Isolation and Molecular Characterization of Gelatinase-Producing Bacteria from Mangrove Sediment

Abstract

Protease is an important enzyme widely produced by microorganisms applied in food, health, and industry. Mangrove ecosystem, a rich microorganism habitat, accounted as a new resource for isolating the proteolytic bacteria. The purpose of this study was to identify protease-producing bacteria from mangrove ecosystems in the Tuban area, Indonesia. Three isolates that produced the gelatinase was successfully isolated from mangrove sediments. Bacterial isolates were then tested for extracellular gelatinase. The results showed that isolate T1 had high gelatinase activity. Two isolates (isolates T2 and T3) produced moderately gelatinase enzymes. Molecular identification revealed that isolate T1 is Enterobacter hormaechei.

Molecular identification of thermophilic bacteria with antimicrobial activity isolated from hot springs in North Sumatra, Indonesia

“…Furthermore, the culture media was stored in the refrigerator for 15 min. A positive gelatinase test was indicated by the form of the medium, which remains liquid even when stored in the refrigerator (Prihanto et al 2020).…”