Isolation and molecular characterization of some copper biosorped strains

Abstract

ABSTRACT:The biosorption of copper (II) from aqueous solution using different bacterial strains was studied.Copper-biosorbing bacteria were isolated from tannery effluent collected from Borg Al-Arab, Alexandria, Egypt. These isolates displayed different degrees of copper biosorption under aerobic conditions. Based on 16S rDNA gene sequence analysis, three of them (S2, S5 and S7) were identified as Chryseobacterium sp., Enterobacter sp. and Stenotrophomonas sp., respectively. Initial copper (II) ion concentrations from 25-250 mg/L at constant temperature 30 ºC were studied. The residual copper (II) concentration and its toxicity effect in solution were determined using atomic absorption spectrophotometer and bioluminescent bioreporter. The bioluminescence inhibition of strain (S5) reached to 91.4 % as compared with the strain (S7) reached to 83.3 % at 225 mg/L of copper ion where the maximum biosorption efficiency for S5 and S7 were 71 % and 70.1 % correspondingly using atomic absorption. The bioluminescent bioreporter was proved to be fast and accurate technique for measurement the toxicity effect of residual copper (II) in solution.

Molecular and Physiological Characterization of Copper-Resistant Bacteria Isolated from Activated Sludge in an Industrial Wastewater Treatment Plant in Rungkut-Surabaya, Indonesia

“…Conventional techniques for removing dissolved heavy metals include chemical precipitation, carbon adsorption, electrolytic recovery, ion-exchange, chelation and solvent extraction or liquid membrane separationall exhibit several disadvantages, such as high cost, incomplete removal, low selectivity, high energy consumption and generation of toxic slurries that are difficult to be eliminated. Therefore, much attention has been paid to the removal of metal ions by microorganisms due to its potential applications in environmental protection and recovery of toxic heavy metals (Zaki and Farag 2010).…”

Bioremediation of Hexavalent Chromium Widely Discharged in Leather Tanning Effluents

“…Among those, reverse osmosis, precipitation, chemical reduction followed by precipitation, ion exchange and absorption on coal, activated carbon, alum or kaolinite, are employed [27.28]. The major disadvantages of these techniques apart from being economically expensive is the incomplete metal removal, high reagent and energy requirements, and generation of toxic sludge or other waste products which require disposal [29][30][31].…”