Isolation and Molecular Characterization of Two Lectins from Dwarf Elder (Sambucus ebulus L.) Blossoms Related to the  Sam n1 Allergen

Jiménez, Pilar; Cabrero, Patricia; Basterrechea, José E.; Tejero, Jesús; Córdoba-Díaz, Damián; Girbés, Tomás

doi:10.3390/toxins5101767

Cited by 17 publications

(18 citation statements)

References 30 publications

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“…We have previously reported that oral ingestion of ebulin f at 5 mg/kg of body weight killed 50% of the animals in 10 days, while those that survived recovered after 30 days from the administration of the toxin [ 10 ]. We obtained similar results following nasal administration of ebulin blo at the same dose [ 18 ]. We estimated, therefore, an approximate LD 50 of 5 mg/kg of body weight for both oral and nasal administration, in contrast to the LD 50 value of 2.8 mg/kg body weight for i.p.…”

Section: Introductionmentioning

confidence: 59%

“…Previous studies have described the toxicity of ebulin f and ebulin blo in six-week-old mice [ 11 , 18 ]. Early intestinal targets were the transit amplifying cells present in the small intestine crypts, as well as the cells of the large intestine crypts.…”

Section: Introductionmentioning

confidence: 99%

“…In both cases, the cellular action mechanism was the promotion of apoptosis [ 11 ]. The presence of these proteins in certain food products, the concern regarding their possible criminal use or as a bioweapon and also their potential application in medicine make the toxicology of these proteins an area of great interest [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Toxicity of the Anti-ribosomal Lectin Ebulin f in Lungs and Intestines in Elderly Mice

Garrosa

Jiménez

Tejero

et al. 2015

Toxins

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All parts of dwarf elder (Sambucus ebulus L.) studied so far contain a ribosome-inactivating protein with lectin activity (ribosome-inactivating lectin; RIL), known as ebulin. Green fruits contain ebulin f, the toxicity of which has been studied in six-week-old mice, where it was found that the intestines were primary targets for it when administered intraperitoneally (i.p.). We performed experiments to assess whether ebulin f administration to six- and 12-month-old mice would trigger higher toxicity than that displayed in six-week-old mice. In the present report, we present evidence indicating that the toxicological effects of ebulin f after its i.p. administration to elderly mice are exerted on the lungs and intestines by an increased rate of apoptosis. We hypothesize that the ebulin f apoptosis-promoting action together with the age-dependent high rate of apoptosis result in an increase in the lectin’s toxicity, leading to a higher lethality level.

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Section: Introductionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

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Toxicity of the Anti-ribosomal Lectin Ebulin f in Lungs and Intestines in Elderly Mice

Garrosa

Jiménez

Tejero

et al. 2015

Toxins

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“…Since, as will be indicated below, ebulin f displays a significant oral toxicity in mice, it is important to reduce the influence of ebulin f without affecting its antioxidant capacity. A short heating of ripe fruit extracts only reduced the content of total phenols and the antioxidant and free-scavenging activities by up to 13.5% but completely sensitized ebulin f to pepsin [ 36 ].…”

Section: Isoforms Of Ebulinmentioning

confidence: 99%

“…Dwarf elder blossoms also contain an isoform of ebulin, which we named ebulin blo, together with a homodimeric lectin we called SELblo [ 36 ]. MALDI-TOF mass spectrometry analysis of the native protein revealed a molecular mass at m/z of 63,225.…”

Section: Isoforms Of Ebulinmentioning

confidence: 99%

Ebulin from Dwarf Elder (Sambucus ebulus L.): A Mini-Review

Jiménez

Tejero

Córdoba-Díaz

et al. 2015

Toxins

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Sambucus ebulus L. (dwarf elder) is a medicinal plant, the usefulness of which also as food is restricted due to its toxicity. In the last few years, both the chemistry and pharmacology of Sambucus ebulus L. have been investigated. Among the structural and functional proteins present in the plant, sugar-binding proteins (lectins) with or without anti-ribosomal activity and single chain ribosome-inactivating proteins (RIPs) have been isolated. RIPs are enzymes (E.C. 3.2.2.22) that display N-glycosidase activity on the 28S rRNA subunit, leading to the inhibition of protein synthesis by arresting the step of polypeptide chain elongation. The biological role of all these proteins is as yet unknown. The evidence suggests that they could be involved in the defense of the plant against predators and viruses or/and a nitrogen store, with an impact on the nutritional characteristics and food safety. In this mini-review we describe all the isoforms of ebulin that have to date been isolated from dwarf elder, as well as their functional characteristics and potential uses, whilst highlighting concern regarding ebulin toxicity.

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Leftover and weed, joint efforts to preserve health: Joke or reality?

Kurćubić,

Stajić,

Živković

et al. 2024

Future Postharvest and Food

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This review sheds light on opportunities to transition to a sustainable food system by reducing the use of synthetic additives, preventing biodiversity loss, and creating healthy foods with the most desirable biological and nutritional value in accordance with the ONE HEALTH approach, positively affecting the preservation of the health of animals, people and the environment. The promotion of the value of the circular economy is reflected in the use of a by‐product of the winery (grape pomace – GP) and weed (Sambucus ebulus L. ‐ Dwarf Elder (DE)) for the production of bioactive substances (BAS) from which phytocomplexes (PHYCO) will be created for various purposes in achieving the ultimate goal: healthier human population. We comprehensively analyzed the benefits of the possibility that the addition of PHYCO of plant extracts and lyophilizates (LYOP) in food/feed enables the highest concentration of BAS while the doses of PHYCO are the lowest/cheapest. Such bioproduction must enable the creation of supplements (antimicrobials/antioxidants) or food with desirable techno‐functional and sensory properties and preventive/therapeutic action.

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Isolation and Molecular Characterization of Two Lectins from Dwarf Elder (Sambucus ebulus L.) Blossoms Related to the Sam n1 Allergen

Cited by 17 publications

References 30 publications

Toxicity of the Anti-ribosomal Lectin Ebulin f in Lungs and Intestines in Elderly Mice

Toxicity of the Anti-ribosomal Lectin Ebulin f in Lungs and Intestines in Elderly Mice

Ebulin from Dwarf Elder (Sambucus ebulus L.): A Mini-Review

Leftover and weed, joint efforts to preserve health: Joke or reality?

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