Isolation and Molecular Docking Studies of Dihydroimperialine as Butyrylcholinesterase Inhibitor from the Bulbs of Fritillaria Imperialis

Abstract

Alzheimer's disease is a progressive disorder that causes the inhibition of neurotransmitter acetylcholine in brain cells. Fritillaria imperials belong to family liliaceae, comprises several steroidal alkaloids. The subsequent extraction of ethanoic extract was subjected to column and thin layer chromatography, which led to the isolation of two steroidal alkaloids, Dihydroimperialine (1) and imperialine (2). Dihydroimperialine (1) exhibited the acetylcholinesterase and butyrylcholinestearse inhibitory activities with IC50 values as (>500 ± 0.23) and (14.40 ± 1.02), respectively. Compound 1 and imperialine (2) inhibited cholinesterase enzymes in a concentration-dependent manner. Additionally, molecular docking studies were also performed to investigate the binding mode of dihydroimperialine (1) with butyrylcholinestearse. The ligand-BChE complex were found to be stabilized by hydrophobic contacts, hydrogen bonding, and π-π stacking between the compounds and amino acid residues. The main object of this study to investigate the acetylcholinesterase and butyrlcholinestrase inhibitory activity from the bulb of F. imperials. This is the first report of the isolation of dihydroimperialine (1) form natural source.

Phytochemical Analysis and GC-MS Profiling in the Flower of<i> Plumeria alba</i>

“…Thus, the phytochemical compounds of P. alba flower were assessed and identified in this study. More recently, we published the biological activity of Dihydroimperialine from the bulbs of Fritillaria Imperialis for Alzheimer's disease and cytotoxicity property of Boesenbergia rotunda medicinal plants [9,10].…”