Isolation and molecular characterization of antifungal metabolite producing actinomycete from mangrove soil

Palla, Mary Sulakshana; Guntuku, Girija Shankar; Muthyala, Murali Krishna Kumar; Pingali, Sirisha; Sahu, Prafulla Kumar

doi:10.1016/j.bjbas.2018.02.006

Cited by 28 publications

(24 citation statements)

References 24 publications

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“…Cross streak method is the widely utilized primary screening method for determining the antagonistic role of bacteria ( Palla et al, 2018 ). This method establishes foundation for secondary screening process.…”

Section: Discussionmentioning

confidence: 99%

In vitro biological properties of Streptomyces cangkringensis isolated from the floral rhizosphere regions

Saraswathi¹,

Mahalakshmi²,

Khusro³

et al. 2020

Saudi Journal of Biological Sciences

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This context was investigated to determine in vitro antimicrobial, antioxidative, and anticancer traits of crude ethyl acetate extract of Streptomyces cangkringensis strain TSAS 04 isolated from soil sample of rhizosphere regions. The antimicrobial activity of ethyl acetate extract of strain TSAS 04 was determined against indicator pathogens using disc diffusion assay which exhibited maximum zones of inhibition of 20.6 ± 0.3 and 16.3 ± 0.6 mm against Bacillus subtilis and Trichoderma viride , respectively. In vitro antioxidant properties of the crude ethyl acetate extract were performed using standard methodologies. The extract revealed maximum DPPḢ and ABTS •+ radical scavenging activities of 51.1 ± 0.39 and 81.25 ± 0.33%, respectively. Likewise, maximum phosphomolybdenum reduction and Fe 3+ reduction of the crude ethyl acetate extract of strain TSAS 04 were estimated 76.18 ± 0.10 and 89.01 ± 0.44%, respectively. In vitro anticancer trait of the extract was determined against HeLa cell line using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay which showed anticancer activities in a dose dependent manner with an IC 50 value of 410.5 µg/mL. Fourier transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectrometry (GC-MS) analyses indicated the presence of distinct functional groups and bioactive components in the extract, respectively. In conclusion, S. cangkringensis strain TSAS 04 showed its effectiveness as ideal bioactive agent by exhibiting substantial antimicrobial, antioxidant, and anticancer properties.

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Section: Discussionmentioning

confidence: 99%

In vitro biological properties of Streptomyces cangkringensis isolated from the floral rhizosphere regions

Saraswathi¹,

Mahalakshmi²,

Khusro³

et al. 2020

Saudi Journal of Biological Sciences

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“…Saboraud's agar plate was streaked with selected fungi (C. albicans, A. niger and P. notatum) as per standard protocol (Palla et al, 2018). 100 μl dose of 3 % P. granatum aqueous and alcoholic extracts solutions and standard drug Fluconazole in concentration 0.5 % was loaded per bore respectively in aseptic condition with help of sterile micropipette.…”

Section: Anti-dandruff Activitymentioning

confidence: 99%

“…100 μl dose of 3 % P. granatum aqueous and alcoholic extracts solutions and standard drug Fluconazole in concentration 0.5 % was loaded per bore respectively in aseptic condition with help of sterile micropipette. Plates were kept at room temperature for 30 min and then incubated at 37 C for 24 h (Palla et al, 2018). The diameter of the zones of inhibition was measured with the help of scale in mm (Chavan et al, 2020b;Bhinge et al, 2020).…”

Section: Anti-dandruff Activitymentioning

confidence: 99%

Screening of hair growth promoting activity of Punica granatum L. (pomegranate) leaves extracts and its potential to exhibit antidandruff and anti-lice effect

Bhinge¹,

Bhutkar²,

Randive³

et al. 2021

Heliyon

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The intent of the present investigation was to explore the utility of alcoholic and aqueous extract of Punica granatum L. as hair growth promoter along with anti-lice and antidandruff activity. A filter paper diffusion approach was employed for screening of the pediculocidal and ovicidal activity. Albino mice, preselected for their telogen phase of hair growth were used during the study. The prepared extracts, Minoxidil and control were applied over shaved skin surface on to the backs of mice to assess telogen to anagen transition. The qualitative and quantitative analysis was performed. The outcome of the studies revealed that Punica granatum L. alcoholic and aqueous extracts exhibited prominent anti-lice activity. The transition of telogen to anagen phase of the number of anagen hair follicle was observed in approximately 45, 27 and 51% of animals treated with alcoholic and aqueous extract of Punica granatum L., and Minoxidil, respectively, which suggest the hair growth promoting potential of the extract of Punica granatum L. Also, 3 % Punica granatum L. alcoholic extracts exhibited a potent antidandruff activity against fungal strains tested. Maltol, was identified as a principal phytoconstituent in the alcoholic extract. The findings greatly suggest anti-lice, antidandruff and hair growth promoting potential of the extract of Punica granatum L.

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“…Fungi isolated from mangrove sediments received much attention due to structural diversity, unique biological activity and functionally active secondary metabolites 5,6 . The fungi from the mangrove habitats produce novel bioactive metabolites including antibiotic, anti-diabetic, antiviral, anti-inflammatory, anticancer, antioxidant, and immuno-suppressive drugs 7,8 .…”

Section: Ijpsr (2020) Volume 11 Issue 7 (Research Article)mentioning

confidence: 99%

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2020

IJPSR

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The intention of the present study was to isolate and identify cultural conditions of potent fungal strain VJCH-19 with antagonistic activities isolated from mangrove ecosystems of Adavuladeevi, South coast, Andhra Pradesh, India. Methods: The soil samples were collected from the mangrove habitats and shade dried. The serial dilution plate technique was employed for the isolation of mangrove fungi by using Sabouraud dextrose agar and Czapek-Dox agar media. All the strains were screened for the production of antimicrobial compounds. The potential fungal strain was designated as VJCH-19. The identification of the strain was carried out based on cultural, micromorphological and genomic characteristics. Results: The potent mangrove fungal strain VJCH-19 was identified as Simplicillium lanosoniveum. The secondary metabolites produced by the strain inhibited several bacteria and fungi. To check the nutrient adaptation, VJCH-19 was grown on different culture media and its morphological characters were observed. Conclusion: Among the twenty-five fungal strains, VJCH-19 was found to possess antimicrobial potential and the strain was identified as Simplicilium lanosoniveum VJCH-19. INTRODUCTION: Mangroves are salt-tolerant forest ecosystems confined to intertidal zones of sheltered shores, estuaries, tidal creeks, backwaters, lagoons, marshes, and mudflats of the tropical and subtropical latitudes 1. Mangrove soils provide a unique ecological niche for the growth of diversified microorganisms which find use in recycling environmental nutrients and production of exclusive secondary metabolites of industrial importance 2, 3, 4 .

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Isolation and molecular characterization of antifungal metabolite producing actinomycete from mangrove soil

Cited by 28 publications

References 24 publications

In vitro biological properties of Streptomyces cangkringensis isolated from the floral rhizosphere regions

In vitro biological properties of Streptomyces cangkringensis isolated from the floral rhizosphere regions

Screening of hair growth promoting activity of Punica granatum L. (pomegranate) leaves extracts and its potential to exhibit antidandruff and anti-lice effect

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