Isolation and molecular characterization of Mycobacterium tuberculosis complex isolated from raw milk in some dairy farms in Egypt

Abstract

This study was conducted to detect Mycobacterium tuberculosis complex in milk in three Egyptian Governorates; ElSharkia, El-Menoufia and El-Behera Governorates. 300 milk samples were collected from tuberculin positive cases, 18 (6.0%) were shedding Mycobacterium tuberculosis complex in their milk which detected by real time PCR. On another hand, 170 milk samples were collected from tuberculin negative cases, 5 (2.9%) were shedding Mycobacterium tuberculosis complex in their milk which detected by real time PCR. All milk samples were examined by three techniques including Microscopic examination, culture and real time PCR. Real time PCR is more rapid and accurate method than microscopic and culture method. The isolated colonies from culture were examined by Multiplex PCR to demonstrate the source of infection either human or animal source.

Chemical and Bacteriological Assessment of Raw Milk Collected from Some Dairy Farms in El-Behera Governorate, Egypt.

“…In Egypt, similar prevalence of M. bovis in milk samples were detected as 3% and 2.5%from El-Sharkia and El -Behera Governorate [55] and [10], respectively. Lower results from Monufia Governorate (0.7%) [55].…”

“…Methicillin-resistant S. aureus has been identified in a variety of livestock animals, with the highest prevalence observed in pigs, fattening calves, and turkeys as well as dairy cattle herds, where they pose an additional threat to animal health by causing subclinical and clinical mastitis [8,9]. higher in some regions and countries than in others, particularly where there is a close relationship between the number of cattle and the population and where milk and dairy products are often consumed unpasteurized [10,11].…”

Estudo da interferência de substratos orgânicos na ação do ozônio sobre microrganismos deteriorantes, benéficos e patogênicos

“…Mycobacterium tuberculosis é o agente etiológico da tuberculose humana, considerada a principal causa de morte em adultos por um único agente infeccioso, sendo responsável por 26% das mortes passíveis de prevenção no mundo. Não se conhece a proporção de casos de tuberculose causada por Mycobacterium bovis(CASANOVA e ABEL, 2002, HUSSIEN e MAHROUS, 2016.O gênero Staphylococcus é constituído de 40 espécies e 24 subespécies(MURRAY, 2010). A morfologia típica do gênero é classificada em cocos apresentando diâmetro médio de 1 µm e que tendem a se agrupar em arranjos semelhantes a cachos de uva, apresenta necessidade nutricional complexa.…”