Isolation and Identification of Streptomyces spp. from Desert and Savanna Soils in Sudan

Hamid, Mohamed E.; Mahgoub, Adil; Babiker, Abdulrhman J O; Babiker, H. A. E.; Holie, Mohammed Ahmed Ibrahim; Elhassan, Mogahid M.; Joseph, Martin R.P.

doi:10.3390/ijerph17238749

Cited by 12 publications

(9 citation statements)

References 31 publications

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“…Members of this group are known to have high mol% G+C because of their triple hydrogen bond of the chromosomal DNA content (Hamid et al, 2020). Streptomyces sp, Microbacterium oxydans and Aeromicrobium identified in this study having a high mol% G+C content could make them to adapt to the unfavorable environment, with low mutation rate and tolerant to antagonism factors (Hamid et al, 2020). Streptomyces spp is known to produce 80% of the antibiotic compounds according to Hamid et al (2020) which are the most important secondary metabolites of the bacterial isolate.…”

Section: Discussionmentioning

confidence: 84%

“…Streptomyces sp, Microbacterium oxydans and Aeromicrobium identified in this study having a high mol% G+C content could make them to adapt to the unfavorable environment, with low mutation rate and tolerant to antagonism factors (Hamid et al, 2020). Streptomyces spp is known to produce 80% of the antibiotic compounds according to Hamid et al (2020) which are the most important secondary metabolites of the bacterial isolate. M. oxydans and most species in this genus inhabit diverse environments and are associated with the aquatic plants as symbionts according to (Mishra et al, 2017) M. oxydans are also used in a commercial application such as food colorants, dietary supplements, cosmetics and pharmaceuticals purposes (Meddeb-Mouelhi et al, 2016).…”

Section: Discussionmentioning

confidence: 84%

“…Actinobacteria are known to produce extracellular enzymes and secondary metabolite products. Members of this group are known to have high mol% G+C because of their triple hydrogen bond of the chromosomal DNA content (Hamid et al, 2020). Streptomyces sp, Microbacterium oxydans and Aeromicrobium identified in this study having a high mol% G+C content could make them to adapt to the unfavorable environment, with low mutation rate and tolerant to antagonism factors (Hamid et al, 2020).…”

Section: Discussionmentioning

confidence: 85%

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Isolation and Characterization of Bacteria from Lakes Olbolosat and Oloiden, Kenya

Kiama¹,

Njire²,

Kambura³

et al. 2021

Afr. J. Microbiol. Res.

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There is still unexplored reservoir of microorganisms from sediments and water within Lakes Olbolosat and Oloiden using culture dependent technique. The current study compares bacterial diversity within Lake Olbolosat a freshwater lake and Lake Oloiden a saline alkaline lake. Out of 60 isolates obtained from sediments and water samples, 35 were from Lake Olbolosat and 25 from Lake Oloiden. Microbial count ranged between 0-1.75× 10 5 cfu/ml from both lakes. There was a significant difference between bacterial density and sampling points (p<0.001, F=6.667), 58 were Gram-positive and 2 Gram-negative. Fifty-five isolates that were rod-shaped, 3 were cocci and 2 filamentous. There was excellent growth of isolates at an optimum growth pH range of 6-10, a temperature range of 25-30°C and a salinity range of 0-5%. There was significant difference at p<0.001 for bacterial growth within physiological parameters. The isolates utilized skimmed milk, starch, olive oil, cellulose powder and xylan, hence the production of extracellular enzymes. There was antimicrobial activity against Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Candida albicans by bacterial isolates. BLAST analysis of partial sequences showed there were 4 different phyla. Firmicutes scored 77% closely affiliated with 20 strains, Actinobacteria scored 15% closely affiliated with 4 strains, Proteobacteria and Bacteroidetes scored 4% affiliated with 1 strain each. Novel bacteria from this study could provide insights into their diversity and biotechnological applications.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 85%

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Isolation and Characterization of Bacteria from Lakes Olbolosat and Oloiden, Kenya

Kiama¹,

Njire²,

Kambura³

et al. 2021

Afr. J. Microbiol. Res.

