Isolation and molecular characterization of GP5 glycoprotein gene of Betaarterivirus suid 2 from Mizoram, India

Akter, Fatema; Roychoudhury, P.; Dutta, Tapan Kumar; Subudhi, P.K.; Kumar, Sanjeev; Gali, Jagan Mohanarao; Behera, Parthasarathi; Singh, Yengkhom Damodar

doi:10.1007/s13337-021-00735-x

Cited by 3 publications

(3 citation statements)

References 39 publications

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Section: Resultsmentioning

confidence: 99%

“…GP5 has multiple N-glycosylation sites, which is likely related to immune evasion by shielding neutralizing antibodies ( Akter et al, 2021 ; Zhu et al, 2022 ). There are five N-linked glycosylation sites in the amino acid position N 30 , N 32/33/34 , N 35 , N 44 , and N 51 of the GP5 glycoprotein of Betaarterivirus suid2 ( Akter et al, 2021 ). The potential N-glycosylation sites (NGSs) at N44 and N51 were conserved in all isolates, but the potential NGSs located upstream of N44 were relatively variable ( Fang et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…N-glycosylation of viral proteins is a complex post-translational modification that affects protein folding, viral entry, receptor interactions, immune escape and pathogenesis ( Das et al, 2011 ; Rupasinghe et al, 2022 ). GP5 contains five NGSs, including N 30 , N 32/33/34 , N 35 ,N 44 , and N 51 , which determine the antigenic properties and viral susceptibility to neutralizing antibodies ( Akter et al, 2021 ). The potential NGSs located upstream of N 44 were relatively variable while N 44 and N 51 are highly conserved in United States and European strains.…”

Section: Discussionmentioning

confidence: 99%

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Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022

et al. 2022

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Porcine reproductive and respiratory syndrome (PRRS) is an immunosuppressive disease caused by PRSS virus (PRRSV). PRRSV mainly causes reproductive disorders in pregnant sows and respiratory diseases in piglets. Recently, it has emerged as one of the most important diseases of the pig industry across the globe. In this study, we have collected 231 samples from differently sized pig farms in Eastern China from 2017 to 2022 to investigate the epidemic characteristics of the disease. All samples were screened by RT-PCR and analyzed further using Nsp2 and ORF5 genes. The result showed that the positive rate of PRRSV was 24% (54/231). Phylogenetic analysis (13 positive samples) revealed that all isolates belonged to genotype 2, and they were mainly distributed in four lineages (i.e., lineage 1, 3, 5, and 8). Nsp2 is the most variable protein among all PRRSV NSPs, several isolates from this study had amino acid deletions within Nsp2 compared to that of strain VR-2332. The major structural protein glycoprotein (GP5) protein is encoded by ORF5. Epitope analysis of the 13 isolated strains and additional reference strains revealed that all 13 strains had some mutations on the decoy epitope, the primary neutralizing epitope, T cell epitopes, and B cell epitopes. This study showed that the prevalent PRRSV strain in Eastern China was still HP-PRRSV, while the proportion of NADC30-like and NADC34-like strains have increased. This study further enriches the epidemiological data of PRRS in Eastern China and provides a theoretical basis for vaccine development and prevention and control of the disease across the region.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022

et al. 2022

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Research Progress on Glycoprotein 5 of Porcine Reproductive and Respiratory Syndrome Virus

Luo

Zheng

Zhang

et al. 2023

Animals

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Porcine reproductive and respiratory syndrome (PRRS) is an acute, febrile, and highly contagious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV). Glycoprotein 5 (GP5) is a glycosylated envelope protein encoded by the PRRSV ORF5, which has good immunogenicity and can induce the body to produce neutralizing antibodies. Therefore, study of GP5 protein is of great significance in the diagnosis, prevention, and control of PRRSV and the development of new vaccines. We reviewed GP5 protein genetic variation, immune function, interaction with viral protein and host proteins, induction of cell apoptosis, and stimulation of neutralizing antibodies. GP5 protein’s influence on virus replication and virulence, as well as its use as a target for viral detection and immunization are reviewed.

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Epidemiologic Investigation and Genetic Variation Analysis of PRRSV, PCV2, and PCV3 in Guangdong Province, China from 2020 to 2022

Yuan,

Sun,

Niu

et al. 2024

Viruses

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Recently, the emergence of HP-PRRSV (Highly Pathogenic porcine reproductive and respiratory syndrome virus) and the exacerbation of mixed infections of PRRSV and PCV have resulted in significant economic losses for the Chinese pig industry. This study collected a total of 226 samples suspected of infection with the aforementioned viruses from diverse pig farms in seven urban districts of central and northern Guangdong Province between 2020 and 2022. The positive rates of PRRSV, PCV2, and PCV3 in the samples were 33.2%, 37.6%, and 7.5%, respectively, and there were various mixed-infection scenarios present in the samples. This study successfully isolated multiple strains of PRRSV2 and PCV2 from their positive samples, and obtained the gene sequences of six PCV3 (ORF1 + ORF2) from samples. The associated sequences obtained were subjected to bioinformatic analysis and revealed the following:Predominantly prevalent strains of PRRSV in Guangdong Province include HP-PRRSV and NADC30-like variants, whereas PCV2 is primarily represented by the 2b and 2d subtypes. Specifically, the amino acid variation patterns exhibited by the PRRSV GP5 and NSP2 proteins of the strains sg_2108, qy_2008, and fs_2108 under environmental selective pressure are remarkably similar to the characteristics of Highly Pathogenic PRRSV; thus, it is inferred that they may possess higher virulence. The detected PCV3 strains were predominantly concentrated within the PCV3a-IM branch. All PRRSV strains involved in this study are wild-type-PRRSV (wt-PRRSV), comprising three recombinant strains and seven highly virulent strains. Among these strains, the ORF1a gene exhibited the highest variability in their genomes. Environmental selective pressure may enhance the virulence and immune evasion capabilities of PRRSV and drive mutations in the Cap proteins of PCV2 and PCV3. Conversely, PCV2 and PCV3 strains demonstrated greater stability in genetic evolution. In conclusion, this study enhances the epidemiological data regarding PRRSV, PCV2, and PCV3 in Guangdong Province, China, and is significant for the surveillance, prevention, and active control of these three diseases.

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Isolation and molecular characterization of GP5 glycoprotein gene of Betaarterivirus suid 2 from Mizoram, India

Cited by 3 publications

References 39 publications

Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022

Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022

Research Progress on Glycoprotein 5 of Porcine Reproductive and Respiratory Syndrome Virus

Epidemiologic Investigation and Genetic Variation Analysis of PRRSV, PCV2, and PCV3 in Guangdong Province, China from 2020 to 2022

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