Isolation and Identification of Some Bacteria from Sinusitis Cases

Abstract

Microorganisms play an important role in causing diseases, sinusitis is one of these diseases which cause a health problems to many people. This study therefore was oriented to nominate the species of microorganisms isolated from Acute, Subacute and Chronic sinusitis. The causative organisms were identified by biochemical and physiological tests, and their susceptibility to selective antibiotics were tested, the Colony Forming Unit (CFU/cm 3) and the number of pus cells were also determined. The following bacterial species were isolated from sinusitis patients: Klebsiella pneumoniae (24%), Streptococcus viridans (9%), Staphylococcus aureus (8%), Bacillus spp. (6.7%), Staphylococcus epidermidis (6.1%), Streptococcus pneumoniae (4.9%), Proteus vulgaris (4%), Escherichia coli (3%), Moraxella catarrhalis (2%), Streptomyces somaliensis (1.2%), Corynebacterium diphtheriae (0.6%), Coryn. Pseudodiphtheriticum (0.6%), Coryn. Haemolyticum (0.6%), Coryn. Pyogenes (0.6%), Nocardia asteroids (0.6%), Listeria monocytogenes (0.6%), Candida albicans (0.6%). On the other hand Haemophilus influenzae could not be isolated. Chronic sinusitis dominated in male cases (11-15) years old and in female (16-20) years old. The antibiotic biogram showed that Ciprofloxacin has an activity against all bacterial isolates which were resistant to Colxacillin, Penicillin G and Ampicillin and produced β-lactamase.