Isolation and identification of two strains of pathogenic bacteria and their effects on the volatile metabolites of Gracilariopsis lemaneiformis (Rhodophyta)

Sun, Xue; He, Yanli; Xu, Nianjun; Xia, Yumei; Liu, Zhiming

doi:10.1007/s10811-011-9677-0

Cited by 19 publications

(8 citation statements)

References 29 publications

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“…This compound, together with methyl benzoate and benzaldehyde, are known to elicit very strong responses in the important pollinators Manduca sexta and Sphinx perelegans (Lepidoptera: Sphingidae) [43,44]. Benzaldehyde, which was found in high levels in leaves that had been sprayed with isolate EABb 04/01-Tip, is thought to be a biomarker for pathogenic infections in algae, together with 1-octen-3-one and E-2-nonenal [45].…”

Section: Discussionmentioning

confidence: 99%

Effects of Endophytic Entomopathogenic Ascomycetes on the Life-History Traits of Aphis gossypii Glover and Its Interactions with Melon Plants

González-Mas

Sánchez‐Ortíz

Valverde-García

et al. 2019

Insects

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Entomopathogenic fungi are sprayed commercially for aphid control in greenhouses. Recently, their ability to grow endophytically within plants was discovered, offering the opportunity for systemic biological control. Endophytic colonization of host plants could also influence life-table parameters and behavior of herbivores. We investigated lethal and pre-mortality effects of Beauveria bassiana and Metarhizium brunneum on Aphis gossypii; aphids either received inoculum while feeding on recently sprayed leaves (surface inoculum and endophytically-colonized) or while feeding on unsprayed but endophytically-colonized leaves. We used choice assays to identify any preferences for endophytically-colonized or control plants. Volatile emissions from endophytically-colonized plants and control plants were also compared. Aphid mortality rates ranged between 48.2 and 56.9% on sprayed leaves, and between 37.7 and 50.0 on endophytically-colonized leaves. There was a significant effect of endophytic colonization on the rate of nymph production, but this did not result in an overall increase in the aphid population. Endophytic colonization did not influence host-plant selection even though there were qualitative and quantitative differences in the blend of volatiles released by endophytically-colonized and control plants. Although endophytic colonization did not change herbivore behavior, plants still benefit via indirect defense, resistance to plant pathogens or abiotic stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Effects of Endophytic Entomopathogenic Ascomycetes on the Life-History Traits of Aphis gossypii Glover and Its Interactions with Melon Plants

González-Mas

Sánchez‐Ortíz

Valverde-García

et al. 2019

Insects

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“…After pre-culture, G. lemaneiformis was cultured in modified f/2 media with low nitrogen concentration (5.49 μmol·L -1 ) for four days. Prior to the experiment, the nine groups of G. lemaneiformis weighting 5.0±0.05 g each was subjected to strict bacterial removing following previous report with minor modifications (34, 35). Briefly, G. lemaneiformis was immersed in 2 L modified f/2 medium for 4 h and sonicated in a SCIENTZ JY92-ⅡN Sonicator (Ningbo Scientz Biotechnology Co., Ltd. Ningbo, China) for 10 min (50W, 5 s/5 s).…”

Section: Methodsmentioning

confidence: 99%

“…The CH 4 15N 2 O with the concentration of 75 μmol·L -1 was used as the only nitrogen source in this study. The bacterial concentration required for this study was 2.5×10 8 cells/mL determined by measured value of bacterial OD 600 (35).…”

Section: Methodsmentioning

confidence: 99%

Diversity and ecological function of urease-producing bacteria in the cultivation environment ofGracilariopsis lemaneiformis

