Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen.

Abstract

Lymphocytes bear many types of surface molecules, some of which are restricted to subpopulations of lymphocytes and may be necessary for their specialized immune functions. Examples of such antigens include: the Ia antigens; cell surface immunoglobulin; the Fc receptor, complement (C) receptors, and Ly-4 all present predominantly on B lymphocytes and Thy (theta) antigen; and sheep erythrocyte receptor, and the Ly-1, 2, and 3 antigens all present on T cells. H antigens (H-2 or HL-A) and ~-microglobulin are found on all populations on lymphocytes. Because a non-H-2, B-lymphocyte-specific antigen had been serologically defined on the murine lymphoblast line L1210 (reference i and footnote 1) and because a similar human, non-HL-A antigen could be present on the human B-lymphoblast line IM-1, from which the isolation of the HL-A antigen was in progress, attention has been paid to the "contaminant" molecules of the HL-A antigen preparations/ Several cell surface antigens have been identified, one of which (p23,30) is similar to Ia antigens which have been described on mouse B lymphocytes. The purification of that antigen, its distribution on distinct subsets of human lymphocytes, and the properties of heteroantisera to p23,30 in lymphocyte functional assays are described in this and the accompanying paper (3).

Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression.

“…Immunoprecipitation studies revealed that OKI 1 reacted with a bimolecular glycoprotein membrane complex of 29,000 and 34,000 dalton subunits. Antigens of a similar biochemical nature and cell surface distribution have been defined by heteroantisera, alloantisera, and monoclonal antibodies to Ia-like molecules in man, guinea pigs, and mice (1)(2)(3)(4)(19)(20)(21)(22)(23)(24)(25)(26)(27). The reactivity of OKI 1 with B lymphocytes from multiple individuals demonstrates a lack of polymorphism in the Ia-like antigen being defined by this monoclonal antibody and suggests that it recognizes a common framework determinant similar to heteroantisera described above (4).…”

“…Human Ia-like antigens were first defined by alloantisera and subsequently by heteroantisera. In man, Ia determinants are expressed on the surface of B cells, monocytes, and a subset of Null cells (1)(2)(3)(4). In contrast, Ia antigens are not readily detectable on T cells or are, at best, expressed on an extremely small percentage of resting T lymphocytes (4)(5)(6).…”

Ia-bearing T lymphocytes in man. Their identification and role in the generation of allogeneic helper activity.

“…Later, they were detected by hetero-antisera raised against the isolated protein from cell membranes (2)(3)(4). In addition to their presence on B cells and monocytes, they have been detected on the leukemic blast in eases of acute lymphocytic leukemia, acute myelogenous leukemia, and chronic myelogenous leukemia in blastic crisis (4)(5)(6).…”

Inhibition of proliferative and plaque-forming cell responses by human bone-marrow-derived lymphocytes from peripheral blood by antisera to the p23, 30 antigen.

“…Of additional interest is the mounting evidence that this antigen complex is analogous to the murine Ia antigens. p23,30 resembles murine Ia antigens with respect to chemical structure, tissue distribution, and probable linkage to the major histocompatibility complex (1)(2)(3). Moreover, antisera to p23,30, like antisera to murine Ia determinants, inhibit both mixed lymphocyte reactions and antibody-dependent cellular cytotoxicity (1,2,4,5).…”

“…This antigen complex (p23,30) was derived from the surface of a human lymphoblastoid bone-marrow-derived (B) cell line (1). p23,30 is distinct from previously identified membrane determinants of human B cells, including surface immunoglobulin, HLA antigens, beta2-microglobulin, the Fc receptor, and the complement (C) receptor.…”