Isolation and molecular characteristics of extended spectrum beta-lactamase-producing uropathogenic Escherichia coli isolated from hospital attendees in Ebonyi State, Abakaliki

Ugbo, Emmanuel Nnabuike; Anyamene, C. O.; Moses, I B; Ariom, T O; Agumah, N. B.; Chukwunwejim, C R; Egbule, C.U.; Emioye, A.A.; Okata-Nwali, O D; Aneke, Chinwe J.; Ugadu, I O; Osu, B O

doi:10.5897/ajb2020.17251

Cited by 3 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, multiple ESBL-producing E. coli strains were identified to harbour blaCTX-M and blaTEM with a prevalence rate of 12 (40.0%). This is in line with the finding of other research in Nigeria, where ESBL-producing E. coli were discovered to harbour blaCTX-M and blaTEM in multiple instances, being prevalent at a rate of 15.3% (25).…”

Section: Discussionsupporting

confidence: 92%

Prevalence of the bla _CTX-M and bla _TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Faridah

Wibisono

et al. 2023

Journal of Veterinary Research

View full text Add to dashboard Cite

Introduction Infections of humans and animals by multidrug resistant bacteria are increasing because of the inappropriate use of antibiotics. Disease management may be more challenging if Escherichia coli produce extended-spectrum beta-lactamase (ESBL), which could cause resistance to aztreonam and third-generation cephalosporins. This study was aimed at determining the prevalence of the bla CTX-M and bla TEM genes among ESBL-producing E. coli isolated from broiler chickens in Indonesia. Material and Methods A total of 115 broiler cloacal swab samples were obtained from 22 farms and studied for the presence of E. coli. The isolates were identified using approved standard methods and were purified on eosin methylene blue agar media. The E. coli isolates were subjected to sensitivity testing using beta-lactam antibiotics, and ESBL production was confirmed by a double-disc synergy test. The presence of the bla CTX-M and bla TEM genes was identified using a PCR. Results It was found that 99/115 (86.1%) of the isolated E. coli were resistant to beta-lactam antibiotics and 34/115 (29.6%) of them were phenotypically detected to be ESBL producers. Of the 34 isolates that were confirmed ESBL producers, 32/34 (94.1%) of them harboured the bla CTX-M and 13/34 (38.2%) the bla TEM genes. The bla CTX-M and bla TEM genes were detected together in 12/34 (35.3%) isolates. Conclusion This study discovered that broiler chickens are possible reservoirs of ESBL-producing E. coli that may infect humans. Thus, a committed public health education campaign is recommended in order to mitigate the potential threat to human health.

show abstract

Section: Discussionsupporting

confidence: 92%

Prevalence of the bla _CTX-M and bla _TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Faridah

Wibisono

et al. 2023

Journal of Veterinary Research

View full text Add to dashboard Cite

show abstract

“…In addition, the resistance rate to cephalosporins were relatively high i.e., Cefotaxime, Cefoxtin Ceftazidime, Ceftriaxone was observed at 60-100% and is in support with the findings of Yan et al [56] were Carbapenem-resistant Klebsiella pneumoniae isolates demonstrated 78.2% and 75.6% resistant to ceftriaxone and ceftazidime with similar findings reporting cefoxitin 83.3%, ceftriaxone 100%, ceftazidime 95.8% [40]; ceftazidime 66.7% and ceftriaxone 92.3% [57] and more recent study documented carbapenem-resistant Klebsiella pneumoniae isolates 100% resistant to ceftriaxone and ceftazidime [58]. Also, this finding correlate with an earlier study in the same setting (Abakaliki) were resistant to cefotaxime (83.6%), ceftazidime (79.5%) and ceftriaxone (57.5%) were reported [59] while Adabara et al [60] in Minna reported resistant to cefotaxime (84.6%). This study conducted also established that most Carbapenem-resistant isolates are multidrug resistant (MDR), especially to 3rd and 4th generation cephalosporins.…”

