Isolation and Identification of the Antimicrobial Agent Beauvericin from the Endophytic<i>Fusarium oxysporum</i>5-19 with NMR and ESI-MS/MS

Zhang, Huawei; Ruan, Chuanfen; X, Bai; Zhang, Miao; Zhu, Shuangshuang; Jiang, Yingying

doi:10.1155/2016/1084670

Cited by 14 publications

(25 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As described by Xu et al ( 2010 ), the median effective inhibitory concentration values of BEA against 6 test bacteria ( B. subtilis, S. haemolyticus, P. lachrymans, A. tumefaciens, E. coli , and X. vesicatoria ) were between 18.4 and 70.7 μg/mL. In another study (Zhang et al, 2016 ), BEA showed an inhibitory effect on three human pathogenic microbes, C. albicans, E. coli , and S. aureus . In particular, BEA exhibited the strongest antimicrobial activity against S. aureus with MIC values of 3.91 μM.…”

Section: Bioactivitymentioning

confidence: 79%

A Review on the Synthesis and Bioactivity Aspects of Beauvericin, a Fusarium Mycotoxin

Patočka

Nepovimová

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

Beauvericin (BEA) is an emerging Fusarium mycotoxin that contaminates food and feeds globally. BEA biosynthesis is rapidly catalyzed by BEA synthetase through a nonribosomal, thiol-templated mechanism. This mycotoxin has cytotoxicity and is capable of increasing oxidative stress to induce cell apoptosis. Recently, large evidence further shows that this mycotoxin has a variety of biological activities and is being considered a potential candidate for medicinal and pesticide research. It is noteworthy that BEA is a potential anticancer agent since it can increase the intracellular Ca2+ levels and induce the cancer cell death through oxidative stress and apoptosis. BEA has exhibited effective antibacterial activities against both pathogenic Gram-positive and Gram-negative bacteria. Importantly, BEA exhibits an effective capacity to inhibit the human immunodeficiency virus type-1 integrase. Moreover, BEA can simultaneously target drug resistance and morphogenesis which provides a promising strategy to combat life-threatening fungal infections. Thus, in this review, the synthesis and the biological activities of BEA, as well as, the underlying mechanisms, are fully analyzed. The risk assessment of BEA in food and feed are also discussed. We hope this review will help to further understand the biological activities of BEA and cast some new light on drug discovery.

show abstract

Section: Bioactivitymentioning

confidence: 79%

A Review on the Synthesis and Bioactivity Aspects of Beauvericin, a Fusarium Mycotoxin

Patočka

Nepovimová

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Bioassy-guided fractionation of the fermentation crude of strain R1 lead to isolation of two compounds 1 and 2 using HPLC method. By a combination of their 1D NMR and ESI-MS data as well as comparison with literature, compounds 1 and 2 were respectively characterized as dibutyl phthalate (Wu et al, 2017), beauvericin (Zhang et al, 2016) (Figure 4). And the yield of compound 1 was about 1.17 mg/L while that of compound 2 was 2.5 mg/L.…”

Section: Structure Elucidation Of Secondary Metabolites From Strain R1mentioning

confidence: 92%

“…Antimicrobial activity was assessed by the microbroth dilution method in 96-well culture plates according the procedure developed by our lab (Zhang et al, 2016). All test compounds were dissolved in DMSO (100 μg•mL -1 ).…”

Section: Antimicrobial Assay Of Compounds 1 Andmentioning

confidence: 99%

“…And the yield of compound 1 was about 1.17 mg/L while that of compound 2 was 2.5 mg/L. A number of endophytic strains had been reported to produce antimicrobial substances, such as beauvericin from F. oxysporum 5-19 colonizing in E. chrysantha L. (Zhang et al, 2016), colletonoic acid from Colletotrichum sp. endophytic on Umbelliferae (Tianpanich et al, 2011;Hussain et al, 2014).…”

Section: Structure Elucidation Of Secondary Metabolites From Strain R1mentioning

confidence: 99%

“…had been reported. As part of an ongoing effort to characterize medicinal plant-derived endophytes and their functional metabolites (Zhang et al, 2012;Zhang et al, 2016), isolation of endophytic strains from R. madaio and evaluation of antimicrobial effects of their culture extracts were carried out in this study as well as chemical investigation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial assay of endophytic fungi from Rumex madaio and chemical study of strain R1

et al. 2019

Bangladesh J Pharmacol

Self Cite

View full text Add to dashboard Cite

In order to search more antimicrobial natural products for new drug discovery, bioassay and chemical investigation of endophytic strains from Rumex madaio were carried out. Six fungal strains (numbered as L1, L6, L9, L10, R1, R2) were successfully isolated and their antimicrobial effects on three human pathogens, Escherichia coli, Staphyloccocus aureus and Candida albicans, were evaluated. The results indicated that ethyl acetate extracts of fermentation broth of these strains had no potent antimicorbial activity against E. coli and C. albicans while strains L1 and R1 had stronger inhibitory effects on S. aureus. On basis of morphological characteristics and 18S rRNA sequence analysis, strains L1 and R1 were respectively classified as Colletotrichum and Fusarium geni. The chemical study of strain R1 led to isolation of two known antimicrobial agents, dibutyl phthalate (1) and beauvericin (2).

show abstract

Novel bilayer electrospun poly(caprolactone)/ silk fibroin/ strontium carbonate fibrous nanocomposite membrane for guided bone regeneration

Etemadi

Mehdikhani

Poorazizi

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

In this study, a thin layer with a thickness of about 120 μm of poly(caprolactone) (PCL) was fabricated by electrospinning method. Then, a fibrous nanocomposite composed of PCL/silk fibroin/strontium carbonate (PCL/SF/SrCO3) was electrospun on the prepared layer. Then, they were characterized. The mechanical properties, water uptake, degradation rate, wettability, porosity, and bioactivity of the electrospun membrane were scrutinized in vitro. Cytotoxicity of the samples was assessed by using osteoblast‐like cells (SAOS‐2) and L929 fibroblasts. Moreover, the cell adhesion, alkaline phosphatase (ALP) activity, and calcium deposition through alizarin red staining were conducted. Results revealed that the bilayer structure doubled the optimum mechanical properties and the addition of SrCO3 up to 15%–20% increased ALP activity, calcium deposition, and bioactivity. According to the results, the nanofibrous bilayer membrane containing 20 wt% SrCO3, 20 wt% SF, and 60 wt% PCL was chosen as the optimum sample. Therefore, this membrane could be applied in guided bone regeneration (GBR).

show abstract

Isolation and Identification of the Antimicrobial Agent Beauvericin from the EndophyticFusarium oxysporum5-19 with NMR and ESI-MS/MS

Cited by 14 publications

References 19 publications

A Review on the Synthesis and Bioactivity Aspects of Beauvericin, a Fusarium Mycotoxin

A Review on the Synthesis and Bioactivity Aspects of Beauvericin, a Fusarium Mycotoxin

Antimicrobial assay of endophytic fungi from Rumex madaio and chemical study of strain R1

Novel bilayer electrospun poly(caprolactone)/ silk fibroin/ strontium carbonate fibrous nanocomposite membrane for guided bone regeneration

Contact Info

Product

Resources

About