Isolation and Molecular Characterization ofEscherichia coliO157 from Broiler and Human Samples

Abstract

There is a lack of information about the role of poultry, specifically chicken, in transmission of Escherichia coli (E. coli) O157 and subsequent human illnesses. This study was therefore aimed at investigating the presence of E. coli O157 and its virulence genes in various samples collected from broiler chickens and humans in Eastern Turkey by culture, immunomagnetic separation (IMS), and polymerase chain reaction (PCR). The genetic relationship between broiler and human isolates was also examined by pulsed-field gel electrophoresis (PFGE). In the PCR analysis of sorbitol-negative isolates, E. coli O157 was identified in 0.1% (1/1000) and 0.4% (4/1000) of the liver and cecum samples of broiler chickens, respectively. On the other hand, none of the carcass samples were determined to be positive for E. coli O157. Overall, the results indicated that 12% (3/25) of the flocks were positive for E. coli O157. The differences between the flocks in terms of the positivity were determined to be statistically significant (p<0.001). Ten (2.7%) of 367 human stool samples were also positive for E. coli O157 in the PCR examination. None of the broiler and human E. coli O157 isolates possessed H7, shigatoxins 1-2, or enterohemolysin genes, whereas all the broiler isolates and one of the human isolates were positive for intimin gene. In the PFGE analysis, a total of eight different profiles (four from broiler and four from human isolates) were observed. However, there were no genetic relationships between broiler and human E. coli O157 isolates. It can be concluded that more detailed studies are needed in poultry to better understand the role of these species in the epidemiology of E. coli 0157 infections in humans.

Isolation and molecular detection of respiratory bacterial agents from buffalo reared in some selected areas of Bangladesh

“…The isolates were also found as negative to MR test, VP test and positive to indole, catalase and oxidase test, as reported by Buxton and Fraser, (1977). Also in this study, colony characteristics of the isolated E. coli observed on NA, EMB and MacConkey agar were similar to the findings of Kalin et al, (2012); Nazir et al, (2005). The E. coli isolates revealed a complete fermentation of 5 basic sugars by producing both acid and gas which was supported by Thomas et al, (1998).…”

Isolation of <i>Escherichia coli</i> O157:H7 in Poultry by Culture, Serology and Polymerase Chain Reaction Technique in Jos, North Central Nigeria

“…In contrast to our findings, some positive results of isolation of E. coli O157:H7 have been reported by other researchers in poultry and other avian species. Kalin et al, (2012) had a 0.1% and 0.4% isolation of E. coli O157:H7 from liver and caecum samples respectively of 1000 chickens investigated in Turkey. All the isolates were positive for eaeA gene; a virulence factor responsible for human infections (Wang et al, 2002).…”

“…Ruminants especially cattle have been established as major reservoir of E. coli O157:H7 (Witold and Carolyn, 2011). In addition, some earlier studies (Dipineto et al, 2006;Kalin et al, 2012) reported isolation of E. coli O157:H7 in living layer hens and post mortem samples of broiler liver and caecum and from contaminated poultry meat and faeces (Olatoye et al, 2012;Aibinu et al, 2007). Some of the isolates from the aforementioned studies possessed hlyA (enterohaemolysin) and eaeA (intimin) genes which are known virulence factors of human pathogenic strains; bringing to the fore public health concerns of cross transmission (Wang et al, 2002;Rwego et al, 2008).…”

Tüketime Sunulan Kıymalarda Escherichia coli O157:H7’nin ve Bazı Virülens Genlerinin Araştırılması

“…Raw or undercooked meats may pose the agent. Although E. coli O157 has been found in animals such as cattle, sheep, pigs, and goats, studies indicated that poultry may also carry it and pose health risk to humans [11] .…”