Isolation and Metastatic Properties of Detachment Variants of 816 Melanoma Cells 2

Abstract

Variant clones of B16 melanoma cells have been isolated that detached from plastic tissue culture dishes either more or less readily than the parent line upon treatment with EDTA or ethyleneglycol-bis(b-amino ethyl ether)N,N'-tetraacetic acid. Cells that detached more readily were spindlier in culture than parent cells, covered less surface area per cell, and formed fewer pulmonary tumors when injected iv into C57BL/6J mice. Cells that detached less readily were flatter in culture and formed more pulmonary tumors when injected iv than the parent cells. After detachment with EDTA, the cells reattached to culture dishes at the same rate.

Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties

“…This is similar to that reported by Hart (1979) for lung colonizing variants of B16 melanoma, but is inconsistent with other models (Varani et al, 1980). It is of interest that Briles and Kornfeld (1978) selected colonizing variants of B16 melanoma cells by differential EDTA release from plastic, a procedure which resulted in lines with different clonal morphology.…”

“…Various stages of the metastatic cascade, for example local invasion or tumourcapillary endothelium adhesion are partially dependent upon intercellular and cell-substrate adhesion and motility (Weiss, 1980;Varani et al, 1980;Nicolson et al, 1986). The cell surface has an important role in these interactions and has been extensively studied for correlations with metastasis and organ colonization (Turner, 1982;Nicolson, 1984).Observation and measurement of adhesion and motility in vivo have inherent difficulties, but these characters can be investigated in vitro and some clues about their possible significance in vivo thereby gained (Briles & Kornfeld, 1978; Hart, 1979;Vollmers & Birchmeier, 1983;McCarthy et al, 1985).We have, therefore, looked at the adhesive and motile properties of tight and loose cells and also examined their cell surfaces by lectin binding to PAGE separated glycoproteins, radio-iodination of proteins and quantitation of sialic acid content. (Sidebottom & Clark, 1983).…”

“…Observation and measurement of adhesion and motility in vivo have inherent difficulties, but these characters can be investigated in vitro and some clues about their possible significance in vivo thereby gained (Briles & Kornfeld, 1978;Hart, 1979;Vollmers & Birchmeier, 1983;McCarthy et al, 1985).…”

Anti-tumor activity mediated by protein and peptide transduction of HIV viral protein R (Vpr)

“…HeLa 33 (human cervical carcinoma), SK-N-AS 34 (human neuroblastoma), TE671, 35 (human meduloblastoma), ZR75-1, 36 (human breast carcinoma), THP1, 37 (human acute monocytic leukemia), LnCap 38 (androgen-sensitive human prostate adenocarcinoma) and B16.F10, 39 (murine melanoma) tumor cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA). The HeLa, TE671 and SK-N-AS cell lines were grown in a Dulbecco's Modified Eagle's medium while the murine B16.F10 melanoma cell line was cultured in McCoy's 5A medium.…”

Dysfunction of Murine Dendritic Cells Induced by Incubation with Tumor Cells

“…Hepa 1-6 cell line was a derivative of the BW7756 mouse hepatoma that arose in a C57BL/6 mouse (ATCC CRL-1830) (13). B16 cell line was derived from C57BL/6 mouse skin melanoma (ATCC CRL-6475) (14). Hepa 1-6 and B16 cells were cultured in Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 10% FBS.…”