Pei

Du²,

Aslam

et al. 2022

Preprint

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Urease-producing bacteria (UPB) provide inorganic nitrogen for primary producers by hydrolysis of urea. They play an important role in marine nitrogen cycle. However, there is still incomplete understanding of UPB and their ecological functions in the cultivation environment of red macroalageGracilariopsis lemaneiformis. This study comprehensively analyzed the diversity of culturable UPB and explored their effects on urea uptake byG. lemaneiformis. Total 34 isolates belonging to four main bacterial phyla i.e. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were identified through 16S rRNA sequencing and were screened for UPB by urea agar chromogenic medium assay andureCgene cloning. Our data revealed that only 8 strains were found containing urease. These all UPB exhibited different urease activities by Berthelot reaction colorimetry assay. Furthermore, UPB with highest urease activity was selected to use as co-culture withG. lemaneiformisto explore its role in terms of promotion or inhibition of nitrogen uptake byG. lemaneiformis. The results showed that the urea consumption in culture media and the total cellular nitrogen inG. lemaneiformisfound increased significantly in the UPB-co culture group than control i.e. in the sterile group (p< 0.05). Similarity, isotopic assay revealed that δ15N contents ofG. lemaneiformiswas significant higher in the UPB-co culture than in the control where δ15N-urea was the only nitrogen source in the culture media, indicating the UPB helpedG. lemaneiformisto absorb more nitrogen from urea. Moreover, the highest content of δ15N was found inG. lemaneiformiswith epiphytic bacteria, as compared to sterilized (control) showing that epiphytic bacteria along with UPB have compound effects in helpingG. lemaneiformisabsorb more nitrogen in urea. Taken together, these results provide unique insight into the ecological role of UPB and suggest that urease from macroalgae environment-associated bacteria might be important player in the marine nitrogen cycling.

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“…5.49 µmol•L − 1 ) for four days. Prior to the experiment, strict bacterial removal of G. lemaneiformis was performed using previous reports with minor modi cations (Sun et al, 2012;Vairappan et al, 2001). Brie y, G. lemaneiformis was immersed in 2 L modi ed f/2 medium for 4 h and sonicated in a SCIENTZ JY92-N Sonicator (Ningbo Scientz Biotechnology Co., Ltd. Ningbo, China) for 10 min (50W, 5 s/5 s).…”

Section: Urease Activity Of Upbmentioning

confidence: 99%

Diversity and ecological function of urease-producing bacteria in the cultivation environment of Gracilariopsis lemaneiformis

Pei,

Aslam,

Wang

et al. 2023

Preprint

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Urease-producing bacteria (UPB) provide inorganic nitrogen for primary producers by hydrolyzing urea, and play an important role in marine nitrogen cycle. However, there is still an incomplete understanding of UPB and their ecological functions in the cultivation environment of the red macroalgae Gracilariopsis lemaneiformis. This study comprehensively analyzed the diversity of culturable UPB and explored their effects on urea uptake by G. lemaneiformis. A total of 34 isolates belonging to four main bacterial phyla i.e. (Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria) were identified through 16S rRNA sequencing and were screened for UPB by urea agar chromogenic medium assay and ureC gene cloning. Our data revealed that only 8 strains contained urease. All of these UPB exhibited different urease activities, which were determined by the Berthelot reaction colorimetry assay. Additionally, the UPB strain (G13) isolated from G. lemaneiformis with higher urease activity was selected for co-culture with G. lemaneiformis to explore its role in promoting or inhibiting nitrogen uptake by macroalgae. The results showed a significant increase in urea consumption in the culture medium and the total cellular nitrogen in G. lemaneiformis in the UPB-co culture group compared to the sterile group (p < 0.05). This suggests that the selected UPB strain positively influences nitrogen uptake by G. lemaneiformis. Similarly, isotopic assays revealed that the δ15N content of G. lemaneiformis was significantly higher in the UPB-co culture than in the control group, where δ15N-urea was the only nitrogen source in the culture medium. This indicates that the UPB helped G. lemaneiformis to absorb more nitrogen from urea. Moreover, the highest content of δ15N was found in G. lemaneiformis with epiphytic bacteria compared to sterilized (i.e. control), showing that epiphytic bacteria, along with UPB, have a compound effect in helping G. lemaneiformis absorb more nitrogen from urea. Taken together, these results provide unique insight into the ecological role of UPB and suggest that urease from macroalgae environment-associated bacteria might be an important player in marine nitrogen cycling.

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Isolation and identification of two strains of pathogenic bacteria and their effects on the volatile metabolites of Gracilariopsis lemaneiformis (Rhodophyta)

Cited by 19 publications

References 29 publications

Effects of Endophytic Entomopathogenic Ascomycetes on the Life-History Traits of Aphis gossypii Glover and Its Interactions with Melon Plants

Effects of Endophytic Entomopathogenic Ascomycetes on the Life-History Traits of Aphis gossypii Glover and Its Interactions with Melon Plants

Diversity and ecological function of urease-producing bacteria in the cultivation environment ofGracilariopsis lemaneiformis

Diversity and ecological function of urease-producing bacteria in the cultivation environment of Gracilariopsis lemaneiformis

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