Isolation and mapping of EVx1, a human homeobox gene homologus to<i>even-skipped</i>, localized at the 5′ end of Hox1 locus on chromosome 7

Faiella, A.; D’Esposito, Maurizio; Rambaldi, M; Acampora, Dario; Balsofiore, Silvia; Stornaiuolo, Anna; Mallamaci, Antonello; Migliaccio, Enrica; Gulisano, Massimo; Simeone, Antonio

doi:10.1093/nar/19.23.6541

Cited by 38 publications

(26 citation statements)

References 34 publications

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“…eve genes in other organisms eve homologs have been identified in many animals, ranging from coral to human {Ruiz i Altaba and Melton 1989a; Bastian and Gruss 1990;D'Esposito et al 1991;Faiella et al 1991;Dush and Martin 1992;Miles and Miller 1992;Patel et al 1992;Joly et al 1993). These genes all have a highly conserved homeo domain, therefore they are thought to function as transcriptional reg- Fig.…”

Section: Discussionmentioning

confidence: 99%

“…These homeotic genes are homologous to related genes in probably all animals, and are most often arranged on the chromosome in the order in which they act along the a/p axis (for review, see McGinnis and Kmmlauf 1992;Kenyon 1994). In mice and humans, eve genes are physically linked to the "posterior" ends of two homeotic clusters (Faiella et al 1991;D'Esposito et al 1991;Dush and Martin 1992;Bastian et al 19921, and it has been argued that this is the ancestral arrangement (Doll4 et al 1994). This finding, coupled with their expression overlapping and posterior to that of homeotic genes suggests that eve genes might be involved in a/p patterning, possibly acting in concert with the HOM-C. vab-7 is not tightly linked to the C. elegans HOM-C [although it is on the same chromosome {III)], but like vertebrate eve genes, its expression is posterior to and overlaps with that of the HOM-C genes.…”

Section: A/p Axial Patterning By Vab-7 and Interaction With The Hom-cmentioning

confidence: 99%

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Posterior patterning by the Caenorhabditis elegans even-skipped homolog vab-7.

Ahringer¹

1996

Genes Dev.

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Patterning of the posterior end in animals is not well understood. Homologs of Drosophila even-skipped (eve) have a similar posterior expression pattern in many animals, and in vertebrates they are linked physically to the "posterior" ends of homeotic clusters (HOM-C), suggesting a conserved role in posterior development. However, the function of this posterior expression is not known. Here I show that the Caenorhabditis elegans gene vab-7 encodes an eve homolog that is required for posterior development and expressed in a pattern strikingly similar to that of vertebrate eve genes. Using a four-dimensional recording system, I found that posterior body muscles and the posterior epidermis are patterned abnormally in vab-7 mutants, but commitment to muscle and epidermal fates is normal. Furthermore, vab-7 activity is required for the complete expression of the most posterior HOM-C gene egl-5 in muscle cells, supporting the idea that eve homologs may act with the HOM-C to determine posterior cell fates. The conservation of sequence and expression pattern between vab-7 and eve homologs in other animals argues that most eve genes have posterior mesodermal and ectodermal patterning functions.

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Section: Discussionmentioning

confidence: 99%

Section: A/p Axial Patterning By Vab-7 and Interaction With The Hom-cmentioning

confidence: 99%

Posterior patterning by the Caenorhabditis elegans even-skipped homolog vab-7.

Ahringer¹

1996

Genes Dev.

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“…The human HD protein EVX1 [3] is closely related to the product of the Drosophila gene even-skipped {eve) which is required for the proper development of the metameric body plan of the fruit fly [4]. In mammals, the early murine Evx 1 expression pattern is compatible with a role in specifying posterior positional information along the embryonic axis while the late Evx 1 expression pattern is compatible with a role in specifying neuronal cell fates within the differentiating neural tube [5].…”

Section: Introductionmentioning

confidence: 99%

Mapping of a potent transcriptional repression region of the human homeodomain protein EVX1

Briata¹,

Ilengo²,

DeWerken³

et al. 1997

FEBS Letters

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The human homeodomain protein EVX1 is a transcriptional repressor in transfected mammalian cells and this function depends on a region carboxyl-terminal to the homeodomain. In this study, we transiently expressed several deletions of the EVX1 C-terminal region in mammalian cells and investigated their effect on the transcription of a reporter gene directed by different promoters. We show that the repressor activity maps to a region of 51 amino acids with a high abundance of alanine and proline residues. This region is able to transfer the repressor function to either the entire HOXC6 or CREB transcription factors, or to the GAL4 DNA binding domain.

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“…In the present study, three embryonic genes are of interest: EVX1, Wnt3a and Twist. The EVX1 gene is often involved in the posterior patterning of the embryo and has been shown to be modified in certain cancers (9). Expression of EVX1 is high in prostate (13).…”

Section: Discussion / Conclusionmentioning

confidence: 99%

“…EVX1 is an embryonic factor previously implied in the specification of posterior positional information within the embryo (9) and is often involved in cancer. Wnt3a induces proliferation through the ERK and Wnt/β-catenin signaling pathways and is further involved in development and cell growth (10) …”

mentioning

confidence: 99%