Isolation and Molecular Characterization of the Transformer Gene From Bactrocera cucurbitae (Diptera: Tephritidae)

Luo, Ya; Zhao, Santao; Li, Jia-hui; Li, Peizheng; Yan, Rihui

doi:10.1093/jisesa/iex031

Cited by 9 publications

(8 citation statements)

References 45 publications

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“…In B. dorsalis , the tra homologue Bdtra serves as a genetic switch in early embryonic development via the alternative sex‐specific splicing cascade to determine the sexual fate . The genomic structure and sex‐specific splicing pattern of Bdtra are similar to those found in other tephritid pests, such as Ceretitis capitata , B. oleae , Anastrepha oblique , Anastrepha suspensa , B. tryoni and B. jarvisi and B. cucurbitae . Moreover, disturbance of the tra gene mediated by RNA interference in early embryonic development resulted in sexual reversal from an XX female to an XX pseudomale, suggesting possible application in pest control.…”

Section: Introductionmentioning

confidence: 56%

“…The wild‐type (WT) oriental fruit flies used in this study were originally collected from Haidian campus of Hainan University and cultured in the laboratory at 26 °C 70% relative humidity (RH), under a 14 : 10 h light/dark photoperiod. Adults were fed with medium comprising brewer's yeast powder/cane sugar (1 : 2 w/w), and larvae were maintained on an artificial diet . Before pupation, the grown larvae were transferred to plastic boxes containing wet sand.…”

Section: Methodsmentioning

confidence: 99%

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Efficient somatic and germline genome engineering of Bactrocera dorsalis by the CRISPR/Cas9 system

Zhao

Xing

Liu

et al. 2019

Pest Management Science

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BACKGROUND: Bactrocera dorsalis (Hendel), a very destructive insect pest of many fruits and vegetables, is widespread in many Asian countries. To facilitate control of this pest, it is essential to investigate its genetics and gene function using targeted gene disruption.RESULTS: Here, we describe successful targeted mutagenesis of the white and transformer genes in B. dorsalis through use of the clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) system. Co-injection of the white sgRNA and Cas9 mRNA into B. dorsalis embryos caused eye color change, and the white mutations in the germline were heritable. CRISPR-mediated knockout of the sex determination gene transformer (tra) in B. dorsalis resulted in a male-biased sex ratio and adult flies with abnormal outer and interior reproductive organs. Small indels and substitutions were induced by CRIRPR for both genes.CONCLUSION: Our data demonstrate that somatic and germline genome engineering of the pest B. dorsalis can be performed efficiently using the CRISPR/Cas9 system, opening the door to the use of the CRISPR-mediated method for functional annotations of genes in B. dorsalis and for its population control using, for example, such as gene drive.

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Section: Introductionmentioning

confidence: 56%

Section: Methodsmentioning

confidence: 99%

Efficient somatic and germline genome engineering of Bactrocera dorsalis by the CRISPR/Cas9 system

Zhao

Xing

Liu

et al. 2019

Pest Management Science

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“…The structure of Zttra was similar to the structures of orthologs in dipterans, especially in tephritid species. Previous studies have shown that tra is sex‐specifically regulated by alternative splicing (Boggs et al ., 1987; Peng et al ., 2015; Luo et al ., 2017). Two male Zttra transcripts ( Zttra ‐M1 and Zttra ‐M2), which included two and three male‐specific exons, respectively, were detected.…”

Section: Discussionmentioning

confidence: 99%

Early embryonic transcriptomes of Zeugodacus tau provide insight into sex determination and differentiation genes

