% and 40.8% respectively. The major pathogens isolated from collected milk samples were Escherichia coli The MIC values for the extracts ranged from 0.01 to 1.56 mg/ml. when comparing antibacterial activity of A. herb alba plant extracted with acetone solvent on the same bacteria with zone of inhibition values ± SD, ranging from 00±00 to 5.6±0.60 mm. Both extracts from J. montana and A. herb alba plant extracts with petroleum ether, methanol and chloroform solvent were less antibacterial activities than acetone solvent extract. Conclusion:The present study spot highlight on isolation and identification of mastitis pathogens that are fundamental aspects of milk quality, udder health control programs and public health and food safety issues associated with food borne pathogens. J. montana and A. herb alba plants have antibacterial effects more than antibiotics used in the treatment of mastitis. Finally, the medicinal plant extracts can be used to discover bioactive natural product in the form of antibacterial that may be serve the development of new pharmaceutical products. But still need further research necessary to identify active compounds and research to mechanism and drug interaction.
Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis
Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis, Veterinary World, 7(11): 991-998. AbstractAim: Bovine mastitis is the most economically important disease affecting dairy cattle worldwide from an economic, diagnostic and public-health point of view. The present study aimed to isolate and identify of bacteria causes mastitis in dairy cows and to evaluate the antibacterial activities of some selected medicinal plants extracts comparing antibiotics used in the treatment of mastitis in Egypt. Materials and Methods:A total of 203 milk samples of dairy cows were collected during the period from February to June 2013 at different Governorates in Egypt. The use clinical inspection and California mastitis test examination were provided efficient diagnostic tool for detection of clinical, subclinical mastitis and apparently normal health cattle. The collected milk samples were cultured on Nutrient, Blood agar, Mannitol salt, Edward's and MacConkey agar plates supporting the growth of various types of bacteria for their biochemical studies and isolation. The antimicrobial activity of plants extracts (Jasonia montana and Artemisia herb alba)with different solvent (ethanol, petroleum ether, chloroform and acetone)were studied in vitro against isolated bacteria from mastitis by paper desk diffusion and minimum inhibitory concentration method (MIC). Results:The prevalence of clinical, subclinical mastitis and normal healthy animals were 34.50%, 24.7% and 40.8% respectively. The major pathogens isolated from collected milk samples were Escherichia coli The MIC values for the extracts ranged from 0.01 to 1.56 mg/ml. when comparing antibacterial activity of A. herb alba plant extracted with acetone solvent on the same bacteria with zone of inhibition values ± SD, ranging from 00±00 to 5.6±0.60 mm. Both extracts from J. montana and A. herb alba plant extracts with petroleum ether, methanol and chloroform solvent were less antibacterial activities than acetone solvent extract. Conclusion:The present study spot highlight on isolation and identification of mastitis pathogens that are fundamental aspects of milk quality, udder health control programs and public health and food safety issues associated with food borne pathogens. J. montana and A. herb alba plants have antibacterial effects more than antibiotics used in the treatment of mastitis. Finally, the medicinal plant extracts can be used to discover bioactive natural product in the form of antibacterial that may be serve the development of new pharmaceutical products. But still need further research necessary to identify active compounds and research to mechanism and drug interaction.
The present work aimed to study the pathogenicity of Archanobacterium pyogenes by using one day old chicks and chicken Embryonated eggs .The chicks were given the bacteria(4.2x10 8 CFU/ml) by orally administration and intrapertoneal injection, while the Embryonated eggs inoculated into allontoic sac at 12 days of incubation. The chicks were show dullness, decrease in food intake and nervous sings, most chicks were died after 2-3 days, and the bacteria were reisolated from internal organs, and yolk sac. The inoculation of Embryonated chicken eggs resulting in embryonicdeath, reduce hatchery percentage and deformity of embryo. The study showed that A. pyogenesisvirulence to both chicken Embryonated eggs and one day old chicks.
First genotyping confirmation of Pichia kudriavzevii in subclinically mastitic cows in Iraq
Fungal pathogens exist obviously in environment; therefore, animals may act as a source of infection to human. Pichia kudriavzevii is ubiquitous fungus of lastly great attention due to its potential use in biotechnology and processing of food, and controversial safety. This study aims to determining the prevalence rate of subclinical mastitis in lactating cows, and demonstration the presence of Pichia kudriavzevii in milk of positively mastitic cows using the molecular phylogeny. Totally, 400 adult lactating cows were subjected for collection an approximately 50 ml of fresh milk that tested initially with the California Mastitis Test (CMT); and then, positive samples have tested molecularly using conventional polymerase chain reaction (PCR). Some molecularly positive samples were analyzed phylogenetically for confirming of local isolates in the National Centre for Biotechnology Information (NCBI). Overall, 54.25% cases were positively reacted by CMT. According to score of positivity, 83.87%, 11.98% and 4.15% were showed weak, distinct, and strong positive infections, respectively. Targeting the ITS region, 28.11% of samples were reacted positively to P. kudriavzevii at 278 bp. Phylogenetic analysis of eight local P. kudriavzevii isolates showed the nucleotide alignment similarity and substitutions. Phylogenetic tree analysis revealed that the local P. kudriavzevii isolates were showed a genetic identity to the NCI-BLAST P. kudriavzevii Mexico isolates (KY646192.1) at total genetic changes ranged 0.0035-0.005%. In conclusion, this represents first molecular phylogenic study in Iraq implicates the presence of P. kudriavzevii in subclinical mastitic cows. Nationwide surveys are useful in monitoring udder health, studying the impact of structural changes, and estimating the factor(s) contribute in incidence of disease and the role of different fungi in it.
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