Isolation and Molecular Characterisation of TtDro1A and TtDro1B Genes from Triticum turgidum Subspecies durum and turgidum, Study of Their Influences on Seedling Root Angles

Loarce, Yolanda; Cabeza, Alejandra; Cañas, Rodrigo; González, Juan M.

doi:10.3390/plants11060821

Cited by 3 publications

(1 citation statement)

References 63 publications

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“…This suggests that LAZY proteins promote narrowed lateral shoot and root angles in response to gravity. Consistent with this, overexpression of some LAZY homologs led to narrower branch and root angles and enhanced gravitropism 17–19 . LAZY homologs also are involved in responses to light quantity and quality, likely functioning to integrate light and gravity signaling during the establishment of the default lateral organ angle or “set-point angle” 15,20,21 .…”

Section: Introductionsupporting

confidence: 60%

Working smarter, not harder: silencingLAZY1inPrunus domesticacauses outward, wandering branch orientations with commercial and ornamental applications.

Kohler,

Hollender,

Raines

et al. 2024

Preprint

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Controlling branch orientation is a central challenge in tree fruit production, as it impacts factors as diverse as light interception, pesticide use, fruit quality, yield, and labor costs. In an attempt to modify branch orientation, growers use many different management practices, including tying branches to wires or applying growth regulator sprays. However, these practices are often costly and ineffective. In contrast, altering the expression of genes that control branch angles and orientations would permanently optimize tree architecture with minimal management inputs. One gene implicated in branch angle control isLAZY1, which promotes upward branch growth in response to gravity. We used an antisense vector to silenceLAZY1in plum (Prunus domestica). We found that theseLAZY1-silencedlines have significantly increased branch and petiole angles. In addition, they lack apical dominance and display a wandering or weeping branch trajectory. Given these phenotypes, we assessed whether the strength or stiffness of the branches were compromised. No differences were observed in new growth. While the wood of first-yearLAZY1-silenced branches was more flexible and weaker than the control, the strength and stiffness of the branches were not decreased because branch diameter is increased. Finally, we evaluated the utility ofLAZY1-silenced trees for two planar orchard systems, training them in super slender axe and espalier.LAZY1-silenced trees had more open canopies and were easier to constrain to the trellis height. This work illustrates the power of manipulating gene expression to optimize plant architecture for horticultural applications.

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Section: Introductionsupporting

confidence: 60%

Working smarter, not harder: silencingLAZY1inPrunus domesticacauses outward, wandering branch orientations with commercial and ornamental applications.

Kohler,

Hollender,

Raines

et al. 2024

Preprint

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Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress

Zhao,

Yao,

Peng

et al. 2024

Horticultural Plant Journal

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Study of Variability in Root System Architecture of Spanish Triticum turgidum L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the TtDro1B Gene: Evolutionary Implications

et al. 2021

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We analysed nine traits of the root system of 223 genotypes of Triticum turgidum (2n = 4x = AABB) subspecies dicoccoides, dicoccum, turgidum, durum and polonicum, finding a large intra and interspecific variability in both the number and size of roots, as well as in their spatial distribution. We studied the presence of an incomplete MITE (Miniature Inverted-repeat Transposable Element) inserted in the TtDro1B gene, which is present in some genotypes of dicoccoides, dicoccum, and turgidum, but not in polonicum and the 97.9% of the durum accessions. Comparison between genotypes shows that genotypes with the MITE element have smaller and shallower roots. Since Aegilops is considered to be the donor of the wheat B genome, the presence of the same MITE element was analysed in 55 accessions of the species Aegilops speltoides, searsii, bicornis and longissima, and in no case was it detected. We propose that after the emergence of T. turgidum subsp. dicoccoides, the insertion of the MITE element probably occurred in a single plant. Subsequent domestication resulted in genotypes of dicoccum with and without the MITE element, which after selection gave rise to the subspecies turgidum, and durum and polonicum, respectively. The MITE element can be used to differentiate turgidum from the durum and polonicum with high reliability.

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Isolation and Molecular Characterisation of TtDro1A and TtDro1B Genes from Triticum turgidum Subspecies durum and turgidum, Study of Their Influences on Seedling Root Angles

Cited by 3 publications

References 63 publications

Working smarter, not harder: silencingLAZY1inPrunus domesticacauses outward, wandering branch orientations with commercial and ornamental applications.

Working smarter, not harder: silencingLAZY1inPrunus domesticacauses outward, wandering branch orientations with commercial and ornamental applications.

Splicing defect of StDRO2 intron 1 promotes potato root growth by disturbing auxin transport to adapt to drought stress

Study of Variability in Root System Architecture of Spanish Triticum turgidum L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the TtDro1B Gene: Evolutionary Implications

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