Isolation and molecular characterization of some copper biosorped strains

Zaki, Sahar; Farag, Soha

doi:10.1007/bf03326164

Cited by 17 publications

(5 citation statements)

References 42 publications

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“…Conventional techniques for removing dissolved heavy metals in terms of chemical precipitation, carbon adsorption, electrolytic recovery, ion exchange, chelation and solvent extraction or liquid membrane separation had several disadvantages such as a high cost, incomplete removals, low selectivity, high energy consumption and generation of uneliminated toxic slurries. Of these reasons, the potential ability of microorganisms in the environmental protection and the toxic heavy metal recovery is of new promising approach in the removal of metal ions (Zaki and Farag 2010).…”

Section: Introductionmentioning

confidence: 99%

The potential capability of bacteria and yeast strains isolated from Rungkut Industrial Sewage in Indonesia as a bioaccumulators and biosorbents of copper

et al. 2017

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Abstract. Irawati W, Parhusip Ajn, Christian S, Yuwono T. 2017. The potential capability of bacteria and yeast strains isolated from Rungkut Industrial Sewage in Indonesia as a bioaccumulators and biosorbents of copper. . Heavy metal pollution is a serious problem as a result of industrialization due to the high production of wastewaters containing high concentrations of heavy metals. Wastewater contains microbial populations adapted to the toxic concentrations of heavy metals and becomes resistant by accumulating copper inside the cells. The aims of the study were to isolate yeast and bacteria from Rungkut Industrial sewage in Indonesia, and to examine the capability of these isolates to accumulate and biosorb copper. The copper resistance was determined by measuring minimum inhibitory concentration (MIC). The capability of isolates to accumulate and biosorb copper were determined by atomic absorption spectrophotometer. Biosorption is described as how much copper is removed from a growth medium with reference to the initial concentration. Of this study, nine bacterial strains and eight yeast strains were obtained with the MICs of 6-7 mM, and 16-20 mM CuSO 4 , respectively. Afterward, we have successfully selected three bacteria and one yeast strains showing the highest copper resistance. The three bacterial strains, designated as C1, C2, and C4 were able to accumulate copper up to 29.93, 508.01, 371.42 mg/cell dry weight, respectively. While the yeast strain, ES9.3 was able to accumulate 0.52 mg/cell dry weight and reduce up to 82.32% copper concentration in the medium. The findings of this study indicated that yeast and bacterial strains were promising microorganisms for removal of copper.

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Section: Introductionmentioning

confidence: 99%

The potential capability of bacteria and yeast strains isolated from Rungkut Industrial Sewage in Indonesia as a bioaccumulators and biosorbents of copper

et al. 2017

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“…Saat ini pengolahan limbah lebih diarahkan pada potensi bakteri dalam menyisihkan logam berat dari lingkungan atau disebut dengan bioremediasi. Bioremediasi logam berat menggunakan bakteri banyak mendapat perhatian karena memiliki nilai kebaharuan dan berpotensi untuk diterapkan dalam industri (Zaki & Farag, 2010). Bioremediasi merupakan teknologi pengolahan limbah dengan memanfaatkan sistem biologi untuk mengkatalisis biodegradasi atau transformasi berbagai senyawa toksik menjadi bentuk yang kurang toksik.…”

