Isolation and molecular characterization of beta-lactamase-producing Haemophilus parainfluenzae from the genital tract

Martel, Annie; Gosselin, P; Ouellette, Marc; Roy, Paul H.; Bergeron, Michel G.

doi:10.1128/aac.31.6.966

Cited by 9 publications

(8 citation statements)

References 17 publications

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“…After the recognition of plasmid-mediated beta-lactamase in gonococci, it was speculated that Haemophilus parainfluenzae may have been the plasmid source, or at least the host immediately prior to the gonococcus (180). Recently, we have seen what may be transfer in the other direction, with gonococcal-like plasmids reported in H. parainfluenzae (122).…”

Section: Legionella Sppmentioning

confidence: 99%

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Mayer

1988

Clin Microbiol Rev

152

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Plasmids are circular deoxyribonucleic acid molecules that exist in bacteria, usually independent of the chromosome. The study of plasmids is important to medical microbiology because plasmids can encode genes for antibiotic resistance or virulence factors. Plasmids can also serve as markers of various bacterial strains when a typing system referred to as plasmid profiling, or plasmid fingerprinting is used. In these methods partially purified plasma deoxyribonucleic acid species are separated according to molecular size by agarose gel electrophoresis. In a second procedure, plasmid deoxyribonucleic acid which has been cleaved by restriction endonucleases can be separated by agarose gel electrophoresis and the resulting pattern of fragments can be used to verify the identity of bacterial isolates. Because many species of bacteria contain plasmids, plasmid profile typing has been used to investigate outbreaks of many bacterial diseases and to trace inter- and intra-species spread of antibiotic resistance.

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Section: Legionella Sppmentioning

confidence: 99%

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Mayer

1988

Clin Microbiol Rev

152

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“…The bacterium is notorious for β-lactamase production, and its β-lactamase producing ability is typically mediated by transferable plasmids. 17,[24][25][26] H. parainfluenzae is also considered as the origin and reservoir for the dissemination of β-lactamase-carrying plasmids to other bacterial species. 27 The β-lactamases detected include the TEM-1 and TEM-15 types, which are usually associated with plasmids.…”

Section: β-Lactam Antibioticsmentioning

confidence: 99%

“…14,16,28,29 It was reported that the plasmid that carried the TEM-1 β-lactamase was identical to one isolated from 'Africantype' penicillinase-producing Neisseria gonorrhoeae strain. 26,30 Inhibitor resistant β-lactamases TEM-34 and TEM-182, which were both carried by TnA transposon of the Tn2 type, were also detected in H. parainfluenzae in 2011. 16 β-lactam resistance in H. parainfluenzae not mediated by β-lactamases has also been reported.…”

Section: β-Lactam Antibioticsmentioning

confidence: 99%

Mechanisms of antibiotic resistance inHaemophilus parainfluenzae

Abotsi

Govinden

Essack

2017

Southern African Journal of Infectious Diseases

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Haemophilus parainfluenzae is part of the HACEK group of fastidious bacteria commonly implicated in endocarditis and bacteremia. Previously considered as a normal respiratory, oral and sometimes genitourinary commensal, it has been recognised as a pathogen that can cause life-threatening infections in both immunocompromised and healthy individuals. It has also been reported as a bacterium that can harbor transferable antibiotic resistance genes. This paper presents a literature review on the molecular mechanisms of resistance of H. parainfluenzae to commonly prescribed antibiotics and discusses areas for further research.