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“…The PCR reaction system (20 μL) included 2 μL DNA template, 10 μL Taq PCR SuperMix, 6 μL ddH 2 O, and 1 μL each of the 27F and 1492R primers. The PCR reaction program was as follows: pre-denaturation at 94°C for 5 min, denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 1 min for a total of 34 cycles, and a final extension at 72°C for 1 min ( Hamid et al, 2020 ). The PCR amplification product was checked using 1% agarose gel electrophoresis, and the remaining product was sent to Sangon Biotech (Shanghai, China) for sequencing.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Streptomyces melanosporofaciens strain X216 at controlling clubroot disease on oilseed rape

Ding,

Zhou,

Liang

et al. 2023

Front. Microbiol.

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Oilseed rape (Brassica napus L.) is highly susceptible to infection from the soilborne pathogen Plasmodiophora brassicae Woronin that causes clubroot disease and deleteriously affects production throughout the world. In this study, biological control resources were explored by isolating 237 strains of bacteria from fields of oilseed rape using the gradient dilution coating method. A strain with strong antagonistic ability was screened using a plate confrontation test and designated X216. It was identified as Streptomyces melanosporofaciens owing to its morphological characteristics and 16S rRNA gene sequence. This study also examined the lethality of strain X216 to the resting spores of P. brassicae, its influence on infection in root hairs, and its ability to control clubroot on oilseed rape. The corrected lethality rate on resting spores after strain X216 had been used for 14 days was 56.59% ± 1.97%, which was significantly higher than the use of 75% of the fungicides chlorothalonil WP and 20% Fluazinam SC. Significantly fewer root hairs were infected after this treatment. A pot test showed that X216 was 62.14% effective at controlling the disease, which was not significantly different from that of the fungicide 100 g L−1 cyazofamid SC diluted 1,000-fold but significantly higher than those of 75% chlorothalonil and 50% carbendazim WP. Strain X216 controlled 43.16% of the incidence of clubroot in the field, which could significantly reduce the disease index of oilseed rape clubroot. Therefore, strain X216 is promising to study for the biological control of oilseed rape clubroot.

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“…A significant number of identified sequences of the 16S rRNA gene were attributed to bacteria of several genera Mycobacterium (10.6%), Actinoplanes (8.5%) and Jatrophihabitans, Nocardioides, Kineosporia and Streptomyces (6.4% each). Representatives of these genera have been isolated from soil and plant substrates and are most common in the litter and phyllosphere [38][39][40][41][42][43][44]. Bacteria of the genus Mycobacterium are characterized by acid resistance [45].…”

Section: %mentioning

confidence: 99%

Soil Actinomycetes of Vietnam Tropical Forests

Dorchenkova

Грачева

Бабич

et al. 2022

Forests

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Actinomycetes are an important group of bioactive hydrolytic bacteria in any ecosystem. However, the actinomycete biodiversity in tropical ecosystems, particularly in Vietnam, is still underexplored. The aim of this article is to analyze the abundance, taxonomic structure and ecophysiological features of actinomycete complexes of soils and litter in Vietnam’s protected areas. A total of 41 samples of soils, plant litter and suspended soils were collected from six of Vietnam’s national parks and nature reserves. The direct inoculation technique showed that the total abundance of actinomycetes varied from 2.0 × 104 to 1.0 × 108 CFU/g. According to the luminescent microscopy with acridine orange dye, the length of the actinomycete mycelium was as long as 1000 m/g in the litter of Xuan Son National Park. A total of 80 strains were isolated and tested for antagonistic activity against Bacillus subtilis, Aspergillus niger and Candida albicans. Inoculation on Getchinson’s medium showed high cellulolytic activity. The most active strains were isolated from alluvial brown soil, plant litter and suspended soil of the Pu Hoat Nature Reserve. In these samples, actinomycetes adapted to high temperatures and low pH were found to be predominant. High-throughput sequencing of the V3–V4 region of the 16S rRNA gene and bioinformatic analysis confirmed the high taxonomic diversity and high hydrolytic activity of actinomycete complexes of the Pu Hoat Nature Reserve samples.

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Isolation and Identification of Streptomyces spp. from Desert and Savanna Soils in Sudan

Cited by 12 publications

References 31 publications

Isolation and Characterization of Bacteria from Lakes Olbolosat and Oloiden, Kenya

Isolation and Characterization of Bacteria from Lakes Olbolosat and Oloiden, Kenya

Efficacy of Streptomyces melanosporofaciens strain X216 at controlling clubroot disease on oilseed rape

Soil Actinomycetes of Vietnam Tropical Forests

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