Isolation and molecular identification of rare coagulase-negative <i>Staphylococcus Aureus</i> Variants Isolated from Bovine milk samples

Sundareshan, S.; Isloor, S.; Babu, Y. Hari; Sunagar, Raju; Sheela, P.; Tiwari, J.G.; Wyryah, C.B.; Mukkur, T.K.S.; Hegde, Nagendra R.

doi:10.5958/0974-0147.2017.00011.3

Cited by 6 publications

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“…Although not without limitations [ 3 ], these tests are widely employed across global communities of milk producers. We have also reported on the use of such tests in the detection of bovine SCM [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

et al. 2015

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Buffaloes are the second largest source of milk. Mastitis is a major impediment for milk production, but not much information is available about bubaline mastitis, especially subclinical mastitis. The aim of this study was to (a) investigate the application of various tests for the diagnosis of bubaline subclinical mastitis, (b) identify the major bacteria associated with it, and (c) evaluate the antibiotic resistance pattern of the bacteria. To this end, 190 quarter milk samples were collected from 57 domesticated dairy buffaloes from organized (64 samples) and unorganized (126 samples) sectors. Of these, 48.4%, 40.0%, 45.8%, 61.1%, and 61.6% were positive for subclinical mastitis by somatic cell count, electrical conductivity, California mastitis test, bromothymol blue test, and N-acetyl glucosaminidase test, respectively. As compared to the gold standard of somatic cell count, California mastitis test performed the best. However, a combination of the two methods was found to be the best option. Microbiological evaluation, both by biochemical methods as well as by monoplex and multiplex polymerase chain reaction, revealed that coagulase-negative staphylococci were the most predominant (64.8%) bacteria, followed by streptococci (18.1%), Escherichia coli (9.8%) and Staphylococcus aureus (7.3%). Most of the pathogens were resistant to multiple antibiotics, especially to β-lactam antibiotics. We propose that California mastitis test be combined with somatic cell count for diagnosis of subclinical mastitis in domestic dairy buffaloes. Further, our results reveal high resistance of the associated bacteria to the β-lactam class of antibiotics, and a possible major role of coagulase-negative staphylococci in causing the disease in India.

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Section: Introductionmentioning

confidence: 99%

Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

et al. 2015

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“…These coagulase-variable strains are more frequently found in some species than others, but their misdiagnosis may lead to unsuitable treatment of infections and control measures. This is especially significant when the detection of the pathogenic S. aureus relies on coagulase production and strains of these species do not produce coagulase, leading to isolate misidentification (Sundareshan et al, 2017). Strains of the CoNS S. chromogenes, S. xylosus, S. cohnii and S. agnetis have been reported to clot plasma, leading to misidentification of the pathogens causing mastitis in dairy animals (Taponen et al, 2012;Santos et al, 2016;Almeida et al, 2018).…”

Section: Antimicrobial Resistance Increase and The Interconnectednessmentioning

confidence: 99%

Underrated Staphylococcus species and their role in antimicrobial resistance spreading

2020

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The increasing threat of antimicrobial resistance has shed light on the interconnection between humans, animals, the environment, and their roles in the exchange and spreading of resistance genes. In this review, we present evidences that show that Staphylococcus species, usually referred to as harmless or opportunistic pathogens, represent a threat to human and animal health for acting as reservoirs of antimicrobial resistance genes. The capacity of genetic exchange between isolates of different sources and species of the Staphylococcus genus is discussed with emphasis on mobile genetic elements, the contribution of biofilm formation, and evidences obtained either experimentally or through genome analyses. We also discuss the involvement of CRISPR-Cas systems in the limitation of horizontal gene transfer and its suitability as a molecular clock to describe the history of genetic exchange between staphylococci.Given the increasing recognition of the importance of previously overlooked Staphylococcus species, the goal of this review was to present evidences that put these bacteria in the front row of resistance dissemination and highlight their potential threat to human and animal health.

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“…In addition, coagulase-negative variants of S. aureus are known to exist, some of which can have similar pathogenicity to their coagulase-positive variants (3). Some S. aureus isolates of bovine origin react negatively to the standard coagulase test and are PCR-negative for the coa gene (4,5). Additionally, the von Willebrand factor-binding protein exhibits coagulating ability, resulting in S. aureus producing two proteins that coagulate plasma (6).…”

Section: Introductionmentioning

confidence: 99%

Non-aureus Staphylococci and Bovine Udder Health: Current Understanding and Knowledge Gaps

Buck

Naushad

et al. 2021

Front. Vet. Sci.

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Despite considerable efforts to control bovine mastitis and explain its causes, it remains the most costly and common disease of dairy cattle worldwide. The role and impact of non-aureus staphylococci (NAS) in udder health are not entirely understood. These Gram-positive bacteria have become the most frequently isolated group of bacteria in milk samples of dairy cows and are associated with (mild) clinical and subclinical mastitis. Different species and strains of NAS differ in their epidemiology, pathogenicity, virulence, ecology and host adaptation, and antimicrobial resistance profiles. They have distinct relationships with the microbiome composition of the udder and may also have protective effects against other mastitis pathogens. Some appear to persist on the skin and in the teat canal and udder, while others seem to be transient residents of the udder from the environment. Analyzing genotypic and phenotypic differences in individual species may also hold clues to why some appear more successful than others in colonizing the udder. Understanding species-level interactions within the microbiome and its interactions with host genetics will clarify the role of NAS in bovine mastitis and udder health.

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Isolation and molecular identification of rare coagulase-negative Staphylococcus Aureus Variants Isolated from Bovine milk samples

Cited by 6 publications

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Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

Isolation, Biochemical and Molecular Identification, and In-Vitro Antimicrobial Resistance Patterns of Bacteria Isolated from Bubaline Subclinical Mastitis in South India

Underrated Staphylococcus species and their role in antimicrobial resistance spreading

Non-aureus Staphylococci and Bovine Udder Health: Current Understanding and Knowledge Gaps

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