Isolation and lyophilisation of anthocyanins from fruits of blackcurrant

Mariychuk, Ruslan; Eliašová, Adriana; Porubská, Janka; Poráčová, Janka; Simko, V.

doi:10.17660/actahortic.2016.1133.51

Cited by 5 publications

(2 citation statements)

References 5 publications

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“…The percentage of methanol in the solvent was at the highest value within the range as well. On the other hand, because some authors have observed that greater extraction of anthocyanins is achieved when methanol percentages are above 50%, especially from plant matrices [ 30 , 65 , 66 ], higher methanol concentrations were also tested (50–100%).…”

Section: Resultsmentioning

confidence: 99%

A Novel Ultrasound-Assisted Extraction Method for the Analysis of Anthocyanins in Potatoes (Solanum tuberosum L.)

et al. 2021

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Purple potato is one of the least known and consumed potato varieties. It is as rich in nutrients, amino acids and starches as the rest of the potato varieties, but it also exhibits a high content of anthocyanins, which confer it with some attractive health-related properties, such as antioxidant, pain-relieving, anti-inflammatory and other promising properties regarding the treatment of certain diseases. A novel methodology based on ultrasound-assisted extraction has been optimized to achieve greater yields of anthocyanins. Optimal extraction values have been established at 70 °C using 20 mL of a 60% MeOH:H2O solution, with a pH of 2.90 and a 0.5 s−1 cycle length at 70% of the maximum amplitude for 15 min. The repeatability and intermediate precision of the extraction method have been proven by its relative standard deviation (RSD) below 5%. The method has been tested on Vitelotte, Double Fun, Highland and Violet Queen potatoes and has demonstrated its suitability for the extraction and quantification of the anthocyanins found in these potato varieties, which exhibit notable content differences. Finally, the antioxidant capacity of these potato varieties has been determined by means of 2,2-diphenyl-1-picrylhydrazyl (DDPH) radical scavenging and the values obtained were similar to those previously reported in the literature.

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Section: Resultsmentioning

confidence: 99%

A Novel Ultrasound-Assisted Extraction Method for the Analysis of Anthocyanins in Potatoes (Solanum tuberosum L.)

et al. 2021

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“…Similarly, the temperature should not be above 70 • C because the solvent would start boiling and this would affect the final recovery. Some authors have observed that percentages of methanol near 70% were more optimal for the extraction of anthocyanins, especially from plant materials [19,35,49]. Finally, higher levels of methanol in the solvent were assayed while the rest of optimal extraction conditions according to the BBD were applied, i.e., 70 • C, 20 mL of solvent per 0.5 g of sample and pH = 5.…”

Section: Optimization Of the Uae Methodsmentioning

confidence: 99%

Optimization of an Ultrasound-Assisted Extraction Method for the Analysis of Major Anthocyanin Content in Erica australis Flowers

et al. 2021

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Erica australis plants have been used in infusions and folk medicine for years for its diuretic and antiseptic properties and even for the treatment of infections. In addition, a recently published thorough study on this species has demonstrated its antioxidant, antibiotic, anti-inflammatory, anticarcinogenic and even antitumoral activities. These properties have been associated with the high content of anthocyanins in E. australis leaves and flowers. The aim of the present research is to optimize an ultrasound-assisted extraction methodology for the recovery of the anthocyanins present in E. australis flowers. For that purpose, a Box Behnken design with response surface methodology was employed, and the influence of four variables at different values was determined: namely, the composition of the extraction solvents (0–50% MeOH in water), the pH level of those solvents (3–7), the extraction temperature (10–70 °C), and the sample:solvent ratio (0.5 g:10 mL–0.5 g:20 mL). UHPLC-UV-vis has been employed to quantify the two major anthocyanins detected in the samples. The extraction optimum conditions for 0.5 g samples were: 20 mL of solvent (50% MeOH:H2O) at 5 pH, with a 15 min extraction time at 70 °C. A precision study was performed and the intra-day and inter-day relative standard deviations (RSDs) obtained were 3.31% and 3.52%, respectively. The developed methodology has been successfully applied to other Erica species to validate the suitability of the method for anthocyanin extraction.

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Green synthesis of stable nanocolloids of monodisperse silver and gold nanoparticles using natural polyphenols from fruits of Sambucus nigra L.

et al. 2020

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Isolation and lyophilisation of anthocyanins from fruits of blackcurrant

Cited by 5 publications

References 5 publications

A Novel Ultrasound-Assisted Extraction Method for the Analysis of Anthocyanins in Potatoes (Solanum tuberosum L.)

A Novel Ultrasound-Assisted Extraction Method for the Analysis of Anthocyanins in Potatoes (Solanum tuberosum L.)

Optimization of an Ultrasound-Assisted Extraction Method for the Analysis of Major Anthocyanin Content in Erica australis Flowers

Green synthesis of stable nanocolloids of monodisperse silver and gold nanoparticles using natural polyphenols from fruits of Sambucus nigra L.

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