Isolation and Immunoaffinity Purification of Biologically Active Plant Natriuretic Peptide

Billington, Timothy; Pharmawati, Made; Gehring, Chris

doi:10.1006/bbrc.1997.6883

Cited by 50 publications

(35 citation statements)

References 11 publications

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“…Isolation of irPNP. irPNP was extracted from ivy leaves ground in liquid N 2 , followed by centrifugation and ethanol liquid phase extraction and lipid removal with diethyl ether (1:1) as detailed elsewhere [16]. Aqueous extracts were freeze-dried and resuspended in double-distilled (dd) water prior to size fractionation on a Sephadex G25-80 column.…”

Section: Methodsmentioning

confidence: 99%

Natriuretic peptide hormones promote radial water movements from the xylem of Tradescantia shoots

Suwastika

Gehring

1998

CMLS, Cell. Mol. Life Sci.

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Immunological evidence suggests that plants, movements at concentrations of 50 mM, while the preslike vertebrates, contain natriuretic peptides (NPs) and ence of 10 mM 2-hydroxyethylmercaptoethanol (ME) prevents the inhibitory effect of the mercurial. The that rat atrial NP (rANP) binds specifically to plant membranes and promotes concentration and conforma-guanylate cyclase inhibitor LY 83583 at a concentration tion-dependent stomatal opening. Stomatal opening and of 20 mM and sodium azide (NaN 3 ) at concentrations of specific increases in cGMP levels were also observed in ]1 mM both also reduce radial water movements. We response to immunoreactive plant NP (irPNP). Here we therefore conclude that the regulation of radial water report that both 1 mM rANP and irPNP (100 ng total movement out of the xylem involves modulation of cGMP levels, water channels and respiration-dependent protein/100 mL) significantly increase radial water moveprocesses. In addition, we propose that NPs have a ments out of the xylem of shoots of Tradescantia multiflora. Enhanced radial water movements are also observed critical role to play in radial water movements out of the xylem and speculate that as in vertebrates, NP effects in response to the cell permeant cGMP analogue 8-Brmight, at least in part, be mediated via the regulation of cGMP (100 nM). The water channel inhibitor mercuric chloride (HgCl 2 ) sig-nificantly inhibits radial water guanylate cyclases and water channels.Key words. Natriuretic peptides in plants; water channels; cGMP; HgCl 2 ; 2 H NMR.Maintenance of water and solute homoeostasis is a key requirement for living systems. In vascular plants, water and solutes are taken up mainly via the root system and are actively or passively transported to different plant organs and cells. The bulk of water is lost via the stomatal pores, whereas ions generally remain in the system and fulfil specific structural, metabolic or signalling roles and/or are sequestered in specific organelles. Water and solute homoeostasis are coregulated by different plant hormones that, among other tasks, regulate stomatal guard cell movements, ion channel activities, compatible solute synthesis and other drought and salt stress responses (for review, see ref. 1). In this paper we demonstrate that rat atrial natriuretic peptide (rANP) and a novel immunoreactant plant natriuretic peptide hormone (irPNP) have a role in radial water movements from the xylem of Tradescantia multiflora stems and hence in water and solute homoeostasis. In vertebrates natriuretic peptide hormones are strongly implicated in the regulation of salt and water balance. Na + reabsorption occurs predominantly via apical

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Section: Methodsmentioning

confidence: 99%

Natriuretic peptide hormones promote radial water movements from the xylem of Tradescantia shoots

Suwastika

Gehring

1998

CMLS, Cell. Mol. Life Sci.

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“…Universally distributed (Table 2), NP are present in single-cell organisms (Vesely and Giordano, 1992), plants (Vesely and Giordano, 1991;Yang et al, 1999;Billington et al, 1997;Pharmawati et al, 1998) and invertebrates (Brownlee et al, 1993;Kim et al, 1994), including mollusks (Reinecke et al, 1989;Poulos et al, 1995). Their ubiquitous expression, conserved functions and high sequence homology indicate a long evolutionary history.…”

Section: Np-npr System: Molecular and Evolutionary Strategiesmentioning

confidence: 99%

Catecholamines, cardiac natriuretic peptides and chromogranin A: evolution and physiopathology of a ‘whip-brake’ system of the endocrine heart

