Isolation and Molecular Identification of Clostridium perfringens Type D in Goats in District Peshawar

Shehzadi, Sidra; Khan, Sher Bahadar; Sadique, Umer; Nawaz, Saqib

doi:10.17582/journal.sja/2021/37.1.110.114

Cited by 1 publication

(3 citation statements)

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“…As the present study was conducted in the winter month, similarly, Shehzadi et al (20) also conducted a study in the spring months (February to April). Two unusual factors in the present study were noted, i.e., (i) animals grazing on oats and (ii) heavy rain in cold weather.…”

Section: Discussionmentioning

confidence: 68%

“…This study reported an outbreak of enterotoxemia in goats kept under tropical conditions. It is a common and economically important disease worldwide (9)(10)(11)20). Peracute and acute mortality have earlier been reported (19,42,43).…”

Section: Discussionmentioning

confidence: 99%

“…Enterotoxemia caused by Clostridium perfringens has a worldwide distribution. It has been reported from Brazil (12,13), China (14)(15)(16), Italy (17,18), Pakistan (19,20), Spain (21), Saudi Arabia (22), South Africa (23), and USA (24,25).…”

Section: Introductionmentioning

confidence: 99%

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Clostridium perfringens Types A and D Involved in Peracute Deaths in Goats Kept in Cholistan Ecosystem During Winter Season

et al. 2022

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Enterotoxemia is a severe and peracute disease caused by Clostridium perfringens (C. perfringens) rendering high mortality leading to huge economic losses, especially in small ruminants. The bacterium induces peracute death in animals based on the rapid production of different lethal toxins. Mortality occurred three private herds of two breeds, i.e., Makhi Cheeni and Beetal, and one non-descriptive (Teddy) herds reared in the desert area of Bahawalpur, Pakistan. At necropsy, tissue samples for histopathology and intestinal contents for bacterial isolation and culture were collected. Following the standard procedure, tissue slides were prepared. Multiplex PCR was used to identify toxinotypes using specific primers. Morbidity, mortality, and case fatality in Makhi Cheeni, Beetal, and Teddy goats caused by enterotoxemia were 87.58, 75.81, and 76.11%, respectively. Based on toxinotypes in the present outbreaks, C. perfringens type A (cpα = 20.7%; cpα + cpβ2 = 11.2%) and C. perfringens type D (cpα + cpβ2 + etx = 47.7%; cpα + etx = 20.7%) were detected. Deaths due to C. perfringens type D (68.10%) were significantly higher (p < 0.001) compared with deaths by C. perfringens type A (34.90%). Petechiation of serosal surfaces, hemorrhage of intestines, lungs, and liver were seen. Kidneys were soft, and under the microscope, tubules were studded with erythrocytes. There was stunting and fusion in the intestinal villi. From this study, we concluded that endotoxemia can occur in any season; thus, a proper vaccination schedule must be followed for the protection of small ruminants' health.

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Section: Discussionmentioning

confidence: 68%