Section: Pendahuluanunclassified

Multiresistensi dan Akumulasi Acinetobacter sp. IrC2 terhadap Logam Berat

Irawati

Hasthosaputro

Kusumawati³

2020

J. Biol Papua

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The increasing industrial activity in Indonesia, that is not equipped with appropriate waste treatment, has caused an increase of heavy metal contaminants in water bodies. Heavy metals contamination such as copper (Cu), mercury (Hg), cadmium (Cd), and lead (Pb) contamination in water bodies have endangered aquatic life and public health. For this reason, it is urgently important to lower down the concentration of heavy metal pollutants in the water bodies surrounding industrial areas. Compared to chemical remediation, bioremediation of heavy metal by using indigenous bacteria is more effective and economical, since it can be applied in situ directly and be used repeatedly. Acinetobacter sp. IrC2, used in this study, is Indonesian indigenous bacteria isolated from the industrial waste treatment facility in Rungkut, Surabaya. This study aims, firstly, to investigate the heavy metal multiresistance of Acinetobacter sp. IrC2 against mercury, cadmium, and lead. Secondly, this study intends to examine its bioaccumulation capacity for single and heavy metal alloys. The heavy metal multiresistance test was carried out by measuring the minimum heavy metal concentrations that inhibit bacterial growth (Minimum Inhibitory Concentration/MIC). The bioaccumulation capacity was measured using an atomic absorption spectrophotometer (AAS). It is shown that Acinetobacter sp. IrC2 has high multiresistance to mercury, cadmium, and lead with MIC values of 12 mM, 8 mM, and 18 mM, respectively. Furthermore, it is also resistant to heavy metal mixture of 4.5 mM. The mechanism of bacterial resistance in response to heavy metal toxicity, in general, is by accumulating heavy metals in the cells. The highest amount of accumulated heavy metals identified, from bacteria grown in the medium contains a mixture of heavy metals, were 0.023 mg, 0.084 mg, 0.684 mg, and 1.476 mg per gram of cell dry weight for copper, mercury, cadmium and lead respectively. In conclusion, Acinetobacter sp. IrC2 is a promising heavy metal bioremediation agent due to its heavy metal multiresistance and accumulator characteristics. Key words: Acinetobacter sp. IrC2; cadmium; copper; lead; merkuri

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“…This is probably due to larger surface area of the E-MKD particle being available at beginning for the adsorption of copper ions. As the surface adsorption sites become exhausted, the uptake rate is controlled by the rate at which the adsorbate is transported from the exterior to the interior sites of the adsorbent particles (Zaki and Farag 2010). Adsorption first followed a linear rising, and then stationary state was observed following the rapid decrease in copper ions removal.…”

Section: Effect Of Contact Timementioning

confidence: 99%

Equilibrium studies of copper ion adsorption onto modified kernel of date (Fructus dactylus)

Nadaroğlu

Kalkan

Çelik

2014

Int. J. Environ. Sci. Technol.

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Heavy metal pollution of water is a serious problem because of its toxic effects on the human health and living organism. Therefore, there is a need to develop technologies to remove toxic pollutants found in polluted water. There are different ways to remove the toxic metals from wastewater. Among all treatments proposed, the adsorption process is one of the most promising technologies in water pollution control. Recently, numerous approaches have been studied for the development of cheaper and effective adsorbents. Many low-cost adsorbents, including natural materials, biosorbents and waste materials from industry and agriculture, have been proposed by several researchers. In this study, removal of copper from aqueous solution by using powdered and modified kernel of date (Fructus dactylus) with apocarbonic anhydrase enzyme has been investigated. The operating variables studied were contact time, initial solution pH, initial metal concentration, temperature and adsorbent dosage. The experimental investigation results showed that modified and powdered kernel of date (F. dactylus) has a high level of adsorption capacity for copper ions. The adsorption data were correlated with the Langmuir and Freundlich isotherm models. It was found that the Langmuir and Freundlich isotherms fitted well to the data. The results revealed that copper is considerably adsorbed on modified and powdered kernel of date (F. dactylus). Consequently, it is concluded that the modified and powdered kernel of date (F. dactylus) can be successfully used for the removal of the copper ions from the aqueous solutions.

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Isolation and molecular characterization of some copper biosorped strains

Cited by 17 publications

References 42 publications

The potential capability of bacteria and yeast strains isolated from Rungkut Industrial Sewage in Indonesia as a bioaccumulators and biosorbents of copper

The potential capability of bacteria and yeast strains isolated from Rungkut Industrial Sewage in Indonesia as a bioaccumulators and biosorbents of copper

Multiresistensi dan Akumulasi Acinetobacter sp. IrC2 terhadap Logam Berat

Equilibrium studies of copper ion adsorption onto modified kernel of date (Fructus dactylus)

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