Tota

Cerra

Gattuso

2010

Journal of Experimental Biology

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Summary In the past 50 years, extensive evidence has shown the ability of vertebrate cardiac non-neuronal cells to synthesize and release catecholamines (CA). This formed the mindset behind the search for the intrinsic endocrine heart properties, culminating in 1981 with the discovery of the natriuretic peptides (NP). CA and NP, co-existing in the endocrine secretion granules and acting as major cardiovascular regulators in health and disease, have become of great biomedical relevance for their potent diagnostic and therapeutic use. The concept of the endocrine heart was later enriched by the identification of a growing number of cardiac hormonal substances involved in organ modulation under normal and stress-induced conditions. Recently, chromogranin A (CgA), a major constituent of the secretory granules, and its derived cardio-suppressive and antiadrenergic peptides, vasostatin-1 and catestatin, were shown as new players in this framework, functioning as cardiac counter-regulators in ‘zero steady-state error’ homeostasis, particularly under intense excitatory stimuli, e.g. CA-induced myocardial stress. Here, we present evidence for the hypothesis that is gaining support, particularly among human cardiologists. The actions of CA, NP and CgA, we argue, may be viewed as a hallmark of the cardiac capacity to organize ‘whip-brake’ connection-integration processes in spatio-temporal networks. The involvement of the nitric oxide synthase (NOS)/nitric oxide (NO) system in this configuration is discussed. The use of fish and amphibian paradigms will illustrate the ways that incipient endocrine-humoral agents have evolved as components of cardiac molecular loops and important intermediates during evolutionary transitions, or in a distinct phylogenetic lineage, or under stress challenges. This may help to grasp the old evolutionary roots of these intracardiac endocrine/paracrine networks and how they have evolved from relatively less complicated designs. The latter can also be used as an intellectual tool to disentangle the experimental complexity of the mammalian and human endocrine hearts, suggesting future investigational avenues.

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“…Very few genes have been identified in plants that encode peptides that function in signaling, but PLS adds to the growing list of putative and actual plant peptide factors (reviewed in Lindsey et al, 2002). These include CLAVATA3 (Fletcher et al, 1999), the phytosulfokines (Matsubayashi et al, 1999), ENOD40 (Sousa et al, 2001), systemin (Pearce et al, 1991(Pearce et al, , 2001, the SCR proteins (Schopfer et al, 1999), and analogs of mammalian atrial natriuretic peptides (Billington et al, 1997).…”

Section: Active Rna Versus Polypeptide?mentioning

confidence: 99%

The POLARIS Gene of Arabidopsis Encodes a Predicted Peptide Required for Correct Root Growth and Leaf Vascular Patterning

et al. 2002

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The POLARIS ( PLS ) gene of Arabidopsis was identified as a promoter trap transgenic line, showing ␤ -glucuronidase fusion gene expression predominantly in the embryonic and seedling root, with low expression in aerial parts. Cloning of the PLS locus revealed that the promoter trap T-DNA had inserted into a short open reading frame (ORF). Rapid amplification of cDNA ends PCR, RNA gel blot analysis, and RNase protection assays showed that the PLS ORF is located within a short ( ‫ف‬ 500 nucleotides) auxin-inducible transcript and encodes a predicted polypeptide of 36 amino acid residues. pls mutants exhibit a short-root phenotype and reduced vascularization of leaves. pls roots are hyperresponsive to exogenous cytokinins and show increased expression of the cytokinin-inducible gene ARR5 / IBC6 compared with the wild type. pls seedlings also are less responsive to the growth-inhibitory effects of exogenous auxin and show reduced expression of the auxin-inducible gene IAA1 compared with the wild type. The PLS peptide-encoding region of the cDNA partially complements the pls mutation and requires the PLS ORF ATG for activity, demonstrating the functionality of the peptide-encoding ORF. Ectopic expression of the PLS ORF reduces root growth inhibition by exogenous cytokinins and increases leaf vascularization. We propose that PLS is required for correct auxin-cytokinin homeostasis to modulate root growth and leaf vascular patterning.

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Isolation and Immunoaffinity Purification of Biologically Active Plant Natriuretic Peptide

Cited by 50 publications

References 11 publications

Natriuretic peptide hormones promote radial water movements from the xylem of Tradescantia shoots

Natriuretic peptide hormones promote radial water movements from the xylem of Tradescantia shoots

Catecholamines, cardiac natriuretic peptides and chromogranin A: evolution and physiopathology of a ‘whip-brake’ system of the endocrine heart

The POLARIS Gene of Arabidopsis Encodes a Predicted Peptide Required for Correct Root Growth and Leaf Vascular Patterning

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