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“…They can be mobilized by the 24.5-megadalton (MDa) indigenous conjugative gonococcal plasmid, conjugative Haemophilus plasmids, and enteric plasmids (9,10,14,15,19). The 3.2-MDa 3-lactamase plasmid has also been associated with strains of Haemophilus ducreyi and urogenital Haemophilus parainfluenzae (1,13). In contrast, the ,-lactamase plasmid was maintained in only a few strains of Neisseriaflava, Neisseria cinerea, Neisseria subflava, Neisseria mucosa, and N. subflava-N. sicca but not in strains of Neisseria meningitidis under laboratory conditions (8).…”

mentioning

confidence: 99%

Transfer of beta-lactamase plasmids from Neisseria gonorrhoeae to Neisseria meningitidis and commensal Neisseria species by the 25.2-megadalton conjugative plasmid

Roberts

Knapp

1988

Antimicrob Agents Chemother

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Two highly tetracycline-resistant, ,-lactamase-producing Neisseria gonorrhoeae strains were used as donors for conjugation with Neisseria meningitidis and commensal Neisseria species. We found that both strains were able to transfer the 4.4-and 3.2-megadalton ,-lactamase plasmids to the recipients tested, with frequencies between 10-' and 10-9.The gonococcal P-lactamase plasmids were first described in 1977 (19). Since that time, they have become endemic in North America, and they are common in other parts of the world (4, 8). They can be mobilized by the 24.5-megadalton (MDa) indigenous conjugative gonococcal plasmid, conjugative Haemophilus plasmids, and enteric plasmids (9,10,14,15,19). The 3.2-MDa 3-lactamase plasmid has also been associated with strains of Haemophilus ducreyi and urogenital Haemophilus parainfluenzae (1, 13). In contrast, the ,-lactamase plasmid was maintained in only a few strains of Neisseriaflava, Neisseria cinerea, Neisseria subflava, Neisseria mucosa, and N. subflava-N. sicca but not in strains of Neisseria meningitidis under laboratory conditions (8). Two reports (5, 16) indicated that ,-lactamase-producing N. meningitidis can be isolated, but this is a rare event. More recently, Ikeda et al. (10) found that 3 of 20 Neisseria gonorrhoeae strains (15%) carrying the 24.5-and 4.4-MDa plasmids mobilized the ,B-lactamase plasmid to N. meningitidis strains. In contrast, the 24.5-MDa gonococcal conjugative plasmid that mobilizes the P-lactamase plasmids has a narrow host range.The recent identification of the 25.2-MDa tetracycline plasmid in clinical isolates of N. gonorrhoeae, N. meningitidis, Kingella denitrificans, and Eikenella corrodens led to renewed concerns about the spread of the P-lactamase plasmids to N. meningitidis (11,15,20). Unlike the ancestral 24.5-MDa plasmid (8) occurring strains exist that carry the 25.2-and 4.4-MDa plasmids. Therefore, we created one by transferring a 25.2-MDa plasmid into a Proto/IB-3 recipient which carried the 4.4-MDa 3-lactamase and 2.6-MDa cryptic plasmids (CDC6). Both donors were naturally streptomycin resistant.The donor and recipient strains were grown in supplemented GCK broth for 4 h to a concentration of 108 CFU/mI, as previously described (19). Matings were performed by the filter-mating technique (19). Transconjugants were selected on GCK agar (Difco Laboratories, Detroit, Mich.) supplemented with rifampin (1 ,ug/ml) alone; rifampin (1 j,g/ml) and penicillin (10 ,ug/ml); rifampin (1 ,ug/ml) and tetracycline (10 jig/ml); or rifampin (1 jig/ml), penicillin (10 ,ug/ml), and tetracycline (10 ,ig/ml). Plates were incubated at 36.5°C in 4% CO2 and examined for the growth of the transconjugants at 24 and 48 h. The colonies that grew on the antibioticcontaining media were scored for nalidixic acid resistance. Colonies that were resistant to nalidixic acid and rifampin were considered to be true transconjugants.To verify the plasmid content of the transconjugants, selected isolates were screened by agarose gel electrophoresis, and plasmid profiles ...

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Isolation and molecular characterization of beta-lactamase-producing Haemophilus parainfluenzae from the genital tract

Cited by 9 publications

References 17 publications

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Mechanisms of antibiotic resistance inHaemophilus parainfluenzae

Transfer of beta-lactamase plasmids from Neisseria gonorrhoeae to Neisseria meningitidis and commensal Neisseria species by the 25.2-megadalton conjugative plasmid

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