Section: Key: N= Number Of Isolate Cpr-carbapenem Resistant Cps-carba...supporting

confidence: 85%

“…The MDR resistant trend of carbapenem-resistant isolates to trimethoprim/ sulfamethoxazole, amoxicillin/clavulanate, azetronam and tetracycline in this study has not changed from the reported pattern in earlier studies [40,59,61,62,63,64]. Additionally, the high percentages of Carbapenem-resistant isolates cross-resistance observed to various antimicrobials are of concern in clinical medicine, especially in intensive care units and other relevant unit.…”

Section: Key: N= Number Of Isolate Cpr-carbapenem Resistant Cps-carba...contrasting

confidence: 38%

Prevalence and Antibiogram Profile of Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae among Patients with Urinary Tract Infection in Abakaliki, Nigeria

Nomeh

Chukwu²,

Ogba³

et al. 2022

IJPR

View full text Add to dashboard Cite

Background and Objectives: Carbapenem antibiotic are drug of last-resort from the treatment of bacterial infection, as a result of the prevalence and rapidly evolving enzymes from Carbapenem resistant bacteria such Escherichia coli and Klebsiella pneumoniae make urinary tract infection difficult, and in some cases impossible to treat in health care settings. With limited progress of new antibacterial drugs, the best approach is monitoring the prevalence and antibiogram profile of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae among patients with UTI in Abakaliki, Nigeria. Methodology: A non-repetitive, clean catch mid-stream urine was collected from five hundred (500) diagnosed UTI inpatient and outpatient. The samples were evaluated using routine microbiological protocol for isolation and identification of Escherichia coli and Klebsiella pneumoniae. Phenotypic screening of Carbapenem-resistant strains was performed using Modified Hodge Testing. Antibiogram studies of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae was performed using the Kirby–Bauer disk diffusion method and the results were interpreted using the Clinical Laboratory Standard Institute (CLSI) zone diameter breakpoints. Multiple antibiotic resistance index (MARI) was determined for MDR strain. Result: The prevalence of Escherichia coli and Klebsiella pneumoniae isolate accounted for 148(29.6 %) consisting of 95(54.3 %) and 53(16.3 %) from in-patients and out-patients. Escherichia coli accounted overall isolation rate of 112(22.4 %) comprising of high proportion among in-patient 82(46.9 %) over out-patient 30(9.2 %). The proportion of K. pneumoniae accounted for 36(7.2 %) with 13(7.4 %) and 23(7.1 %) recorded among in-patients and out-patients. Association between presence of Escherichia coli and Klebsiella pneumoniae isolates in clinical samples was statistically significant with patient’s population with p value <0.05. Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae accounted for 37(7.4 %) comprising of 24(13.7) and 13(4.0 %) among in-patients and out-patients respectively while carbapenem-susceptible Escherichia coli and Klebsiella pneumoniae accounted for overall detection rate of 111(22.2 %) consisting of 71(40.6 %) and 40(12.3 %) among in-patients and out-patients respectively. The isolates resistance rate to cephalosporins were relatively high i.e., Cefotaxime, Cefoxtin Ceftazidime, Ceftriaxone resistance was observed at 60-100% while amoxicillin/clavulanate, azetronam, tetracycline nitrofurantoin and Ticarcillin-clavulanic acid recorded 100 % with MDR index ranged from 0.5-0.8, but were 100 % and 85.0 % sensitive to ciprofloxacin and ofloxacin. Conclusion: These results strongly hypothesize that MDR bacteria, including Carbapenem-resistant isolate, have become common residents in various hospital environments, however with substantial evidence in this study, ciprofloxacin and ofloxacin as drugs of choice could be used for treatment of UTI. Therefore, its importance that good antibiogram evaluation of other drug classes beside fluoroquinoles reported in this study need to be establishes as baseline for empirical diagnosis, epidemiological surveillance, drug prescriptions and infection management.