Zheng

Qiao

et al. 2021

Insect Science

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Zeugodacus tau (Walker) is an invasive pest. The sterile insect technique is an environment‐friendly method for pest control. Understanding the mechanism of sex determination will contribute to improving efficiency of this technique. In this study, we identified the transformer (tra) gene in Z. tau. One female‐specific and two male‐specific isoforms of tra were found in Z. tau, and the male‐specific splicing pattern of tra was found to occur 5 h after egg laying. We performed transcriptome sequencing at 1 h (E1), 5 h (E5), and 9 h (E9) after egg laying and obtained high‐quality transcriptome libraries of early embryo development. We identified 13 297 and 11 713 differentially expressed genes (DEGs) from E5 versus E1 and E9 versus E1 comparisons, respectively. To explore the potential functions of the DEGs during embryonic development, Gene Ontology, Clusters of Orthologous Groups of proteins, and Kyoto Encyclopedia of Genes and Genomes analyses were performed. Twenty‐six genes potentially involved in sex determination or differentiation, including Maleness‐on‐the‐Y (MoY), were identified in Z. tau. To verify the transcriptome results, 15 genes were selected for quantitative real‐time PCR validation. The results were consistent with the transcriptome sequencing results. Moreover, U2 small nuclear riboprotein auxiliary factor (U2AF‐50), female lethal d (fl(2)d), and virilizer (vir) were highly expressed at E5, indicating that they may be related to the sex‐specific splicing of tra. Further functional analysis is needed to confirm this speculation. Our data provide an insight into the mechanism underlying sex determination and differentiation in tephritid species.

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“…Despite the existence of general principles of female and male development, different species show diverse primary sex determination signals (Beye et al, 2003;Cline, 1979;Cline & Meyer, 1996;Hall et al, 2015;Krzywinska et al, 2016;Meccariello et al, 2019;Sanchez, 2010;Sharma et al, 2017). A common theme is that diverse primary signals directly or indirectly regulate the sex-specific expression of the dsx gene repeatedly via transformer (tra) homologous genes (Concha & Scott, 2009;Gempe et al, 2009;Geuverink, Kraaijeveld, et al, 2018a;Geuverink, Verhulst, et al, 2018b;Gotoh et al, 2016;Hasselmann et al, 2008;Hediger et al, 2010;Jia et al, 2016;Lagos et al, 2007;Li et al, 2013;Liu et al, 2015;Luo et al, 2017;Morita et al, 2019;Morrow et al, 2014;Pane et al, 2002;Petrella et al, 2019;Ruiz et al, 2007;Schetelig et al, 2012;Shukla & Palli, 2012;Suzuki et al, 2015;Tian & Maniatis, 1993;Verhulst et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Sexual diversification of splicing regulation during embryonic development in honeybees (Apis mellifera), A haplodiploid system

Netschitailo

Raub

Kaftanoğlu

et al. 2021

Insect Molecular Biology

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The honeybee is a haplodiploid organism in which sexual development is determined by the complementary sex determiner (csd) gene and realized by sex-specific splicing processes involving the feminizer (fem) gene. We used high throughput transcriptome sequencing (RNA-Seq) to characterize the transcriptional differences between the sexes caused by the fertilization and sex determination processes in honeybee (Apis mellifera) embryos. We identified 758, 372 and 43 differentially expressed genes (DEGs) and 58, 176 and 233 differentially spliced genes (DSGs) in 10-15-h-old, 25-40-h-old and 55-70-h-old female and male embryos, respectively. The early difference in male and female embryos in response to the fertilization and non-fertilization processes resulted mainly in differential expression of genes (758 DEGs vs. 58 DSGs). In the latest sampled embryonic stage, the transcriptional differences between the sexes were dominated by alternative splicing of transcripts (43 DEGs vs. 233 DSGs). Interestingly, differentially spliced transcripts that encode RNAbinding properties were overrepresented in 55-70-h-old embryos, indicating a more diverse regulation via alternative splicing than previous work on the sex determination pathway suggested. These stage-and sex-specific transcriptome data from honeybee embryos provide a comprehensive resource for examining the roles of fertilization and sex determination in developmental programming in a haplodiploid system.

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Isolation and Molecular Characterization of the Transformer Gene From Bactrocera cucurbitae (Diptera: Tephritidae)

Cited by 9 publications

References 45 publications

Efficient somatic and germline genome engineering of Bactrocera dorsalis by the CRISPR/Cas9 system

Efficient somatic and germline genome engineering of Bactrocera dorsalis by the CRISPR/Cas9 system

Early embryonic transcriptomes of Zeugodacus tau provide insight into sex determination and differentiation genes

Sexual diversification of splicing regulation during embryonic development in honeybees (Apis mellifera), A haplodiploid system

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