Isolation and Identification of Yeasts from Wild Flowers in Deogyu Mountain and their Physiological Functionalities

Abstract

Several kinds of yeasts were isolated from wild flowers collected from Muju resort of Deogyu mountain in Korea and identified by comparison of nucleotide sequences of D1/D2 region of 26S rDNA using BLAST. Totally twenty-two strains of eight species were isolated and Cryptococcus magnus (9 strains) and Cryptococcus flavescenes (6 strains) were dominant species. The supernatant and cell-free extracts were prepared and their antioxidant activity, anti-gout xanthine oxidase inhibitory activity and whitening tyrosinase inhibitory activity were investigated. Anti-gout xanthine oxidase inhibitory activities of Sporobolomyces phaffii JS00583 and Rhodotorula graminis JS00581 were 40.4% and 39.1%, respectively and also was 36.1% whitening tyrosinase inhibitory activity in the supernatant of Cryptococcus magnus JS00570.

Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis

“…Forty-eight wild flowers found lakeside around Jangseong Lake in Jeollanam-do, Republic of Korea were collected in August 2014, and several wild yeasts were isolated and identified as previously described [ 11 ].…”

“…The mixture was filtered to obtain cell-free extracts and supernatants. The physiological functionalities of the cell-free extracts and supernatants were determined as previously described [ 6 11 12 ].…”

Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea

“…한편, 지금까지 효모는 주로 발효식품 등에서 분리되어 Saccharomyces cerevisiae 등의 일부만이 발효식품산업에 응 용되고 있다. 최근 필자 등은 우리나라 자연 환경의 효모 종 다양성을 확립하고 나아가 국내외 미기록이나 신종 효모를 발굴하기 위하여 야생화에서 1850균주의 효모들을 분리 동 정하였고 43균주의 미기록 효모들을 발굴하여 보고하였다 [10][11][12][13][14][15][16][17][18][19]. 또한 이들 가운데 비병원성 효모들을 선별하여 항 통풍성 xanthine oxidase 저해활성을 보고하였다 [20].…”

Isolation of Wild Yeasts from Soils of Reed Fields in Seocheon-gun County, Chungcheongnam-do, South Korea, and Characterization of Unrecorded Yeasts

“…또한, 지금까지 국내에서 분리한 야생효모들의 산업적 활용을 위한 응용연구는 매우 미흡 하여 필자 등이 야생효모로부터 항고혈압성 안지오텐신 전환효소 저해물질 등 몇 가지 주요 생리 활성 물질 생산에 관해 보고하였을 뿐이다 [3,6,7,14,15]. Table 3).…”