show abstract

“…Such variation in the proportion of ESBL-producers in different parts of the country may be attributable to various factors such as socio-economic conditions and common practice of drug misuse. Furthermore, eight (21.62%) isolates carried the bla CTX-M gene, which is lower than its prevalence reported in previous studies [47,50]. The distribution of ESBLs has evolved to a predominance of CTX-M enzymes, mainly with E. coli as one of the major carriers of ESBL encoding genes [51].…”

Section: Discussionmentioning

confidence: 70%

Efficacy of Urine Dipstick Test in Diagnosing Urinary Tract Infection and Detection of the blaCTX-M Gene among ESBL-Producing Escherichia coli

Gurung

Adhikari

et al. 2021

Diseases

View full text Add to dashboard Cite

A urine dipstick test used for prompt diagnosis of urinary tract infection (UTI) is a rapid and cost-effective method. The main objective of this study was to compare the efficacy of the urine dipstick test with culture methods in screening for UTIs along with the detection of the blaCTX-M gene in extended spectrum β-lactamase (ESBL)-producing Escherichia coli. A total of 217 mid-stream urine samples were collected from UTI-suspected patients attending Bharatpur Hospital, Chitwan, and tested by dipstick test strip (COMBI-10SL, Germany) prior to the culture. E. coli isolates were identified by standard microbiological procedures and subjected to antimicrobial susceptibility testing by Kirby Bauer disc diffusion method following CLSI guideline. Primary screening of ESBL-producing E. coli isolates was conducted using ceftriaxone, cefotaxime and ceftazidime discs and phenotypically confirmed by combined disk diffusion test. Plasmid DNA of ESBL-producing strains was extracted by phenol-chloroform method and subjected to PCR for detection of the blaCTX-M gene. Out of 217 urine samples, 48 (22.12%) showed significant bacteriuria. Among 46 (21.20%) Gram negative bacteria recovered, the predominant one was E. coli 37 (77.08%) of which 33 (89.19%) were multidrug resistant (MDR). E. coli isolates showed a higher degree of resistance towards cefazolin (62.16%) while 81.08% of the isolates were sensitive towards amikacin followed by nitrofurantoin (70.27%). Among 14 (37.84%) phenotypically confirmed ESBL isolates, only eight (21.62%) isolates carried the blaCTX-M gene. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of urine dipstick test were 43.75%, 77.51%, 35.59% and 82.91%, respectively. Besides, the use of dipstick test strip for screening UTI was associated with many false positive and negative results as compared to the gold standard culture method. Hence, dipstick nitrite test alone should not be used as sole method for screening UTIs.

show abstract

Isolation and molecular characteristics of extended spectrum beta-lactamase-producing uropathogenic Escherichia coli isolated from hospital attendees in Ebonyi State, Abakaliki

Cited by 3 publications

References 9 publications

Prevalence of the bla _CTX-M and bla _TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Prevalence of the bla _CTX-M and bla _TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Prevalence and Antibiogram Profile of Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae among Patients with Urinary Tract Infection in Abakaliki, Nigeria

Efficacy of Urine Dipstick Test in Diagnosing Urinary Tract Infection and Detection of the blaCTX-M Gene among ESBL-Producing Escherichia coli

Contact Info

Product

Resources

About

Isolation and molecular characteristics of extended spectrum beta-lactamase-producing uropathogenic Escherichia coli isolated from hospital attendees in Ebonyi State, Abakaliki

Cited by 3 publications

References 9 publications

Prevalence of the bla CTX-M and bla TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Prevalence of the bla CTX-M and bla TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Prevalence and Antibiogram Profile of Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae among Patients with Urinary Tract Infection in Abakaliki, Nigeria

Efficacy of Urine Dipstick Test in Diagnosing Urinary Tract Infection and Detection of the blaCTX-M Gene among ESBL-Producing Escherichia coli

Contact Info

Product

Resources

About

Prevalence of the bla _CTX-M and bla _TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Prevalence of the bla _CTX-M and bla _TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia