Isolation and molecular characterization of a cryptic plasmid from Bifidobacterium longum

Kim, Kihwan; Park, Ju Hui; Oh, Inkyung; Yoon, Sang Hyun

doi:10.1007/s10529-007-9504-5

Cited by 17 publications

(21 citation statements)

References 23 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of shuttle cloning vectors for bifidobacteria with E. coli plasmid replicons have been constructed, and electroporation procedures for bifidobacteria have been developed (4,162,177,178,188,222,225,254,255,268,287). The highcopy-number ColE1-based replication origin of E. coli was used for the construction of all except one of the E. coliBifidobacterium shuttle cloning vectors (4,177,178,225,255,287,309).…”

Section: Development Of Cloning and Expression Vector Systems For Bifmentioning

confidence: 99%

Genomic Insights into Bifidobacteria

Lee

O’Sullivan

2010

Microbiol Mol Biol Rev

249

197

View full text Add to dashboard Cite

SUMMARY Since the discovery in 1899 of bifidobacteria as numerically dominant microbes in the feces of breast-fed infants, there have been numerous studies addressing their role in modulating gut microflora as well as their other potential health benefits. Because of this, they are frequently incorporated into foods as probiotic cultures. An understanding of their full interactions with intestinal microbes and the host is needed to scientifically validate any health benefits they may afford. Recently, the genome sequences of nine strains representing four species of Bifidobacterium became available. A comparative genome analysis of these genomes reveals a likely efficient capacity to adapt to their habitats, with B. longum subsp. infantis exhibiting more genomic potential to utilize human milk oligosaccharides, consistent with its habitat in the infant gut. Conversely, B. longum subsp. longum exhibits a higher genomic potential for utilization of plant-derived complex carbohydrates and polyols, consistent with its habitat in an adult gut. An intriguing observation is the loss of much of this genome potential when strains are adapted to pure culture environments, as highlighted by the genomes of B. animalis subsp. lactis strains, which exhibit the least potential for a gut habitat and are believed to have evolved from the B. animalis species during adaptation to dairy fermentation environments.

show abstract

Section: Development Of Cloning and Expression Vector Systems For Bifmentioning

confidence: 99%

Genomic Insights into Bifidobacteria

Lee

O’Sullivan

2010

Microbiol Mol Biol Rev

249

197

View full text Add to dashboard Cite

show abstract

“…The replicon of plasmid pMB1 was shown to replicate stably in B. longum, B. animalis, and B. adolescentis by the theta mechanism and was used to express endostatin, tumor necrosis factor (TNF)-related apoptosisinducing ligand, and human interleukin 10 (19, 28, 41, 68, 71, 86). The replicon of the theta-replicating plasmid pMG1 has been used exclusively in its native host strain B. longum MG1 for expression of the rice glutamate decarboxylase, the bacteriocin pediocin, and the cholesterol oxidase of Streptomyces coelicolor (52,59,61,62). The theta-type replicon of pBC1 has been shown to replicate stably in B. pseudocatenulatum, B. breve, and B. longum subsp.…”

Section: Plasmidsmentioning

confidence: 99%

Accessing the Inaccessible: Molecular Tools for Bifidobacteria

Sun

Baur

Zhurina

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

ABSTRACTBifidobacteria are an important group of the human intestinal microbiota that have been shown to exert a number of beneficial probiotic effects on the health status of their host. Due to these effects, bifidobacteria have attracted strong interest in health care and food industries for probiotic applications and several species are listed as so-called “generally recognized as safe” (GRAS) microorganisms. Moreover, recent studies have pointed out their potential as an alternative or supplementary strategy in tumor therapy or as live vaccines. In order to study the mechanisms by which these organisms exert their beneficial effects and to generate recombinant strains that can be used as drug delivery vectors or live vaccines, appropriate molecular tools are indispensable. This review provides an overview of the currently available methods and tools to generate recombinant strains of bifidobacteria. The currently used protocols for transformation of bifidobacteria, as well as replicons, selection markers, and determinants of expression, will be summarized. We will further discuss promoters, terminators, and localization signals that have been used for successful generation of expression vectors.

show abstract

“…These included, among others, general E. coli-Bifidobacterium shuttle vectors (1,5,18,21,25,26,30,35,37), replicon screening vectors (12), and expression vectors (40). However, the majority of these are based on poorly characterized replicons since the mode of replication has been investigated for only a few plasmids (4,21,27,32). Indeed, apart from pBC1 (2), only the recently reported plasmid pCIBA089 from Bifidobacterium asteroides has been characterized at the molecular level (5).…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, 14 fully sequenced plasmids from eight bifidobacterial species are reported in the GenBank database (http://www.ncbi.nlm.nih.gov/sites/entrez). However, the basic biology of plasmids in this genus remains poorly understood; indeed, the mode of replication has been analyzed for only a few of them (4,21,27,30,32). Furthermore, the dissection of the open reading frames and the analysis of untranslated sequences and structures has been undertaken for only a couple of plasmids (2,5).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Improved Cloning Vectors for Bifidobacteria, Based on the Bifidobacterium catenulatum pBC1 Replicon

Álvarez‐Martín

Flórez

Margollés

et al. 2008

Appl Environ Microbiol

View full text Add to dashboard Cite

This study reports the development of several cloning vectors for bifidobacteria based on the replicon of pBC1, a cryptic plasmid from Bifidobacterium catenulatum L48 thought to replicate via the theta mode. These vectors, in which antibiotic resistance genes encoding either erythromycin or tetracycline resistance acted as selection markers, were able to replicate in a series of eight Bifidobacterium species at frequencies ranging from 4.0 ؋ 10 1 to 1.0 ؋ 10 5 transformants g ؊1 but not in Lactococcus lactis or Lactobacillus casei. They showed a relative copy number of around 30 molecules per chromosome equivalent and a good segregational stability, with more than 95% of the cells retaining the vectors after 80 to 100 generations in the absence of selection. Vectors contain multiple cloning sites of different lengths, and the lacZ␣ peptide gene was introduced into one of the molecules, thus allowing the easy selection of colonies harboring recombinant plasmids in Escherichia coli. The functionality of the vectors for engineering Bifidobacterium strains was assessed by cloning and examining the expression of an ␣-L-arabinofuranosidase gene belonging to Bifidobacterium longum. E. coli and Bifidobacterium pseudocatenulatum recombinant clones were stable and showed an increase in ␣-arabinofuranosidase activity of over 100-fold compared to that of the untransformed hosts.

show abstract

Isolation and molecular characterization of a cryptic plasmid from Bifidobacterium longum

Cited by 17 publications

References 23 publications

Genomic Insights into Bifidobacteria

Genomic Insights into Bifidobacteria

Accessing the Inaccessible: Molecular Tools for Bifidobacteria

Improved Cloning Vectors for Bifidobacteria, Based on the Bifidobacterium catenulatum pBC1 Replicon

Contact Info

Product

Resources

About